An injectable anisotropic alginate hydrogel containing oriented fibers for nerve tissue engineering

Ghaderinejad, Paria; Najmoddin, Najmeh; Bagher, Zohreh; Saeed, Mahdi; Karimi, Sarah; Simorgh, Sara; Pezeshki‐Modaress, Mohamad

doi:10.1016/j.cej.2021.130465

Cited by 79 publications

(62 citation statements)

References 41 publications

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“…Similar research was conducted by Ghaderinejad et al [185]. The injectable alginate hydrogel system was loaded with magnetic short PCL/superparamagnetic iron oxide nanoparticle (SPION) electrospun fibers.…”

Section: Hydrogels and Electrospun Nanofibersmentioning

confidence: 68%

Hydrogel, Electrospun and Composite Materials for Bone/Cartilage and Neural Tissue Engineering

2021

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Injuries of the bone/cartilage and central nervous system are still a serious socio-economic problem. They are an effect of diversified, difficult-to-access tissue structures as well as complex regeneration mechanisms. Currently, commercially available materials partially solve this problem, but they do not fulfill all of the bone/cartilage and neural tissue engineering requirements such as mechanical properties, biochemical cues or adequate biodegradation. There are still many things to do to provide complete restoration of injured tissues. Recent reports in bone/cartilage and neural tissue engineering give high hopes in designing scaffolds for complete tissue regeneration. This review thoroughly discusses the advantages and disadvantages of currently available commercial scaffolds and sheds new light on the designing of novel polymeric scaffolds composed of hydrogels, electrospun nanofibers, or hydrogels loaded with nano-additives.

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Section: Hydrogels and Electrospun Nanofibersmentioning

confidence: 68%

Hydrogel, Electrospun and Composite Materials for Bone/Cartilage and Neural Tissue Engineering

2021

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“…6(F). 13 Another formulation of a polyvinyl alcohol/sulfated alginate nanofibrous scaffold with 30 wt% alginate concentration also exhibited a desirable surface attachment for Schwann cells and human bone marrow mesenchymal cells. 286 Studies showed that a gelatin-alginate formulation can be 3D printed by itself 287 or further mixed with different concentrations of carbon nanofibers and printed into an electroconductive hydrogel with desirable electrical, mechanical, and biocompatible characteristics.…”

Section: Agarosementioning

confidence: 99%

Carbohydrate based biomaterials for neural interface applications

Dhawan

Cui

2022

J. Mater. Chem. B

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“…Nasal mucosa cells were isolated according to the described method in previous research. 45,46 Briefly, after obtaining approval from the ethics committee and consent from the patient, the olfactory mucosae was taken from patients under a local anesthetic at Iran University Hospital. Isolated specimens were washed with PBS supplemented antibiotic 1% penicillin-streptomycin (Pen/Strep; Gibco) several times and placed in Dulbecco's modified Eagle's medium/ F12 (DMEM/F12; Gibco).…”

Section: Isolation and Characterization Of Human Olfactory Ecto-mesen...mentioning

confidence: 99%

Three‐dimensional‐printed polycaprolactone/polypyrrole conducting scaffolds for differentiation of human olfactory ecto‐mesenchymal stem cells into Schwann cell‐like phenotypes and promotion of neurite outgrowth

Entezari

Mozafari

Bakhtiyari

et al. 2022

J Biomedical Materials Res

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Implantation of a suitable nerve guide conduit (NGC) seeded with sufficient Schwann cells (SCs) is required to improve peripheral nerve regeneration efficiently. Given the limitations of isolating and culturing SCs, using various sources of stem cells, including mesenchymal stem cells (MSCs) obtained from nasal olfactory mucosa, can be desirable. Olfactory ecto-MSCs (OE-MSCs) are a new population of neural crestderived stem cells that can proliferate and differentiate into SCs and can be considered a promising autologous alternative to produce SCs. Regardless, a biomimetic physicochemical microenvironment in NGC such as electroconductive substrate can affect the fate of transplanted stem cells, including differentiation toward SCs and nerve regeneration. Therefore, in this study, the effect of 3D printed polycaprolactone (PCL)/polypyrrole (PPy) conductive scaffolds on differentiation of human OE-MSCS into functional SC-like phenotypes was investigated. Biological evaluation of 3D printed scaffolds was examined by in vitro culturing the OE-MSCs on samples surfaces, and conductivity showed no effect on increased cell attachment, proliferation rate, viability, and distribution. In contrast, immunocytochemical staining and real-time polymerase chain reaction analysis indicated that 3D structures coated with PPy could provide a favorable microenvironment for OE-MSCs differentiation. In addition, it was found that differentiated OE-MSCs within PCL/PPy could secrete the highest amounts of nerve growth factor and brain-derived neurotrophic factor neurotrophic factors compared to pure PCL and 2D culture. After co-culturing with PC12 cells, a significant increase in neurite outgrowth on PCL/PPy conductive scaffold seeded with differentiated OE-MSCs. These findings indicated that 3D printed PCL/PPy conductive scaffold could support differentiation of OE-MSCs into SC-like phenotypes to promote neurite outgrowth, suggesting their potential for neural tissue engineering applications.

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An injectable anisotropic alginate hydrogel containing oriented fibers for nerve tissue engineering

Cited by 79 publications

References 41 publications

Hydrogel, Electrospun and Composite Materials for Bone/Cartilage and Neural Tissue Engineering

Hydrogel, Electrospun and Composite Materials for Bone/Cartilage and Neural Tissue Engineering

Carbohydrate based biomaterials for neural interface applications

Three‐dimensional‐printed polycaprolactone/polypyrrole conducting scaffolds for differentiation of human olfactory ecto‐mesenchymal stem cells into Schwann cell‐like phenotypes and promotion of neurite outgrowth

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