An innovative approach for the assessment of Bulgarian soybean cultivars

Abstract

The evaluation of climate plasticity and content of free amino acids, sugars and fatty acids in Bulgarian soybean cultivars were used as an innovative approach. The field performance, expression and metabolomic profiles of leaves, green seeds and mature seeds of plants grown from low temperature pre-treated and not-treated seeds were assessed by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and gas chromatography/mass spectrometry (GC/MS). The data from the morphological indicators, expression profiles and content of metabolites confirmed that the plants grown from low temperature pre-treated seeds had better performance. The expression profiles correlated with the content of amino acids and sugars. Inclusion of extruded full-fat soybean from cvs. Srebrina and Richy in the compound feed for pigs and soybean cake obtained after cold pressed soybeans of cv. Srebrina in compound feed for laying hens were investigated. The results confirmed that extruded full-fat soybean cv. Srebrina could be successfully included in the protein component of feed for growing pigs at a rate of 50% of soybean meal in protein equivalent. Extruded full-fat soybean cv. Richy in the amount of 30% of the protein component of the ration, in protein equivalent of the compound feed for the period of fattening of pigs could be included. The results confirmed that feed for laying hens, in which 50% of imported soybean meal is replaced by soybean cake produced from locally grown soybean, could increase the content of free amino and fatty acids of the final product and benefit the quality of eggs.

Transcriptional and Metabolic Profiling of Arabidopsis thaliana Transgenic Plants Expressing Histone Acetyltransferase HAC1 upon the Application of Abiotic Stress—Salt and Low Temperature

“…Polar and non-polar metabolites were determined by gas chromatography–mass spectrometry (GC–MS) in A. thaliana seedling samples from HAC OE and HAC RNAi lines with modified expression and control treated and non-treated with 150 mM NaCl at time points 0 h and 72 h. Samples in three replicates were analyzed as described earlier [ 37 , 38 ]. The identification of the metabolites was obtained by comparing the retention times and RI with those of authentic compounds and the spectral data obtained from the Golm Metabolome Database (GMD) [ 39 ] and the National Institute of Standards and Technology (NIST 08) libraries [ 40 ].…”

Wheat and Barley Grass Juice Addition to a Plant-Based Feed Improved Growth and Flesh Quality of Common Carp (Cyprinus carpio)

“…The polar (amino and organic acids, carbohydrates) and non-polar metabolites (saturated and unsaturated FA) were determined by gas chromatography-mass spectrometry (GC-MS) according to the method described previously [13]. The GC-MS analysis was carried out on a 7890A gas chromatograph (Agilent Technologies Inc., Santa Clara, San Francisco, CA, USA) interfaced with a 5975C mass selective detector (Agilent).…”