An Insight into Total Soluble Proteins across Rice (Oryza sativa L.) Germplasm Accessions

Aiyswaraya, S.; Saraswathi, R.; Ramchander, Sanjay; Vinoth, R.; Uma, D; Sudhakar, D.; Robin, S.

doi:10.20546/ijcmas.2017.612.261

Cited by 4 publications

(3 citation statements)

References 7 publications

(9 reference statements)

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“…The above steps were performed for each entry separately until a clear extract was obtained. The extract was stored in deep freezer until further analysis" [9].…”

Section: Sample Preparationmentioning

confidence: 99%

“…Determination of protein concentration by ultraviolet absorption depends on the presence of aromatic amino acids in the proteins. Although different proteins will have different amino acid compositions and thus different molar absorptivities, this method can be very accurate when comparing different solutions of the same protein" [10]. "Extracted samples of 0.2 ml were taken into test tube and the volume was made up to 1 ml with distilled water.…”

Section: Protocolmentioning

confidence: 99%

“…The contents were mixed well and the absorbance was measured at 650 nm after 15 minutes in a spectrophotometer. From the standard graph, the amount of protein in the given unknown solution was calculated" [9].…”

Section: Protocolmentioning

confidence: 99%

See 2 more Smart Citations

Variation in Crude Protein Content among Recombinant Inbred Lines of Rice (Oryza sativa L.)

Lakshmeesha R,

Mahesh, H.B.,

Pattanashetti

et al. 2024

EJNFS

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Rice, Oryza sativa L., is the world's most important staple crop, feeding more than half of the world's population, protein plays important role in human nutrition. Protein is the second most abundant storage matter in rice grain. Protein, crucial for growth, antibody production, and immunity, ranks as the second most abundant storage component in rice grains. Various factors, including grain protein content, amino acid composition, and fat content, influence the nutritional quality of rice, with grain protein content being particularly significant. This study conducted screening and evaluation of crude protein content across 200 recombinant inbred lines of rice, derived from an inter-specific cross between BPT5204 and HPR14 parents. Results indicated a range of total crude protein content from 14.99 (mg/g) to 28.11 (mg/g) among samples. Grouping analysis based on available crude protein content categorized the lines into three major groups: approximately 3% (7 lines) exhibited significantly higher protein content (>25mg/g), 59% (119 lines) displayed moderate content (20 mg/g to 25 mg/g), and 38% (76 lines) showed low protein content (<20 mg/g). The identification of high protein content genotypes in this study holds potential for mapping key genomic regions associated with protein content in rice.

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“…The above steps were performed for each entry separately until a clear extract was obtained. The extract was stored in deep freezer until further analysis" [9].…”

Section: Sample Preparationmentioning

confidence: 99%

Section: Protocolmentioning

confidence: 99%

See 1 more Smart Citation

Variation in Crude Protein Content among Recombinant Inbred Lines of Rice (Oryza sativa L.)

Lakshmeesha R,

Mahesh, H.B.,

Pattanashetti

et al. 2024

EJNFS

View full text Add to dashboard Cite

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Genetic improvement of rice grain quality

Samantara¹,

Reyes²,

Mondal³

et al. 2023

QTL Mapping in Crop Improvement

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Morpho-Physiological Phenotyping of Fifteen Farmers Rice Varieties and Assessment of Grain Quality

Sarker

Roy

2023

Curr Agri Res Jour

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The present study high-lighted on the yet-explored extensive analyses of eating and cooking quality parameters in fifteen indigenous rice varieties. As rice is a vital staple crop globally that can feed one-third world’s population solely, it is an imperious task to develop research for enhancement of its nutritional as well as eating and cooking quality (ECQ). The exploitation of identified nutrient-enriched rice germplasm for breeding application is the easiest way to get better quality rice. Satabdi IET-4786 (SAT) appeared to be very demanding by virtue of having sufficient level of amylose which is considered as key determinant of grain quality. The importance of Sabita (SAB), with regards to higher content of amylose and maltose with higher α-amylase activity and of Dudheshwar (DUD) with respect to protein and total free amino acid content in rice grain was also significant. Here we analyzed fifteen different local rice germplasms including aromatic and some high yielding cultivars to identify some qualitative as well as some quantitative traits related to nutrition and consumers’ acceptance. The knowledge gained from this study will largely provide a path direction for the making a proper decision of considering germplasms for large-scale cultivation and dietary consumption.

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An Insight into Total Soluble Proteins across Rice (Oryza sativa L.) Germplasm Accessions

Cited by 4 publications

References 7 publications

Variation in Crude Protein Content among Recombinant Inbred Lines of Rice (Oryza sativa L.)

Variation in Crude Protein Content among Recombinant Inbred Lines of Rice (Oryza sativa L.)

Genetic improvement of rice grain quality

Morpho-Physiological Phenotyping of Fifteen Farmers Rice Varieties and Assessment of Grain Quality

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