An innovative lattice Boltzmann model for simulating Michaelis–Menten-based diffusion–advection kinetics and its application within a cartilage cell bioreactor

Sayed, Abid A; Hussein, Mostafa; Becker, Thomas

doi:10.1007/s10237-009-0164-3

Cited by 11 publications

(7 citation statements)

References 25 publications

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“…Perfusion bioreactors are often simulated using generalized assumptions and simplifications; while these simpler models provide quick answers, they are limited to a maximum number of species and more over only provide an approximation of the operational parameters (Cioffi et al, 2006; Hidalgo‐Bastida et al, 2012; Okkels et al, 2011; Raimondi et al, 2006). Recently, new models for tissue engineering transport phenomena have been based in the lattice Boltzmann equation (LBE) technique, which is suited to parallel computation, multi‐scaling and to simple handling of complex boundary shaped scaffolds (Hussein et al, 2008; Moaty Sayed et al, 2010; Porter et al, 2005; Voronov et al, 2010; Youssef et al, 2012). LBE differs from conventional forms of computational fluid dynamics (CFD) by solving flow and CD at a micro‐scale.…”

Section: Introductionmentioning

confidence: 99%

In silico multi‐scale model of transport and dynamic seeding in a bone tissue engineering perfusion bioreactor

Spencer

Hidalgo‐Bastida

Cartmell

et al. 2012

Biotech & Bioengineering

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Computer simulations can potentially be used to design, predict, and inform properties for tissue engineering perfusion bioreactors. In this work, we investigate the flow properties that result from a particular poly-L-lactide porous scaffold and a particular choice of perfusion bioreactor vessel design used in bone tissue engineering. We also propose a model to investigate the dynamic seeding properties such as the homogeneity (or lack of) of the cellular distribution within the scaffold of the perfusion bioreactor: a pre-requisite for the subsequent successful uniform growth of a viable bone tissue engineered construct. Flows inside geometrically complex scaffolds have been investigated previously and results shown at these pore scales. Here, it is our aim to show accurately that through the use of modern high performance computers that the bioreactor device scale that encloses a scaffold can affect the flows and stresses within the pores throughout the scaffold which has implications for bioreactor design, control, and use. Central to this work is that the boundary conditions are derived from micro computed tomography scans of both a device chamber and scaffold in order to avoid generalizations and uncertainties. Dynamic seeding methods have also been shown to provide certain advantages over static seeding methods. We propose here a novel coupled model for dynamic seeding accounting for flow, species mass transport and cell advection-diffusion-attachment tuned for bone tissue engineering. The model highlights the timescale differences between different species suggesting that traditional homogeneous porous flow models of transport must be applied with caution to perfusion bioreactors. Our in silico data illustrate the extent to which these experiments have the potential to contribute to future design and development of large-scale bioreactors.

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Section: Introductionmentioning

confidence: 99%

In silico multi‐scale model of transport and dynamic seeding in a bone tissue engineering perfusion bioreactor

Spencer

Hidalgo‐Bastida

Cartmell

et al. 2012

Biotech & Bioengineering

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“…This cyclic flow field can be achieved by means of low frequency piezo elements embedded in the bioreactor, which can be a promising modification, but investigation on the presence of an electric field on the grown cells may be necessary. • Other ideas of pressurised moving flow field have shown promising result (Moaty et al 2009), this could be further investigated and applied on the scaffolds.…”

Section: Resultsmentioning

confidence: 97%

“…Along with the enhanced ability of complex geometry solutions, the method deals with the walls as a bounce back effect. Besides, the ability to fully model different chemical reactions and kinetics especially cell activity was as well achieved (Hussein 2006;Hussein et al 2008a, b;Moaty et al 2009). In consequence, LBM has proven relieving all sophistications in modelling such complicated biotechnological aspects.…”

Section: Introductionmentioning

confidence: 97%

Numerical modelling of shear and normal stress of micro-porous ceramics for stimulated in-vitro cultivation of bone cells

Hussein

Becker

2009

Microfluid Nanofluid

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Tissue engineering is a multidisciplinary job and has been of great challenge to scientist since its emerge in the 1980s. Special problems arise in this science, since bone cells are usually growing inside humans under moderate cyclic mechanical loadings (0.66-1.5 MPa); it is expected to grow them in a similar background. An innovative contribution is made where the shear stress is modelled inside in micro-channels reporting the various important design considerations of scaffolds, providing a more appropriate understanding of geometry change versus shear stress and Darcy's Reynolds number in micro-channels. The geometry of the scaffolds was captured by analysing and processing batches of images with edge detection techniques which mainly works on the colour contrasts. A novel edge detection algorithm which spots the colour change by means of gradient calculations was successful to scan the Micro-CT (computer tomography) images and gather a binary file for the 3-D scaffolds of 430 sections for a 3 mm. The 3-D Lattice Boltzmann was successful in modelling the transport phenomena and reporting the shear stress distribution in straight microchannels and in the complicated micro-scaffolds. An analogical study is as well made between the realistic stresses expected in humans and the simulated ones, reporting different discussion aspects to bring the designed structures to a more realistic stage. Using micro-porous scaffolds have proven much more realistic in imitating the human bone shear load conditions (0.1 MPa) with at least two orders of magnitude closer (5.3 9 10 -4 MPa) than previously published results (1.09 9 10 Pa). Further study should be done to realise closer shear loads to measured data in humans.

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“…4 Several other bioreactor models have been already characterized by CFD modeling of internal flow and pressure distribution. 11,26,28,34,49–51 In our model, CFD simulation helped define the optimal design for laminar flow perfusion with concomitant pressure regulation via an internal iris system. To our knowledge, this approach is unique compared with existing perfusion bioreactor models.…”

Section: Discussionmentioning

confidence: 99%

“…23,25–28 Most of these models have focused on describing the flow through porous scaffolds using various approaches, without taking the shape of the reactor itself into account. 23,27,29–34 Considering the bioreactor shape in the modeling approach, a recent study suggested a quadrangular design being superior to the frequently used cylindrical design for improved scaffold perfusion.…”

Section: Introductionmentioning

confidence: 99%

A Differential Pressure Laminar Flow Reactor Supports Osteogenic Differentiation and Extracellular Matrix Formation from Adipose Mesenchymal Stem Cells in a Macroporous Ceramic Scaffold

Weyand

Kasper

Israelowitz³

et al. 2012

BioResearch Open Access

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We present a laminar flow reactor for bone tissue engineering that was developed based on a computational fluid dynamics model. The bioreactor design permits a laminar flow field through its specific internal shape. An integrated bypass system that prevents pressure build-up through bypass openings for pressure release allows for a constant pressure environment during the changing of permeability values that are caused by cellular growth within a porous scaffold. A macroporous ceramic scaffold, composed of zirconium dioxide, was used as a test biomaterial that studies adipose stem cell behavior within a controlled three-dimensional (3D) flow and pressure environment. The topographic structure of the material provided a basis for stem cell proliferation and differentiation toward the osteogenic lineage. Dynamic culture conditions in the bioreactor supported cell viability during long-term culture and induced cell cluster formation and extra-cellular matrix deposition within the porous scaffold, though no complete closure of the pores with new-formed tissue was observed. We postulate that our system is suitable for studying fluid shear stress effects on stem cell proliferation and differentiation toward bone formation in tissue-engineered 3D constructs.

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An innovative lattice Boltzmann model for simulating Michaelis–Menten-based diffusion–advection kinetics and its application within a cartilage cell bioreactor

Cited by 11 publications

References 25 publications

In silico multi‐scale model of transport and dynamic seeding in a bone tissue engineering perfusion bioreactor

In silico multi‐scale model of transport and dynamic seeding in a bone tissue engineering perfusion bioreactor

Numerical modelling of shear and normal stress of micro-porous ceramics for stimulated in-vitro cultivation of bone cells

A Differential Pressure Laminar Flow Reactor Supports Osteogenic Differentiation and Extracellular Matrix Formation from Adipose Mesenchymal Stem Cells in a Macroporous Ceramic Scaffold

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