An injectable extracellular matrix derived hydrogel for meniscus repair and regeneration

Wu, Jinglei; Ding, Qing; Dutta, Ahana; Wang, Yezhou; Huang, Yuheng; Hong, Wenting; Tang, Liping; Hong, Yi

doi:10.1016/j.actbio.2015.01.027

Cited by 171 publications

(198 citation statements)

References 75 publications

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“…The compression testing of cylinder hydrogels (4 mm height; 9 mm diameter; n = 4) was performed on MTS Insight Testing System with a 500 N load cell and a cross-head rate of 1 mm/min. 29 For uniaxial tensile testing, the strips of PEG–PCL-DA hydrogels with 50 mm length, 5 mm width, and 3 mm height ( n = 3) were prepared and tested on MTS Insight Testing System with a 500 N load cell and a cross-head rate of 10 mm/min according to ASTM D638-03. 30 The instant strain recovery of PEG–PCL-DA hydrogel strips ( n = 4) were carried out and measured under the same conditions as described above.…”

Section: Methodsmentioning

confidence: 99%

Highly Elastic Biodegradable Single-Network Hydrogel for Cell Printing

Lee

Dai

et al. 2018

ACS Appl. Mater. Interfaces

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Cell printing is becoming a common technique to fabricate cellularized printed scaffold for biomedical application. There are still significant challenges in soft tissue bioprinting using hydrogels, which requires live cells inside the hydrogels. Moreover, the resilient mechanical properties from hydrogels are also required to mechanically mimic the native soft tissues. Herein, we developed a visible-light cross-linked, single-network, biodegradable hydrogel with high elasticity and flexibility for cell printing, which is different from previous highly elastic hydrogel with double-network and two components. The single-network hydrogel using only one stimulus (visible light) to trigger gelation can greatly simplify the cell printing process. The obtained hydrogels possessed high elasticity, and their mechanical properties can be tuned to match various native soft tissues. The hydrogels had good cell compatibility to support fibroblast growth in vitro. Various human cells were bioprinted with the hydrogels to form cell–gel constructs, in which the cells exhibited high viability after 7 days of culture. Complex patterns were printed by the hydrogels, suggesting the hydrogel feasibility for cell printing. We believe that this highly elastic, single-network hydrogel can be simply printed with different cell types, and it may provide a new material platform and a new way of thinking for hydrogel-based bioprinting research.

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Section: Methodsmentioning

confidence: 99%

Highly Elastic Biodegradable Single-Network Hydrogel for Cell Printing

Lee

Dai

et al. 2018

ACS Appl. Mater. Interfaces

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“…Turbidimetric gelation kinetics and rheology are the primary methods used to assess the viscoelastic properties of ECM hydrogels. Other methods, such as indentation [69] and compression [46, 64, 70] testing, AFM [65], and macroscopic rigidity [20, 23, 71] have been explored but will not be further reviewed herein.…”

Section: Ecm Hydrogel Characterizationmentioning

confidence: 99%

“…Sigmoidal gelation behavior is also observed with bone [72], cartilage [70] and spinal cord ECM [54] hydrogels, whereas brain ECM hydrogels [54] show exponential behavior. The lag phase (t lag ) and the time to reach half of the final turbidity (t 1/2 ) is greater in UBM than collagen I gels, ostensibly due to the presence of GAGs and other molecules that may modulate self-assembly [20].…”

Section: Ecm Hydrogel Characterizationmentioning

confidence: 99%

“…The lag phase (t lag ) and the time to reach half of the final turbidity (t 1/2 ) is greater in UBM than collagen I gels, ostensibly due to the presence of GAGs and other molecules that may modulate self-assembly [20]. The t lag and t 1/2 vary with gelation mechanism [43, 44] and concentration [23, 71] in some cases, and are concentration-independent in others [70]. …”

Section: Ecm Hydrogel Characterizationmentioning

confidence: 99%

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Extracellular matrix hydrogels from decellularized tissues: Structure and function

et al. 2017

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Extracellular matrix (ECM) bioscaffolds prepared from decellularized tissues have been used to facilitate constructive and functional tissue remodeling in a variety of clinical applications. The discovery that these ECM materials could be solubilized and subsequently manipulated to form hydrogels expanded their potential in vitro and in vivo utility; i.e. as culture substrates comparable to collagen or Matrigel, and as injectable materials that fill irregularly-shaped defects. The mechanisms by which ECM hydrogels direct cell behavior and influence remodeling outcomes are only partially understood, but likely include structural and biological signals retained from the native source tissue. The present review describes the utility, formation, and physical and biological characterization of ECM hydrogels. Two examples of clinical application are presented to demonstrate in vivo utility of ECM hydrogels in different organ systems. Finally, new research directions and clinical translation of ECM hydrogels are discussed.

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“…Creating an ECM based material that can be delivered by injection expands the benefits of ECM to a wider variety of applications including myocardial infarction [6], reconstruction of skeletal muscle [7], and musculoskeletal applications [8], urinary incontinence [9], adipose tissue engineering [10], and orthopaedic applications such as meniscus repair [11]. Here we developed an injectable ECM-based tissue construct for musculoskeletal tissue engineering applications such as post-traumatic osteoarthritis (PTOA), rheumatoid arthritis, and osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

Homogenized Porcine Extracellular Matrix Derived Injectable Tissue Construct with Gold Nanoparticles for Musculoskeletal Tissue Engineering Applications

Smith¹,

Snider²,

Gilley³

et al. 2017

JBNB

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A unique porcine extracellular matrix (ECM) derived injectable tissue construct with 100 nm or 20 nm gold nanoparticles (AuNP) was developed for musculoskeletal tissue engineering applications. ECM has been shown to encourage cellularity and tissue remodeling due to its release of growth factors while AuNP have been shown to reduce reactive oxygen species (ROS) levels. Injectable tissue constructs were created by homogenizing decellularized porcine diaphragm tendon conjugated with 100 nm or 20 nm AuNP at 1x, 4x, and 8x concentrations. Extrusion force testing demonstrated that homogenized tissue constructs were injectable at an appropriate cannula size and force. L-929 murine fibroblasts were used to measure cell viability, cell proliferation, intracellular ROS levels, and cell migration in response to constructs. Enhanced cell viability and proliferation are observed on 1 × 20 nm AuNP constructs. ROS assays demonstrate reduced cellular ROS concentrations from all 20 nm AuNP constructs and from 8 × 100 nm AuNP constructs compared with constructs without nanoparticles. Cellular migration is higher towards 4 × 20 nm AuNP constructs compared with constructs without nanoparticles. Results support the potential use of a porcine ECM derived injectable tissue construct with AuNP as an injectable tissue construct to reduce inflammation and to promote tissue remodeling in musculoskeletal tissue engineering applications.

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An injectable extracellular matrix derived hydrogel for meniscus repair and regeneration

Cited by 171 publications

References 75 publications

Highly Elastic Biodegradable Single-Network Hydrogel for Cell Printing

Highly Elastic Biodegradable Single-Network Hydrogel for Cell Printing

Extracellular matrix hydrogels from decellularized tissues: Structure and function

Homogenized Porcine Extracellular Matrix Derived Injectable Tissue Construct with Gold Nanoparticles for Musculoskeletal Tissue Engineering Applications

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