An initial limited biodosimetry inter-comparison exercise: FOI and DRDC Ottawa

Stricklin, Daniela; Wilkinson, Diana; Arvidsson, E.; Prud'homme-Lalonde, Louise; Thorleifson, Erika; Mullins, Dana; Lachapelle, Sylvie

doi:10.1016/j.radmeas.2007.05.043

Cited by 6 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Authors have stated that changes in the laboratory culture protocol and handling of samples can affect metaphase chromosomes. 24,25 Because of this factor, each cytogenetic laboratory is advised to construct their dose-response curves and coefficients. 22 As of this time, the data of this report is still preliminary.…”

Section: Discussionmentioning

confidence: 99%

Interim In vitro Dose-Response Curve for the Dicentric Biodosimeter Assay from a Philippine Radiotherapy Facility using a Linear Accelerator

Guzman¹,

Padilla²,

Co³

et al. 2021

Acta Med Philipp

View full text Add to dashboard Cite

Background. Accidental radiation exposure can occur anytime. Biodosimeters help in quantifying the absorbed dose of individuals who are not equipped with personal dosimeters during radiation exposure. The dicentric assay can quantify radiation damage by correlating radiation dose exposure with the frequency of dicentric chromosomes in the peripheral lymphocytes extracted from exposed individuals. Objective. The study aims to present the interim results of the reference dose-response curve for a Philippine radiotherapy facility constructed using a 6MV linear accelerator (ClinacX, Varian). Methods. Samples of peripheral blood from healthy volunteers were irradiated in a customized water phantom of doses 0.10 to 5.0 Gray using a linear accelerator. The irradiated samples were cultured and analyzed following the International Atomic Energy Agency Cytogenetic Dosimetry Protocol (2011) with modifications. Linear-quadratic model curve fitting and further statistical analysis were done using CABAS (Chromosome Aberration Calculation Software Version 2.0) and Dose Estimate (Version 5.2). Interim results of the samples were used to generate these curves. Results. The dose-response curve generated from the preliminary results were comparable to published dose response curves from international cytogenetic laboratories. Conclusion. The generated dose-response calibration curve will be useful for medical triage of the public and radiologic staff accidentally exposed to radiation during medical procedures or in the event of nuclear accidents.

show abstract

Section: Discussionmentioning

confidence: 99%

Interim In vitro Dose-Response Curve for the Dicentric Biodosimeter Assay from a Philippine Radiotherapy Facility using a Linear Accelerator

Guzman¹,

Padilla²,

Co³

et al. 2021

Acta Med Philipp

View full text Add to dashboard Cite

show abstract

“…In an alternative experiment, irradiation (0.7 Gy/min), culture and slide preparation were carried out in lab II and then sent to lab I. This was done to reduce the variation in sample processing, as it has been demonstrated that slight differences in processing and handling of samples in a lab can impact the quality of metaphase chromosomes (Stricklin et al 2007). Various types of CA were recorded by manual microscopic scoring by two independent scorers.…”

mentioning

confidence: 99%

“…It has been reported that the qualitative nature of selection of metaphases for DC scoring could result in differences in scoring between laboratories. Alternatively, within a lab, an evaluator becomes accustomed to his or her own quality of metaphase spreads resulting in reliable analysis (Stricklin et al 2007).…”

mentioning

confidence: 99%