An innovative index to incorporate transcriptomic data into weight of evidence approaches for environmental risk assessment

Cecchetto, Martina; Peruzza, Luca; Giubilato, Elisa; Bernardini, Ilaria; Rovere, Giulia Dalla; Marcomini, Antonio; Regoli, Francesco; Bargelloni, Luca; Patarnello, Tomaso; Semenzin, Elena; Milan, Massimo

doi:10.1016/j.envres.2023.115745

Cited by 3 publications

(1 citation statement)

References 48 publications

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“…Evaluating potential threats linked to synergistic exposure effects (e.g., reduced dermal microbiota) or multiple exposure routes (i.e., aquatic and terrestrial) requires situation-specific ecological context. Implementation of weight of evidence effects could further classify cumulative threat levels of variable biomarker responses (Cecchetto et al, 2023). An initial step towards multi-stressor risk assessment is outlined here, namely by exploring stage-specific variance in biochemical pathways and identifying points of physiological vulnerability in the life cycle as a screening-level conservation approach.…”

Section: Discussionmentioning

confidence: 99%

Framework for multi-stressor physiological response evaluation in amphibian risk assessment and conservation

Awkerman,

Glinski,

Henderson

et al. 2024

Front. Ecol. Evol.

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Controlled laboratory experiments are often performed on amphibians to establish causality between stressor presence and an adverse outcome. However, in the field, identification of lab-generated biomarkers from single stressors and the interactions of multiple impacts are difficult to discern in an ecological context. The ubiquity of some pesticides and anthropogenic contaminants results in potentially cryptic sublethal effects or synergistic effects among multiple stressors. Although biochemical pathways regulating physiological responses to toxic stressors are often well-conserved among vertebrates, different exposure regimes and life stage vulnerabilities can yield variable ecological risk among species. Here we examine stress-related biomarkers, highlight endpoints commonly linked to apical effects, and discuss differences in ontogeny and ecology that could limit interpretation of biomarkers across species. Further we identify promising field-based physiological measures indicative of potential impacts to health and development of amphibians that could be useful to anuran conservation. We outline the physiological responses to common stressors in the context of altered functional pathways, presenting useful stage-specific endpoints for anuran species, and discussing multi-stressor vulnerability in the larger framework of amphibian life history and ecology. This overview identifies points of physiological, ecological, and demographic vulnerability to provide context in evaluating the multiple stressors impacting amphibian populations worldwide for strategic conservation planning.

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Section: Discussionmentioning

confidence: 99%