An insecticide phenotypic resistance diagnostic kit for cotton aphid Aphis gossypii Glover (Hemiptera Aphididae)

Abstract

Background: Aphis gossypii is a notorious pest worldwide, and evidence of resistance of A. gossypii to various insecticides has been documented. Diagnostic tools for the rapid and accurate assessment of insecticide resistance are urgently needed to implement effective pest control and insecticide resistance management strategies.Results: Using this diagnostic kit based on the glass vial bioassay, detection results can be obtained in 3 h and the values of 897.86, 133.57, 12 037.45, 2849.26, 19 457.33 and 215.60 ng/cm 2 were finally identified as the actual diagnostic doses of imidacloprid, acetamiprid, thiamethoxam, nitenpyram, dinotefuran and sulfoxaflor, respectively. The regression equation between mortalities under diagnostic doses and actual resistance ratios tested by the leaf-dipping method were conducted in different strains of A. gossypii, and the diagnostic mortality of A. gossypii was negatively correlated with the resistance ratio to imidacloprid (r = −0.986, P = 0.002), acetamiprid (r = −0.964, P = 0.008), thiamethoxam (r = −0.930, P = 0.022), nitenpyram (r = −0.950, P = 0.013), dinotefuran (r = −0.976, P = 0.004) and sulfoxaflor (r = −0.937, P = 0.019). Moreover, four A. gossypii field populations were selected to apply the diagnostic kit in the field.Conclusions: A diagnostic kit based on the glass vial bioassay for the rapid detection of resistance to imidacloprid, acetamiprid, thiamethoxam, nitenpyram, dinotefuran and sulfoxaflor in A. gossypii was developed. The insecticide diagnostic kit for A. gossypii can be a useful screening tool to determine effective insecticides quickly and accurately.

UDP-Glycosyltransferases UGT350C3 and UGT344L7 Confer Tolerance to Neonicotinoids in Field Populations of Aphis gossypii

“…1,2 At present, the use of chemical insecticides is the most effective method for controlling A. gossypii. However, the cotton aphid has developed different levels of resistance to various insecticides, including carbamates, 3 diamides, 4 pyrethroids, 5 organophosphates, 6 sulfoxaflor, 7 and neonicotinoids, 6,8 due to the unscientific use of insecticides in the field. Neonicotinoids, as broad spectrum insecticides, are widely valued because of their marked ability to control sucking and chewing insects and their lower toxicity to nontarget organisms, 9,10 as they bind to nicotinic acetylcholine receptor (nAChR), leading to nerve stimulation, paralysis, and death in insects.…”

Rapid test to detect insecticide resistance in field populations of Spodoptera frugiperda (Lepidoptera: Noctuidae)

“…Previous research has developed a diagnostic kit for the rapid detection of resistance to imidacloprid, nitenpyram, clothianidin, dinotefuran, thiamethoxam, isoprocarb and chlorpyrifos in Sogatella furcifera based on the glass vial bioassay ( Mao et al, 2021 ). Huang et al (2022) developed an insecticide phenotypic resistance diagnostic kit for Aphis gossypii , where the results can be obtained within 3 h. These successful applications show that an efficient and rapid method of detecting the resistance levels of S. frugiperda to insecticides will be possible.…”