An insight into the molecular characteristics of hepatitis C virus for clinicians

Du, Lingyao; Tang, Hong

doi:10.15537/smj.2016.5.14178

Cited by 7 publications

(2 citation statements)

References 63 publications

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“…In contrast, in our study reduced plasma levels of cholesterol were significantly associated with chronic HEV infection in the tacrolimus-treated group. It can be explained by the evident abnormal fatty deposit (macro and micro steatosis) in liver parenchyma [ 44 ], as observed in our study (G2 animals). Regarding haematological features, the WBC count was not reduced by tacrolimus treatment, as expected [ 45 ] and platelets account could not be associated with HEV chronicity, as previously reported in SOT patients infected with HEV [ 19 ].…”

Section: Discussionsupporting

confidence: 61%

Cynomolgus monkeys are successfully and persistently infected with hepatitis E virus genotype 3 (HEV-3) after long-term immunosuppressive therapy

et al. 2017

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Epidemiological studies found that hepatitis E virus genotype 3 (HEV-3) infection was associated with chronic hepatitis and cirrhosis in immunocompromised patients. Our study aimed to investigate the relationship between the host immunosuppressive status and the occurrence of HEV-related chronic hepatitis. Here we describe a successful experimental study, using cynomolgus monkeys previously treated with tacrolimus, a potent calcineurin inhibitor immunosuppressant, and infected with a Brazilian HEV-3 strain isolated from naturally infected pigs. HEV infected monkeys were followed up during 160 days post infection (dpi) by clinical signs; virological, biochemical and haematological parameters; and liver histopathology. The tacrolimus blood levels were monitored throughout the experiment. Immunosuppression was confirmed by clinical and laboratorial findings, such as: moderate weight loss, alopecia, and herpes virus opportunistic infection. In this study, chronic HEV infection was characterized by the mild increase of liver enzymes serum levels; persistent RNA viremia and viral faecal shedding; and liver histopathology. Three out of four immunosuppressed monkeys showed recurrent HEV RNA detection in liver samples, evident hepatocellular ballooning degeneration, mild to severe macro and microvesicular steatosis (zone 1), scattered hepatocellular apoptosis, and lobular focal inflammation. At 69 dpi, liver biopsies of all infected monkeys revealed evident ballooning degeneration (zone 3), discrete hepatocellular apoptosis, and at most mild portal and intra-acinar focal inflammation. At 160 dpi, the three chronically HEV infected monkeys showed microscopic features (piecemeal necrosis) corresponding to chronic hepatitis in absence of fibrosis and cirrhosis in liver parenchyma. Within 4-months follow up, the tacrolimus-immunosuppressed cynomolgus monkeys infected with a Brazilian swine HEV-3 strain exhibited more severe hepatic lesions progressing to chronic hepatitis without liver fibrosis, similarly as shown in tacrolimus-immunosuppressed solid organ transplant (SOT) recipients. The cause-effect relationship between HEV infection and tacrolimus treatment was confirmed in this experiment.

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Section: Discussionsupporting

confidence: 61%

Cynomolgus monkeys are successfully and persistently infected with hepatitis E virus genotype 3 (HEV-3) after long-term immunosuppressive therapy

et al. 2017

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“…ORF is divided into structural genes (C, E1, and E2) and nonstructural genes (NS2, NS3, NS4a, NS4b, NS5a, and NS5b). The C gene encodes nucleocapsid protein; E1 and E2 genes encode glycosylated proteins of the envelope membrane; nonstructural genes encode nonstructural proteins, which participate in the processes of virus replication [ 2 ]. According to the level of nucleotide sequence homology, HCV isolates are divided into seven genotypes.…”

Section: Introductionmentioning

confidence: 99%

The Impact of Hepatitis C Virus Genotypes on Oxidative Stress Markers and Catalase Activity

Đorđević

Kocić

et al. 2021

Oxidative Medicine and Cellular Longevity

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Hepatitis C virus (HCV) is a major cause of liver disease worldwide. Chronic HCV infections are usually associated with increased oxidative stress in the liver tissue. The intensity of oxidative stress may be a detrimental factor in liver injury and may determine the severity of the disease. The aim of the present case-control study was to determine the level of lipid peroxidation (TBARS), protein oxidative modification (AOPP), and catalase activity in sera of patients infected with HCV, in relation to different HCV genotypes and viral load. Considering the HCV patients with chronic hepatitis C (52) and control subject (50) recruitment, the study was designed as a case–control-type. The HCV RNA isolation, viral load, and HCV genotyping were performed according to the standard protocols. A significant difference compared to control healthy subjects was reported for TBAR ( p < 0.001 ), AOPP ( p = 0.001 ), and catalase activity ( p = 0.007 ). In a gender-based comparison, a significantly higher level of AOPP for females was reported ( p < 0.001 ). As stratified by HCV genotype, the most common was HCV-1 (HCV-1a and HCV 1b), with the overall participation of more than 60%, followed by genotype 3, while the least represented was genotype 2. No significant difference was documented among genotypes in regard to oxidative stress markers, although somewhat higher TBARS level, but not significant, was registered in patients infected with genotype 1b. A statistically significant positive correlation was found between the concentration of HCV genome copies and AOPP ( r = 0.344 ; p = 0.012 ). A high level of HCV viral load was more likely to have a higher TBARS, but still without statistical significance ( p = 0.072 ). In conclusion, the results obtained confirmed an imbalance between the ROS production and antioxidative defense system in HCV-infected patients. Since oxidative stress may have a profound influence on disease progression, fibrosis, and carcinogenesis, our results may meet the aspirations of mandatory introduction of antioxidants as early HCV therapy to counteract ROS consequences.

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A therapeutic approach for the hepatitis C virus: in silico design of an antisense oligonucleotide-based candidate capsid inhibitor

Hasturk,

Eren

2024

Virus Genes

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An insight into the molecular characteristics of hepatitis C virus for clinicians

Cited by 7 publications

References 63 publications

Cynomolgus monkeys are successfully and persistently infected with hepatitis E virus genotype 3 (HEV-3) after long-term immunosuppressive therapy

Cynomolgus monkeys are successfully and persistently infected with hepatitis E virus genotype 3 (HEV-3) after long-term immunosuppressive therapy

The Impact of Hepatitis C Virus Genotypes on Oxidative Stress Markers and Catalase Activity

A therapeutic approach for the hepatitis C virus: in silico design of an antisense oligonucleotide-based candidate capsid inhibitor

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