An injectable conductive hydrogel encapsulating plasmid DNA-eNOs and ADSCs for treating myocardial infarction

Wang, Wei; Tan, Baoyu; Chen, Jingrui; Bao, Rui; Zhang, Xuran; Liang, Shuang; Shang, Yingying; Liang, Wei; Cui, Yuan‐Lu; Fan, Guanwei; Jia, Hui‐Zhen; Liu, Wenguang

doi:10.1016/j.biomaterials.2018.01.021

Cited by 160 publications

(145 citation statements)

References 79 publications

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“…The field has come very far in recent years, due to the cooperation of biologists, chemists and engineers. One standout therapy by Wang et al took a holistic approach to heal myocardial infarction, aiming to combine an injectable hydrogel with a tetraaniline conductive element, stem cells and genes. Incremental addition of each component shows steady, synergistic improvements in regenerated heart tissue.…”

Section: Discussionmentioning

confidence: 99%

Conductive Scaffolds for Cardiac and Neuronal Tissue Engineering: Governing Factors and Mechanisms

Burnstine‐Townley

Eshel

Amdursky

2019

Adv Funct Materials

118

102

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Tissue engineering is a promising therapeutic approach in medicine, targeting the replacement of a diseased tissue with a healthy one grown within an artificial scaffold. Due to the high prevalence of cardiac and brain‐related ailments that involve some necrosis of tissue, cardiac and neuronal tissue engineering are intensely studied fields in regenerative medicine. A growing trend in the use of conductive scaffolds for the growth of these tissues has been witnessed recently. While the results are irrefutable, the mechanism of how an electrically conducting scaffold interacts with an electroactive tissue remains has remained elusive. An up‐to‐date summary of all work done in the field is reported, with a special focus on the specific contribution of the conductive scaffold on the performance of the formed tissue. The cell–scaffold electronic interface is then explored from an electrical engineering perspective. The electronic configuration of the system and the mechanisms and governing factors controlling the ability of a conductive scaffold to support cardiac and neuronal tissues are discussed. Using several simulations, the required conductivity of the scaffold in order for it to be suitable for tissue engineering—which also depends on the nature of the charge carriers—is also discussed.

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Section: Discussionmentioning

confidence: 99%

Conductive Scaffolds for Cardiac and Neuronal Tissue Engineering: Governing Factors and Mechanisms

Burnstine‐Townley

Eshel

Amdursky

2019

Adv Funct Materials

118

102

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“…These fibers have the potential to deliver and receive electrical signals to cells, thereby showing great promise in a wide number of fields, as these mechanisms are known to affect cell behaviors such as differentiation, orientation, mobility, and more (Sirivisoot et al, 2014;Wang et al, 2017;Osipova et al, 2018;Zhang et al, 2018). Current conductive hydrogels in biomedical fields take the form of membranes (Escalona et al, 2012), gels (Liu et al, 2016;Bu et al, 2018), or films (Dong et al, 2016;Liu et al, 2017;Wang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Introduction of graphene into alginate microfibers significantly increased the conductivity of the resulting hydrogels by a factor of 2.5, achieving an overall conductivity of 0.0025 S m −1 for dry fibers mounted using carbon tape and colloidal silver paste (Figure 4). This indicates that the graphene flakes within the fibers are consistently dispersed enough to shorten diffusion distance of electrons, thereby aiding in the transportation of electrons through the bulk of the fiber and increasing its electrical conductivity (Wu and Zhong, 2019) Other cell-laden conductive hydrogels, which have thus far taken the form of films, achieved conductivities of 0.2 S m −1 (Dong et al, 2016) and 0.02 S m −1 (Wang et al, 2018) for wet samples. These films are based on synthetic polymers and were biocompatible, but lacked the high degree of control over cell culture geometry that microfibers can provide.…”

Section: Conductivitymentioning

confidence: 99%

Enhancing the Conductivity of Cell-Laden Alginate Microfibers With Aqueous Graphene for Neural Applications

et al. 2020

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Microfluidically manufacturing graphene-alginate microfibers create possibilities for encapsulating rat neural cells within conductive 3D tissue scaffolding to enable the creation of real-time 3D sensing arrays with high physiological relavancy. Cells are encapsulated using the biopolymer alginate, which is combined with graphene to create a cell-containing hydrogel with increased electrical conductivity. Resulting novel alginate-graphene microfibers showed a 2.5-fold increase over pure alginate microfibers, but did not show significant differences in size and porosity. Cells encapsulated within the microfibers survive for up to 8 days, and maintain ∼20% live cells over that duration. The biocompatible aqueous graphene suspension used in this investigation was obtained via liquid phase exfoliation of pristine graphite, to create a graphene-alginate pre-hydrogel solution.

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“…Recent years, heart failures after myocardial infarction is one of the primary causes of human mortality worldwide, and creating a therapeutic challenge for biomedical researchers [1,2]. The native myocardial tissue is a main part of transferring electrical signal and in uencing factor of heart beat functions, while myocardial infarction leads to unwanted scar formations of myocardial tissues [3][4][5]. The damage of myocardium is chief causes of mechanical and electrical signal loss, which leads to weakened cardiac functions and consequently made heart failures.…”

Section: Introductionmentioning

confidence: 99%

Development and novel design of clustery Graphene Oxide formed Conductive Silk hydrogel cell vesicle to Repair Myocardial Infarction: Investigation of its biological activity for cell delivery applications

Duan²,

Guo

2020

Preprint

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Cell delivery therapy is a talented and hopeful strategic approach to repair the damaged cardiac tissues after myocardial infarction. The biocompatible injectable hydrogel with bioactive nanomaterials with effective self-healing capability has been highly required for the cell delivery and regeneration therapy. In the present study, we have developed the biopolymeric and biocompatible self-healing silk protein hydrogel matrix with graphene oxide nanoformulations as cell delivery vehicles for the treatment of myocardial infarction regeneration. The materials combinations, structural interactions, thermal stability and morphology of the designed hydrogel were investigated and elaborated. To improve therapeutic potential of the hydrogel matrix, growth factor was loaded and investigated its biological activity such as cell survival and cell proliferations properties by using endothelial progenitor cells. Collected, these developed materials are excellent vesicle for cell therapy in myocardial infarction.

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An injectable conductive hydrogel encapsulating plasmid DNA-eNOs and ADSCs for treating myocardial infarction

Cited by 160 publications

References 79 publications

Conductive Scaffolds for Cardiac and Neuronal Tissue Engineering: Governing Factors and Mechanisms

Conductive Scaffolds for Cardiac and Neuronal Tissue Engineering: Governing Factors and Mechanisms

Enhancing the Conductivity of Cell-Laden Alginate Microfibers With Aqueous Graphene for Neural Applications

Development and novel design of clustery Graphene Oxide formed Conductive Silk hydrogel cell vesicle to Repair Myocardial Infarction: Investigation of its biological activity for cell delivery applications

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