An insight into the gene-networks playing a crucial role in the cotton plant architecture regulation

Naveed, Salman; Jones, Michael; Campbell, Todd; Rustgi, Sachin

doi:10.1007/s13237-023-00446-2

Cited by 4 publications

(3 citation statements)

References 79 publications

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“…Similarly, overexpression of AtCKX1 and AtCKX3 in transgenic plants resulted in reduced inflorescence size and reduced ability to form floral primordia compared to wild-type plants ( Werner et al, 2003 ). In cotton, recent studies have begun to uncover the multiple roles of CKX genes in physiological processes such as leaf defoliation, fruit branch internode elongation and stress responses ( Naveed et al, 2023 ). Using comprehensive analyses such as RNA-Seq techniques, various studies have shown that CKX genes are upregulated in response to treatments with abscisic acid (ABA) and thidiazuron (TDZ), indicating their involvement in cotton leaf defoliation ( Zhou et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Genomic insights into CKX genes: key players in cotton fibre development and abiotic stress responses

Hamid,

Jacob,

Ghorbanzadeh

et al. 2024

PeerJ

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Cytokinin oxidase/dehydrogenase (CKX), responsible for irreversible cytokinin degradation, also controls plant growth and development and response to abiotic stress. While the CKX gene has been studied in other plants extensively, its function in cotton is still unknown. Therefore, a genome-wide study to identify the CKX gene family in the four cotton species was conducted using transcriptomics, quantitative real-time PCR (qRT-PCR) and bioinformatics. As a result, in G. hirsutum and G. barbadense (the tetraploid cotton species), 87 and 96 CKX genes respectively and 62 genes each in G. arboreum and G. raimondii, were identified. Based on the evolutionary studies, the cotton CKX gene family has been divided into five distinct subfamilies. It was observed that CKX genes in cotton have conserved sequence logos and gene family expansion was due to segmental duplication or whole genome duplication (WGD). Collinearity and multiple synteny studies showed an expansion of gene families during evolution and purifying selection pressure has been exerted. G. hirsutum CKX genes displayed multiple exons/introns, uneven chromosomal distribution, conserved protein motifs, and cis-elements related to growth and stress in their promoter regions. Cis-elements related to resistance, physiological metabolism and hormonal regulation were identified within the promoter regions of the CKX genes. Expression analysis under different stress conditions (cold, heat, drought and salt) revealed different expression patterns in the different tissues. Through virus-induced gene silencing (VIGS), the GhCKX34A gene was found to improve cold resistance by modulating antioxidant-related activity. Since GhCKX29A is highly expressed during fibre development, we hypothesize that the increased expression of GhCKX29A in fibres has significant effects on fibre elongation. Consequently, these results contribute to our understanding of the involvement of GhCKXs in both fibre development and response to abiotic stress.

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Section: Introductionmentioning

confidence: 99%

Genomic insights into CKX genes: key players in cotton fibre development and abiotic stress responses

Hamid,

Jacob,

Ghorbanzadeh

et al. 2024

PeerJ

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“…One of the members of this gene family plays an important role in promoting the determinate growth habit in plants by serving as a key component of the florigen activation complex (FAC), which activates the expression of downstream meristem identity genes SUPPRES-SOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1), LEAFY (LFY), APETALA 1 (AP1), and FRUITFUL (FUL). On the other hand, reduced expression of the cotton FT, SOC1, and FUL genes supports vegetative growth, delayed flowering, and bushy architecture in cot-ton [35]. The development of cotton cultivars with somewhat annual growth habit having fewer vegetative branches and reduced regrowth after defoliation, which are desirable traits in cotton, has led to compact plants with reduced vegetative and more reproductive growth with improved yield under optimal growth conditions, which facilitate mechanical picking [35][36][37].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, reduced expression of the cotton FT, SOC1, and FUL genes supports vegetative growth, delayed flowering, and bushy architecture in cot-ton [35]. The development of cotton cultivars with somewhat annual growth habit having fewer vegetative branches and reduced regrowth after defoliation, which are desirable traits in cotton, has led to compact plants with reduced vegetative and more reproductive growth with improved yield under optimal growth conditions, which facilitate mechanical picking [35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Development of High-Yielding Upland Cotton Genotypes with Reduced Regrowth after Defoliation Using a Combination of Molecular and Conventional Approaches

Naveed,

Toyinbo,

Ingole

et al. 2023

Genes

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Cotton is an economically important crop. However, the yield gain in cotton has stagnated over the years, probably due to its narrow genetic base. The introgression of beneficial variations through conventional and molecular approaches has helped broaden its genetic base to some extent. The growth habit of cotton is one of the crucial factors that determine crop maturation time, yield, and management. This study used 44 diverse upland cotton genotypes to develop high-yielding cotton germplasm with reduced regrowth after defoliation and early maturity by altering its growth habit from perennial to somewhat annual. We selected eight top-scoring genotypes based on the gene expression analysis of five floral induction and meristem identity genes (FT, SOC1, LFY, FUL, and AP1) and used them to make a total of 587 genetic crosses in 30 different combinations of these genotypes. High-performance progeny lines were selected based on the phenotypic data on plant height, flower and boll numbers per plant, boll opening date, floral clustering, and regrowth after defoliation as surrogates of annual growth habit, collected over four years (2019 to 2022). Of the selected lines, 8×5-B3, 8×5-B4, 9×5-C1, 8×9-E2, 8×9-E3, and 39×5-H1 showed early maturity, and 20×37-K1, 20×37-K2, and 20×37-D1 showed clustered flowering, reduced regrowth, high quality of fiber, and high lint yield. In 2022, 15 advanced lines (F8/F7) from seven cross combinations were selected and sent for an increase to a Costa Rica winter nursery to be used in advanced testing and for release as germplasm lines. In addition to these breeding lines, we developed molecular resources to breed for reduced regrowth after defoliation and improved yield by converting eight expression-trait-associated SNP markers we identified earlier into a user-friendly allele-specific PCR-based assay and tested them on eight parental genotypes and an F2 population.

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Plant cytogenetics blurring disciplinary boundaries to sustain global food security

Rustgi

2023

Nucleus

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An insight into the gene-networks playing a crucial role in the cotton plant architecture regulation

Cited by 4 publications

References 79 publications

Genomic insights into CKX genes: key players in cotton fibre development and abiotic stress responses

Genomic insights into CKX genes: key players in cotton fibre development and abiotic stress responses

Development of High-Yielding Upland Cotton Genotypes with Reduced Regrowth after Defoliation Using a Combination of Molecular and Conventional Approaches

Plant cytogenetics blurring disciplinary boundaries to sustain global food security

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