An insight into the salivary transcriptome and proteome of the adult female mosquito Culex pipiens quinquefasciatus

Ribeiro, José M. C.; Charlab, Rosane; Pham, Van My; Garfield, Mark; Valenzuela, Jesús G.

doi:10.1016/j.ibmb.2004.02.008

Cited by 153 publications

(179 citation statements)

References 58 publications

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“…amboinensis were further characterized into 14 subgroups according to function (Table 1). As observed in previous sialotranscriptomes (Francischetti et al, 2002;Ribeiro et al, 2004a;Ribeiro et al, 2004b), the two larger sets were associated with protein synthesis machinery (45 EST in 26 clusters) and with energy metabolism (4 clusters containing 4 EST). Additional inspection of each cluster for further information can be done online with Supplemental Table S1.…”

Section: H Genessupporting

confidence: 76%

“…aegypti), and an endonuclease (2 EST in 1 cluster). All these types of transcripts were previously found in all mosquito sialotranscriptomes done to date, except for the endonuclease, which was found only on in those of Culex pipiens quinquefasciatus (Ribeiro et al, 2004b), where the secreted recombinant enzyme was studied (Calvo and Ribeiro, 2006), and Phlebotomines (Anderson et al, 2006;Oliveira et al, 2006). Transcripts coding for the other enzymes are most probably associated with sugar feeding and antimicrobial activity.…”

Section: Putative Secreted Proteins Belonging To Ubiquitous Protein Fmentioning

confidence: 91%

“…For most of these putative secreted polypeptides, no functional assignment is possible based on their primary sequence. Many of these proteins are unique to mosquitoes, and some are unique to either Culicines or Anophelines (Arca et al, 2005;Calvo et al, 2004;Ribeiro et al, 2004b;Valenzuela et al, 2003). In an attempt to separate secreted salivary gene products into those assisting either blood or sugar feeding, quantitative polymerase chain reaction (PCR) or DNA microarray analysis of different tissues and sexes have been attempted for Anopheles gambiae (Arca et al, 2005), AEdes aegypti , and AEdes albopictus , as well as a comparative analysis of male and female sialotranscriptomes, in the case of An.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An insight into the sialotranscriptome of the non-blood feeding Toxorhynchites amboinensis mosquito

Calvo

Pham

Ribeiro

2008

Insect Biochemistry and Molecular Biology

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All adult mosquitoes take sugar meals, and most adult females also take blood meals to develop eggs. Salivary glands (SG) of males are thus much smaller and do not contain many of the antihemostatic and antiinflammatory compounds found in females. In the past 5 years, transcriptome analyses have identified nearly 70 different genes expressed in adult female SG. For most of these, no function can be assigned in either blood or sugar feeding. Exceptionally, Toxorhynchites mosquitoes are unusual in that they never feed on blood, and the SG of adults are identical in both sexes. Transcriptome analysis of the adult SG of this mosquito was performed to increase knowledge of the evolution of blood feeding-and to identify polypeptide families associated with sugar feeding-in mosquitoes.

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Section: H Genessupporting

confidence: 76%

Section: Putative Secreted Proteins Belonging To Ubiquitous Protein Fmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An insight into the sialotranscriptome of the non-blood feeding Toxorhynchites amboinensis mosquito

Calvo

Pham

Ribeiro

2008

Insect Biochemistry and Molecular Biology

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“…darlingi 4 , Cx. quinquefasciatus 36 , and Ixodes scapularis 46 . Recent improvements in 2D-PAGE, mass spectrometry, and bioinformatics provide additional tools to characterize small amounts of protein.…”

Section: Discussionmentioning

confidence: 99%

Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)

Jariyapan

Choochote

Jitpakdi

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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SUMMARYSalivary gland proteins of the human malaria vector, Anopheles dirus B were determined and analyzed. The amount of salivary gland proteins in mosquitoes aged between 3 -10 days was approximately 1.08 ± 0.04 µg/female and 0.1 ± 0.05 µg/male. The salivary glands of both sexes displayed the same morphological organization as that of other anopheline mosquitoes. In females, apyrase accumulated in the distal regions, whereas alpha-glucosidase was found in the proximal region of the lateral lobes. This differential distribution of the analyzed enzymes reflects specialization of different regions for sugar and blood feeding. SDS-PAGE analysis revealed that at least seven major proteins were found in the female salivary glands, of which each morphological region contained different major proteins. Similar electrophoretic protein profiles were detected comparing unfed and blood-fed mosquitoes, suggesting that there is no specific protein induced by blood. Two-dimensional polyacrylamide gel analysis showed the most abundant salivary gland protein, with a molecular mass of approximately 35 kilodaltons and an isoelectric point of approximately 4.0. These results provide basic information that would lead to further study on the role of salivary proteins of An. dirus B in disease transmission and hematophagy.

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“…aegypti, calreticulins, fibrinogen/fibronectins, serpins, phosphatidylethanolamine-binding proteins, odorant-binding proteins, alkaline phosphatases, lectins, and hydrolases are expressed in both the male AG/ED and the female salivary glands (Valenzuela et al, 2002;. Genes encoding proteins in these classes are also expressed in the salivary glands and digestive tracts of other blood-feeding arthropods (Jaworski et al, 1995;Ribeiro et al, 2004;Arca et al, 2005;Andersen et al, 2007).…”

Section: 5g Novel Proteins-eight Of the Putativementioning

confidence: 99%

Identity and transfer of male reproductive gland proteins of the dengue vector mosquito, Aedes aegypti: Potential tools for control of female feeding and reproduction

Sirot

Poulson

McKenna

et al. 2008

Insect Biochemistry and Molecular Biology

155

166

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Male reproductive gland proteins (mRGPs) impact the physiology and/or behavior of mated females in a broad range of organisms. We sought to identify mRGPs of the yellow fever mosquito, Aedes aegypti, the primary vector of dengue and yellow fever viruses. Earlier studies with Ae. aegypti demonstrated that "matrone" (a partially purified male reproductive accessory gland substance) or male accessory gland fluid injected into virgin female Ae. aegypti affect female sexual refractoriness, blood feeding and digestion, flight, ovarian development, and oviposition. Using bioinformatic comparisons to Drosophila melanogaster accessory gland proteins and mass spectrometry of proteins from Ae. aegypti male accessory glands and ejaculatory ducts (AG/ED) and female reproductive tracts, we identified 63 new putative Ae. aegypti mRGPs. Twenty-one of these proteins were found in the reproductive tract of mated females, but not of virgin females, suggesting that they are transferred from males to females during mating. Most of the putative mRGPs fall into the same protein classes as mRGPs in other organisms, although some appear to be evolving rapidly and lack identifiable homologs in Culex pipiens, Anopheles gambiae, and D. melanogaster. Our results identify candidate male-derived molecules that may have an important influence on behavior, survival and reproduction of female mosquitoes.

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An insight into the salivary transcriptome and proteome of the adult female mosquito Culex pipiens quinquefasciatus

Cited by 153 publications

References 58 publications

An insight into the sialotranscriptome of the non-blood feeding Toxorhynchites amboinensis mosquito

An insight into the sialotranscriptome of the non-blood feeding Toxorhynchites amboinensis mosquito

Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)

Identity and transfer of male reproductive gland proteins of the dengue vector mosquito, Aedes aegypti: Potential tools for control of female feeding and reproduction

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