An injectable hyaluronic acid–tyramine hydrogel system for protein delivery

Lee, Fan; Chung, Joo Eun; Kurisawa, Motoichi

doi:10.1016/j.jconrel.2008.11.028

Cited by 282 publications

(220 citation statements)

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“…[1][2][3] These improvements make them easier to use, enable targeted delivery, [4][5][6] optimize delivery periods, minimize side effects by reducing drug dose, and increase patient comfort and compliance. 7 Creating in situ implants has become a frequently used technique, by which the drug and a biodegradable polymer are dissolved in an organic solvent and administered by subcutaneous injection, generating a semi-solid depot.…”

Section: Introductionmentioning

confidence: 99%

Application of hydroxyapatite nanoparticles in development of an enhanced formulation for delivering sustained release of triamcinolone acetonide

Koocheki

Madaeni²,

Niroomandi³

2011

IJN

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We report an analysis of in vitro and in vivo drug release from an in situ formulation consisting of triamcinolone acetonide (TR) and poly( d , l -lactide-co-glycolide) (PLGA) and the additives glycofurol (GL) and hydroxyapatite nanoparticles (HA). We found that these additives enhanced drug release rate. We used the Taguchi method to predict optimum formulation variables to minimize the initial burst. This method decreased the burst rate from 8% to 1.3%. PLGA-HA acted as a strong buffer, thereby preventing tissue inflammation at the injection site caused by the acidic degradation products of PLGA. Characterization of the optimized formulation by a variety of techniques, including scanning electron microscopy, X-ray diffraction, differential scanning calorimetry, and Fourier transform near infrared spectroscopy, revealed that the crystalline structure of TR was converted to an amorphous form. Therefore, this hydrophobic agent can serve as an additive to modify drug release rates. Data generated by in vitro and in vivo experiments were in good agreement.

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Section: Introductionmentioning

confidence: 99%

Application of hydroxyapatite nanoparticles in development of an enhanced formulation for delivering sustained release of triamcinolone acetonide

Koocheki

Madaeni²,

Niroomandi³

2011

IJN

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“…These values are higher than for pure gelatin with increased HA content but are still within the range of soft hydrogels. In another study with enzymatically crosslinked HA-Tyr hydrogels, Lee et al found that the increase in H 2 O 2 concentration produced an increase in its storage modulus, which has been correlated with the higher crosslinking density of the hydrogel when higher oxidation is achieved by the H 2 O 2 [134]. The reaction mechanism produced by the HRP/H 2 O 2 coupling starts with the oxidation of the HRP by the H 2 O 2 , which afterwards oxidizes the tyramine groups of the hyaluronic acid or the gelatin chain, see Figure 4-1 [134].…”

Section: Compression Testsmentioning

confidence: 98%

“…In another study with enzymatically crosslinked HA-Tyr hydrogels, Lee et al found that the increase in H 2 O 2 concentration produced an increase in its storage modulus, which has been correlated with the higher crosslinking density of the hydrogel when higher oxidation is achieved by the H 2 O 2 [134]. The reaction mechanism produced by the HRP/H 2 O 2 coupling starts with the oxidation of the HRP by the H 2 O 2 , which afterwards oxidizes the tyramine groups of the hyaluronic acid or the gelatin chain, see Figure 4-1 [134]. Then the percentage of tyramine groups forming crosslinking points will depend not only on the quantity of tyramine moieties grafted onto the gelatin or hyaluronic acid backbone, but also on the amount of H 2 O 2 .…”

Section: Compression Testsmentioning

confidence: 98%

“…Once the tyramine is grafted onto the polymeric chains, they can be crosslinked with horseradish peroxidase (HRP) and hydrogen peroxide (H 2 O 2 ), see Figure 1-10. H 2 O 2 acts as the oxidant of the tyramine molecule, and the HRP catalyzes the crosslinking reaction, oxidizing two tyramine molecules every crosslinking cycle [134]. Soybean peroxidase or hematin can be used as the catalysts instead of HRP [128], [135].…”

Section: Injectable Enzymatically Crosslinked Hydrogels Of Gelatin Anmentioning

confidence: 99%

“…In this reaction, the hydrogen peroxide acts as the oxidant of the HRP that then oxidizes two tyramines forming a covalent bond between them (see Figure 1 -10). After that, the HRP returns to its original state [134]. The crosslinking reaction is very fast, it takes less than one minute, avoiding the precipitation of the fibers in the gelatin solution and enabling homogeneous distribution of fibers in the hydrogel matrix.…”

Section: Morphology and Microstructure Of Compositesmentioning

confidence: 99%

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Protein-based injectable hydrogels towards the regeneration of articular cartilage

Poveda-Reyes¹

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A mis seres queridos, con ellos todo es posible "The future belongs to those who believe in the beauty of their dreams"Eleanor Roosevelt Acknowledgements 7 AcknowledgementsAfter reaching this point I would like to thank everybody that in one way or another has helped me through these four years of work.First of all, I gratefully acknowledge the financial support from the Spanish Ministry of Economy and Competitiveness through the DPI2010-20399-C04-03 and MAT2013-46467-C4-1-R projects, my FPI fellowship BES-2011-046144 and the EEBB-I-13-06179 and EEBB-I-14-08725 research stay grants.I would especially like to thank my thesis supervisor, Dr. Gloria Gallego Ferrer, for helping me in everything I needed through these four years, for giving me this opportunity and for all her efforts in my assistance. Also thanks to José Luis Gómez Ribelles for his valuable help and brilliant ideas.Thanks to the staff of the Microscopy Service at the Universitat Politècnica de València, for their guidance and help during the SEM sessions.I would also like to express my gratitude to my supervisors during my stays at other Research Centers. Thanks to Prof. Ulrica Edlund, who during my stay at the Fiber and Polymer Technology Department (KTH) in Stockholm, helped me with everything I could need and to all the people in the center (Laleh, Anas, Soheil, Bekir, Parmida, Robertus, Arturo, etc.) that helped me in the lab and made my stay easier. Thanks to Prof. Manuel Salmerón Sánchez at the Microenvironments for Medicine group (MiMe) (University of Glasgow) for giving me the opportunity to learn the basics of cell culture in his lab, his guidance and valuable help. I would also like to thank Dr. Vladimira Moulisova for teaching me everything I know of cell biology work and being so patient and friendly. And to all the people of the group (Marco, Sara, Eleni, Mark, Alex, Jake, Elie, Frankie, etc.), as they say "People make Glasgow" and they made me feel at home. I cannot forget all the people at the Center for Biomaterials and Tissue Engineering: to Laura Teruel, for being so patient with everyone, so helpful and keeping the lab in a perfect state, and all the lecturers for their help and advice, especially to Ana Vallés and Manuel Monleón, who introduced me to the wonderful field of Tissue Engineering.I would like to especially thank Tatiana C. Gamboa, who guided me in my first years in the lab. To Suhail, Rocío, Marta, Félix, Petra, Leonardo, Rebeca and Esther, I helped them in their first steps in the lab but I learnt a lot from them and they helped me a lot, both professional and personally. Also thanks to Alex Rodrigo who helped me a lot in my first cell cultures even if he had a million things to do.Thanks to all the people who have been in the "Sala de Reuniones" office: Manu, María, Line, Amparo G, Roberto, Guillermo, Álvaro, Laia, Rubén, etc., thanks for the break times, your laughs, your help and being the way you are.Special thanks to my friends, they gave me support through all these years, with them life is better.Finally, I w...

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Injectable in situ Gelling Hydrogels as Biomaterials

Singh

Nair

2012

Integrated Biomaterials for Biomedical Technology

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An injectable hyaluronic acid–tyramine hydrogel system for protein delivery

Cited by 282 publications

References 36 publications

Application of hydroxyapatite nanoparticles in development of an enhanced formulation for delivering sustained release of triamcinolone acetonide

Application of hydroxyapatite nanoparticles in development of an enhanced formulation for delivering sustained release of triamcinolone acetonide

Protein-based injectable hydrogels towards the regeneration of articular cartilage

Injectable in situ Gelling Hydrogels as Biomaterials

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