An insight into the transcriptome and proteome of the salivary gland of the stable fly, Stomoxys calcitrans

Wang, Xuyong; Ribeiro, José M. C.; Broce, Alberto B.; Wilkerson, Melinda J.; Kanost, Michael R.

doi:10.1016/j.ibmb.2009.06.004

Cited by 34 publications

(37 citation statements)

References 63 publications

(78 reference statements)

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“…The stable fly salivary gland transcriptome was recently reported (Wang et al, 2009) and the sequencing effort presented here represents a valuable database of genes expressed throughout stable Figure 9. Analysis of six putative stable fly CSP transcripts by RT-PCR.…”

Section: Discussionmentioning

confidence: 99%

“…Expressed sequence tag (EST) libraries are routinely developed for the identification of targets with relevance for use in insect control technologies (Gomulski et al, 2008;Guerrero et al, 2009;Hunter et al, 2009), but such genomic resources for the stable fly are limited (Wang et al, 2009). The current study describes the development of a database of genes expressed by the stable fly at both the immature and adult stages using pyrosequencing and traditional Sanger sequencing.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of expressed sequence tags from a significant livestock pest, the stable fly (Stomoxys calcitrans), identifies transcripts with a putative role in chemosensation and sex determination

Olafson

Lohmeyer

Dowd

2010

Arch Insect Biochem Physiol

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The stable fly, Stomoxys calcitrans L. (Diptera: Muscidae), is one of the most significant pests of livestock in the United States. The identification of targets for the development of novel control for this pest species, focusing on those molecules that play a role in successful feeding and reproduction, is critical to mitigating its impact on confined and rangeland livestock. A database was developed representing genes expressed at the immature and adult life stages of the stable fly, comprising data obtained from pyrosequencing both immature and adult stages and from small-scale sequencing of an antennal/maxillary palp-expressed sequence tag library. The full-length sequence and expression of 21 transcripts that may have a role in chemosensation is presented, including 13 odorant-binding proteins, 6 chemosensory proteins, and 2 odorant receptors. Transcripts with potential roles in sex determination and reproductive behaviors are identified, including evidence for the sex-specific expression of stable fly doublesex- and transformer-like transcripts. The current database will be a valuable tool for target identification and for comparative studies with other Diptera.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of expressed sequence tags from a significant livestock pest, the stable fly (Stomoxys calcitrans), identifies transcripts with a putative role in chemosensation and sex determination

Olafson

Lohmeyer

Dowd

2010

Arch Insect Biochem Physiol

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“…stephensi , 14 followed by identification in the sialome of Aedes albopictus , 11 and more recently, in the sialome of the stable fly, Stomoxys calcitrans where it was found abundantly expressed. 41 Sim-692 is 29% identical and 48% similar in its sequence to the An. Protein families exclusive to black flies.…”

Section: Ubiquitous Protein Families Of Characterized Function(s)mentioning

confidence: 95%

“…36,37 Endonucleases, together with hyaluronidases may also be present, possibly decreasing the skin viscosity and helping the diffusion of salivary pharmaceuticals and formation of the feeding cavity in poolfeeding insects such as sand flies and black flies. 13,[38][39][40][41][42][43] Apyrase is also commonly found; it hydrolyzes host ATP and ADP to adenosine monophosphate (AMP), thus depleting these agonists of neutrophil and platelet aggregation. 6,7,44 The sialotranscriptome of S. nigrimanum reveals the presence of at least two glycosidases, possibly polymorphic as demonstrated by assembly of related coding sequences from multiple ESTs (Supplemental Table S1).…”

Section: Ubiquitous Protein Families Of Characterized Function(s)mentioning

confidence: 99%

An Insight into the Sialotranscriptome of Simulium nigrimanum, a Black Fly Associated with Fogo Selvagem in South America

Ribeiro

Valenzuela

Pham

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Abstract. Pemphigus foliaceus is a life threatening skin disease that is associated with autoimmunity to desmoglein, a skin protein involved in the adhesion of keratinocytes. This disease is endemic in certain areas of South America, suggesting the mediation of environmental factors triggering autoimmunity. Among the possible environmental factors, exposure to bites of black flies, in particular Simulium nigrimanum has been suggested. In this work, we describe the sialotranscriptome of adult female S. nigrimanum flies. It reveals the complexity of the salivary potion of this insect, comprised by over 70 distinct genes within over 30 protein families, including several novel families, even when compared with the previously described sialotranscriptome of the autogenous black fly, S. vittatum. The uncovering of this sialotranscriptome provides a platform for testing pemphigus patient sera against recombinant salivary proteins from S. nigrimanum and for the discovery of novel pharmacologically active compounds.*Address correspondence to José M. C. Ribeiro, 12735 Twinbrook Parkway room 2E32D, Rocvkville, MD 20852. E-mail: jribeiro@niaid .nih.gov This paper should be a guide to browsing the supplemental files S1 and S2, found at: http://exon.niaid.nih.gov/transcriptome/S_nigrima num/S1/Sn-S1-web.xls ; http://exon.niaid.nih.gov/transcriptome/S_nig rimanum/S2/Sn-S2-web.xls . 1061SIMULIUM SIALOTRANSCRIPTOME strand. This sequence is then used in subsequent PCR reactions and restriction digests.First-strand synthesis was carried out using PowerScript reverse transcriptase at 42°C for 1 hour in the presence of the SMART IV and CDS III (3′) primers. Second-strand synthesis was performed using a long distance (LD) PCR-based protocol, using Advantage Taq polymerase (Clontech) mix in the presence of the 5′ PCR primer and the CDS III (3′) primer. The cDNA synthesis procedure resulted in creation of SfiI A and B restriction enzyme sites at the ends of the PCR products that are used for cloning into the phage vector (lambda TriplEx2 vector, Clontech). The PCR conditions were as follows: 95°C for 1 min; 24 cycles of 95°C for 10 sec, 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on a 1.1% agarose gel to check quality and range of cDNA synthesized. Double-stranded cDNA was immediately treated with proteinase K (0.8 μg/mL) at 45°C for 20 min, and the enzyme was removed by ultrafiltration though a Microcon (Amicon Inc., Beverly, CA) YM-100 centrifugal filter device. The cleaned, double-stranded cDNA was then digested with SfiI at 50°C for 2 hours, followed by size fractionation on a ChromaSpin-400 column (Clontech) into small (S), medium (M), and large (L) transcripts based on their electrophoresis profile on a 1.1% agarose gel. Selected fractions were pooled and concentrated using a Microcon YM-100.The concentrated cDNA mixture was ligated into the λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using the GigaPack III Plus packaging extract (Stratagene, La Jolla, CA) according ...

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“…Kazal-type peptidase inhibitors in the salivary glands of hematophagous insects are highly specialized regulators of thrombin activity with K i about 10 À13 M (Friedrich et al, 1993) and effectively suppress the hemostatic and inflammatory reactions in the host. Analyses of the salivary glands transcriptome (sialome) of blood-sucking Diptera revealed a vast repertoire of peptides, some of them with Kazal domains, participating in the anti-clotting reactions (Calvo et al, 2009;Wang et al, 2009). In some hematophagous insects, Kazal inhibitors produced by the gut epithelium prevent coagulation of ingested blood.…”

Section: Namementioning

confidence: 99%

Peptidase inhibitors from the salivary glands of the cockroach Nauphoeta cinerea

Taranushenko

Vinokurov

Kłudkiewicz

et al. 2009

Insect Biochemistry and Molecular Biology

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An insight into the transcriptome and proteome of the salivary gland of the stable fly, Stomoxys calcitrans

Cited by 34 publications

References 63 publications

Analysis of expressed sequence tags from a significant livestock pest, the stable fly (Stomoxys calcitrans), identifies transcripts with a putative role in chemosensation and sex determination

Analysis of expressed sequence tags from a significant livestock pest, the stable fly (Stomoxys calcitrans), identifies transcripts with a putative role in chemosensation and sex determination

An Insight into the Sialotranscriptome of Simulium nigrimanum, a Black Fly Associated with Fogo Selvagem in South America

Peptidase inhibitors from the salivary glands of the cockroach Nauphoeta cinerea

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