An intact F 1 ATPase ⋅-subunit gene and a pseudogene with differing genomic organization are detected in both male-fertile and CMS petunia mitochondria

Yesodi, Vered; Hauschner, Hagit; Tabib, Yona; Firon, Nurit

doi:10.1007/s002940050287

Cited by 10 publications

(10 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some transcripts are edited and others are unedited at this stop codon site . This genomic DNA region has also been cloned from a line 3699 phage library by Yesodi et al (1997). The sequence determined by both laboratories is identical, but Yesodi et al (1997) did not report the presence of this ORF.…”

Section: Locus In Fertile Linesmentioning

confidence: 99%

“…This genomic DNA region has also been cloned from a line 3699 phage library by Yesodi et al (1997). The sequence determined by both laboratories is identical, but Yesodi et al (1997) did not report the presence of this ORF. The nad3 and rps12 coding sequences found in lines 3704, 3688, and 3699 are identical.…”

Section: Locus In Fertile Linesmentioning

confidence: 99%

“…The genomic DNA would predict the presence of an nad3 gene carrying a 185-codon N-terminal extension that contains 133 codons that are nearly identical to those of orf143 in line 3704. However, an RNA editing event Hanson et al (1989), Rasmussen and Hanson (1989), Wilson (1995), and Young and Hanson (1987), except atpA data from Yesodi et al (1997). creates a stop codon, shortening the ORF to 161 codons.…”

Section: Locus In Fertile Linesmentioning

confidence: 99%

“…According to hybridization analysis (data not shown), a region of similarity to the end of the orf143 coding region is located near the 3688 atpA pseudogene ( Figure 4B). A region surrounding this 3688 atpA pseudogene was sequenced by Yesodi et al (1997), but a pairwise comparison of orf143 and the sequenced 3688 atpA pseudogene region reveals no significant similarity. Further sequencing is necessary to verify the presence of additional orf143-homologous sequences near the 3688 atpA pseudogene.…”

Section: Locus In Fertile Linesmentioning

confidence: 99%

“…This genomic sequence includes a portion of an atpA pseudogene and an open reading frame (orf143) that could code for a 16.9 kDa protein ( Figure 2). Yesodi et al (1997) determined the sequence of the entire atpA pseudogene and they sequenced the normal atpA gene for comparison.…”

Section: Locus In Fertile Linesmentioning

confidence: 99%

See 4 more Smart Citations

Mitochondrial gene organization and expression in petunia male fertile and sterile plants

Hanson

Wilson²,

Bentolila³

et al. 1999

Journal of Heredity

View full text Add to dashboard Cite

In cytoplasmic male-sterile Petunia lines, NADH dehydrogenase subunit 3 (nad3) and ribosomal protein S12 (rps12) are cotranscribed with the chimeric gene pcf and located in the region of the mitochondrial genome associated with cytoplasmic male sterility (CMS) in Petunia. In fertile Petunia line 3704, the genes for nad3 and rps12 are cotranscribed with an unidentified open reading frame (orf143). In the homologous region of fertile line 3699, there is an ORF that lacks a genomic DNAencoded stop codon; instead an RNA editing event creates a stop codon, resulting in an ORF of 161 codons. While expressed sequences homologous to this open reading frame can be detected in sterile lines, a contiguous orf143/orf161 gene does not exist in the CMS-encoding mitochondrial genome. Transcription at the CMS-associated pcf locus and the fertile orf143/nad3/rps12 locus is complex, with multiple 5 and 3 termini. The presence of the nuclear fertility restorer gene affects the abundance of a transcript class with 5 termini Ϫ121 nucleotides before the pcf start codon, and greatly reduces the abundance of a pcf gene product with apparent molecular mass of 25 kDa which is present in both vegetative and reproductive tissues of CMS plants. In addition to the 25 kDa protein product, small amounts of precursor and processed pcf products with higher molecular mass have been detected; their possible role in the CMS phenotype is unknown. Current hypotheses for the mechanism of action of CMS-associated and fertility restorer genes are discussed.A region of the Petunia mitochondrial genome was originally correlated with cytoplasmic male sterility (CMS) by observation of its presence in a collection of sterile somatic hybrids and its absence in all fertile somatic hybrids that were examined . The somatic hybrids contained recombinant mitochondrial genomes created following protoplast fusion between CMS and normal fertile parents ( Boeshore et al. 1983;Hanson 1984). Sequencing of the CMS-associated region revealed the presence of an abnormal fused open reading frame called pcf (for petunia CMS-associated fused gene) and apparently normal copies of the nad3 ( NADH dehydrogenase subunit 3) and rps12 (ribosomal protein small subunit protein 12) genes Rasmussen and Hanson 1989;Young and Hanson 1987). A second correlation of the pcf locus with CMS was the finding that the presence of a nuclear-encoded fertility restorer gene Rf alters the abundance of transcripts at the pcf locus Young and Hanson 1987). A third correlation of the pcf locus and CMS was made when a PCF protein product of an apparent molecular mass of 25 kDa was found to decrease greatly in abundance in lines carrying Rf .Despite much knowledge about the gene structure and expression of CMS-associated loci in Petunia (reviewed in Conley and Hanson 1995;Hanson 1991;Hanson and Conde 1985;Hanson et al. , 1988Hanson et al. , 1989Hanson et al. , 1991Hanson et al. , 1995 and other plants such as Brassica, maize, sorghum, bean, and rice, the molecular mechanism of the sterility and fertility ...

show abstract

Section: Locus In Fertile Linesmentioning

confidence: 99%

Section: Locus In Fertile Linesmentioning

confidence: 99%