An Insight into Efflux-Mediated Arsenic Resistance and Biotransformation Potential of Enterobacter Cloacae RSC3 from Arsenic Polluted Area

Bhati, Reeta; Sreedharan, Smitha Mony; Rizvi, Asfa; Khan, Mohammad Saghir; Singh, Rajni

doi:10.1007/s12088-022-01028-7

Cited by 8 publications

(4 citation statements)

References 34 publications

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“…ISTPL4, there was no change in color observed but the findings of whole genome sequencing indicated the presence of arsenic-resistant genes in Z. sp. ISTPL4 [ 18 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…Spot inoculation of Z. sp. ISTPL4 was conducted, and the bacterial culture was kept for incubation at 37 °C for 72 h. After fungal and bacterial growth, 100 µL AgNO 3 (0.1 M) solution was flooded over the grown fungal and bacterial plates and a change in color was observed [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…For this, nutrient agar was supplemented with 500 ppm of As (V) and the fungal disc was inoculated followed by an incubation of 15 days at 28 ± 2 • C. Spot inoculation of Z. sp. ISTPL4 was conducted, and the bacterial culture was kept for incubation at 37 • C for 72 h. After fungal and bacterial growth, 100 µL AgNO 3 (0.1 M) solution was flooded over the grown fungal and bacterial plates and a change in color was observed [18].…”

Section: Biotransformation Of Arsenicmentioning

confidence: 99%

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Fungal-Bacterial Combinations in Plant Health under Stress: Physiological and Biochemical Characteristics of the Filamentous Fungus Serendipita indica and the Actinobacterium Zhihengliuella sp. ISTPL4 under In Vitro Arsenic Stress

Sharma,

Koul,

Joshi

et al. 2024

Microorganisms

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Fungal-bacterial combinations have a significant role in increasing and improving plant health under various stress conditions. Metabolites secreted by fungi and bacteria play an important role in this process. Our study emphasizes the significance of secondary metabolites secreted by the fungus Serendipita indica alone and by an actinobacterium Zhihengliuella sp. ISTPL4 under normal growth conditions and arsenic (As) stress condition. Here, we evaluated the arsenic tolerance ability of S. indica alone and in combination with Z. sp. ISTPL4 under in vitro conditions. The growth of S. indica and Z. sp. ISTPL4 was measured in varying concentrations of arsenic and the effect of arsenic on spore size and morphology of S. indica was determined using confocal microscopy and scanning electron microscopy. The metabolomics study indicated that S. indica alone in normal growth conditions and under As stress released pentadecanoic acid, glycerol tricaprylate, L-proline and cyclo(L-prolyl-L-valine). Similarly, d-Ribose, 2-deoxy-bis(thioheptyl)-dithioacetal were secreted by a combination of S. indica and Z. sp. ISTPL4. Confocal studies revealed that spore size of S. indica decreased by 18% at 1.9 mM and by 15% when in combination with Z. sp. ISTPL4 at a 2.4 mM concentration of As. Arsenic above this concentration resulted in spore degeneration and hyphae fragmentation. Scanning electron microscopy (SEM) results indicated an increased spore size of S. indica in the presence of Z. sp. ISTPL4 (18 ± 0.75 µm) compared to S. indica alone (14 ± 0.24 µm) under normal growth conditions. Our study concluded that the suggested combination of microbial consortium can be used to increase sustainable agriculture by combating biotic as well as abiotic stress. This is because the metabolites released by the microbial combination display antifungal and antibacterial properties. The metabolites, besides evading stress, also confer other survival strategies. Therefore, the choice of consortia and combination partners is important and can help in developing strategies for coping with As stress.

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“…ISTPL4, there was no change in color observed but the findings of whole genome sequencing indicated the presence of arsenic-resistant genes in Z. sp. ISTPL4 [ 18 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Biotransformation Of Arsenicmentioning

confidence: 99%

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Fungal-Bacterial Combinations in Plant Health under Stress: Physiological and Biochemical Characteristics of the Filamentous Fungus Serendipita indica and the Actinobacterium Zhihengliuella sp. ISTPL4 under In Vitro Arsenic Stress

Sharma,

Koul,

Joshi

et al. 2024

Microorganisms

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“…Also, manipulations of genes can increase siderophore, metallothionine production, and exo-polymer production increasing mitigation of heavy metals. In addition to this, efficient indigenous microbial culture can be used to achieve the desired bioremediation of wastewaters and soil environment [99].…”

Section: Microbiome Engineeringmentioning

confidence: 99%

Novel Approaches for Sustainable Management of Chromium Contaminated Wastewater

Kumar,

Singh Saini

2024

Wastewater Treatment - Past and Future Perspectives [Working Title]

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The release of high volumes of untreated effluents containing different forms of chromium into waterbodies and further use of this wastewater for ferti-irrigation purposes pose a direct threat to health of human populations consuming produces from such agricultural fields. The higher concentration of chromium above permissible limits at these sites may pose harm to flora and fauna. The conventional processes used for treatment of chromium-containing effluents have low treatment efficiency, high operational costs, and produce toxic sludge requiring safe disposal. In contrast, the approaches exploiting use of living systems, such as microbes/microbial products and microbes, may provide sustainable treatment options. The emerging advanced/novel treatment technologies based on harnessing metabolic potential of microbiome of the polluted sites have potential to achieve the efficient removal of heavy metals from polluted sites. The success of protocols developed and tested at lab scale needs to be replicated at pilot/industrial to handle high volumes with varying levels of organic co-contaminants and harsh physiological conditions. The presented chapter provides an overview of impact of high chromium levels on ecosystem and various treatment processes with advanced aspect of management of heavy metals to prevent harmful effects on the environment.

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Hybrid-genome sequence analysis of Enterobacter cloacae FACU and morphological characterization: insights into a highly arsenic-resistant strain

Abdelhadi,

Elarabi,

Ibrahim

et al. 2024

Funct Integr Genomics

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An Insight into Efflux-Mediated Arsenic Resistance and Biotransformation Potential of Enterobacter Cloacae RSC3 from Arsenic Polluted Area

Cited by 8 publications

References 34 publications

Fungal-Bacterial Combinations in Plant Health under Stress: Physiological and Biochemical Characteristics of the Filamentous Fungus Serendipita indica and the Actinobacterium Zhihengliuella sp. ISTPL4 under In Vitro Arsenic Stress

Fungal-Bacterial Combinations in Plant Health under Stress: Physiological and Biochemical Characteristics of the Filamentous Fungus Serendipita indica and the Actinobacterium Zhihengliuella sp. ISTPL4 under In Vitro Arsenic Stress

Novel Approaches for Sustainable Management of Chromium Contaminated Wastewater

Hybrid-genome sequence analysis of Enterobacter cloacae FACU and morphological characterization: insights into a highly arsenic-resistant strain

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