An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale

Brenelli, Lívia Beatriz; Figueiredo, Fernanda Lopes de; Damásio, André; Franco, Telma Teixeira; Rabelo, Sarita Cândida

doi:10.1016/j.biortech.2020.123637

Cited by 59 publications

(26 citation statements)

References 43 publications

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“…The substrates were vinasse and filter cake from Iracema sugarcane mill (São Martinho group, Iracemápolis, São Paulo state, Brazil) and the liquor from the straw pretreatment process, performed at the National Biorenovables Laboratory (LNBR) from the Brazilian Center for Research in Energy and Materials (CNPEM). Deacetylation pre-treatment was applied to sugarcane straw on a bench-scale as described in Brenelli et al (2020). The anaerobic consortium of the mesophilic reactor (BIOPAC®ICX - Paques) from the aforementioned Iracema mill was used as inoculum.…”

Section: Methodsmentioning

confidence: 99%

Operational and biochemical aspects of co-digestion (co-AD) from sugarcane vinasse, filter cake and deacetylation liquor

Volpi

Júnior

Franco

et al. 2021

Preprint

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This work performed co-AD from the vinasse and filter cake (from 1G ethanol production) and deacetylation liquor (from the pre-treatment of sugarcane straw for 2G ethanol production) in a semi-Continuous Stirred Tank Reactor (s-CSTR) aiming to provide optimum operational parameters for continuous CH4 production. Using filter cake as co-substrate may allow the reactor to operate throughout the year, as it is available in the sugarcane off-season, unlike vinasse. A comparison was made from the microbial community of the seed sludge and the reactor sludge when CH4 production stabilized. Lactate, butyrate and propionate fermentation routes were denoted at the start-up of the s-CSTR, characterizing the acidogenic phase: the Oxidation-Reduction Potential (ORP) values ranged from -800 to -100 mV. Once the methanogenesis was initiated, alkalizing addition was no longer needed as its demand by the microrganisms was supplied by the alkali-characteriscs of the deacetylation liquor. The gradual increase of the applied Organic Load Rates (OLR) allowed stabilization of the methanogenesis from 3.20 gVS L-1 day-1: the highest CH4 yield (230 NmLCH4 gSV-1) and average organic matter removal efficiency (83% + 13) was achieved at ORL of 4.16 gVS L-1day-1. The microbial community changed along the reactor operation, presenting different metabolic routes mainly due to the used lignocellulosic substrates. Bacteria from the syntrophic acetate oxidation (SAO) process coupled to hydrogenotrophic methanogenesis were predominant (~ 2% Methanoculleus) during the CH4 production stability. The overall results are useful as preliminary drivers in terms of visualizing the co-AD process in a sugarcane biorefinery integrated to scale.

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Section: Methodsmentioning

confidence: 99%

Operational and biochemical aspects of co-digestion (co-AD) from sugarcane vinasse, filter cake and deacetylation liquor

Volpi

Júnior

Franco

et al. 2021

Preprint

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“…The process was carried out in a at 60 °C with 80 mg NaOH·g biomass -1 and 10% (w/w) of final solid loading, immersed in a glycerin bath. Conditions were set based on a previous study to optimize XOS production [8]. The liquid fraction is mainly composed by acetate and phenolic compounds from lignin and extractives and was recovered through straining using a muslin cloth.…”

Section: Methodsmentioning

confidence: 99%

“…For the filter cake, the TS value was higher than normally reported (literature:0.21-0.28 g mL -1 ) [2], while VS content was much lower (literature:0.70-0.74 g mL -1 ) [9]. Such variations reflect the variability of ethanol production processes and the agricultural procedures affecting biomass characteristics, as well as the sazonality of sugarcane, already stated The deacetylation liquor presented a strong alkali characteristic since it came from a mild alkaline pretreatment of sugarcane straw to remove acetyl groups and promote lignin solubilization [8]. Alkaline pretreatment is typically used in lignocellulosic materials such as wheat straw and sugarcane bagasse, thus decreasing its recalcitrance [3].…”

Section: ‫ܥ‬mentioning

confidence: 96%

“…Pretreatment was carried out at 60°C, 80 mg NaOH g 1 biomass -1 and 10% (w/w) of final solid loading, in a 316 L stainless steel reactor of 0.5 1 L capacity, immersed in a glycerin bath. The pretretreatment conditions were obtained 1 based on a previous study to optimize XOS production, whose liquor is considered a 1 residue of this process [8]. The liquid fraction mainly composed by acetate and phenolic 1 compounds from lignin and extractives, referred to as deacetylation liquor, was 1 recovered by straining it through a muslin cloth and stored at 4ºC for further use.…”

Section: Substrates and Inoculummentioning

confidence: 99%

“…Sugarcane straw has a similar chemical composition to bagasse in terms of the major components of biomass: cellulose (30-40% w/w), hemicelluloses (20-30% w/w), and lignin (15-30% w/w) [11]. This biomass can be converted into value-added products as biofuels, after pretreatment methods and multienzyme complexes to liberate sugars Among the diversity of methods that have been researching aiming at technological process improvements, Brenelli et al [12] recently reported a promising alkaline pre-treatment of sugarcane straw by deacetylation, in which acetic acid is removed as it is an inhibitor for microorganisms in fermentation processes, and thus, xylo-oligosaccharide (XOS) are recovered for being fermented to ethanol. Filter cake, another organic solid byproduct, is generated from the filtration in rotary filters after cane juice clarification processes, presenting concentrations of 140-169 g kg -1 of lignin, 171-184.6 g kg -1 of cellulose and 153-170 g kg -1 of hemicellulose [3, 13].…”

Section: Introductionmentioning

confidence: 99%

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Use of lignocellulosic residue from second-generation ethanol production to enhance methane production through co-digestion

Volpi

Brenelli

Mockaitis

et al. 2021

Preprint

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This work evaluated the methane (CH4) production potential from residues of integrated 1st (vinasse and filter cake) and 2nd (deacetylation pretreatment liquor from straw) generation (1G2G) sugarcane biorefinery. The small-scale study provided fundamentals for basing the optimization of co-digestion by assessing the best co-substrates synergistic conditions. Biochemical Methane Potential (BMP) tests showed co-digestion enhanced CH4 yield of isolated substrates, reaching 618 NmLCH4 gVS-1, and all of the experiments were well describe by modified Gompertz model. Vinasse and deacetylation liquor as the only co-substrates increased the BMP by 36.75%, indicating that the association of these two residues provided positive effects for co-digestion by nutritionaly benefeting the methanogenic activity. The filter cake had the lowest BMP (260 NmLCH4 gVS-1) and digestibility (<40%), being the stirring required to improve the mass transfer of biochemical reactions. The alkaline characteristic of the liquor (pH-12) prevented alkalinizers from being added to the co-digestion, which could be a relevant economic advantage for the implementation of the process in an industrial scale. The co-digestion system has proven to efficiently maximize waste management in the 1G2G sugarcane biorefineries and potentially enhance their energy generation (by at least in 18%), providing experimental elements for placing the biogas production as the hub of the bioeconomy in the agroindustrial sector.

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Production of cello‐oligosaccharides through the biorefinery concept: A technical‐economic and life‐cycle assessment

Barbosa

Nogueira

Kendrick

et al. 2021

Biofuels Bioprod Bioref

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Producers look for co-products to increase the value chain and production flexibility. With a high market aggregate value, the production of cello-oligosaccharides (COS) as functional oligosaccharides from cellulosic substrates has been investigated. As COS/cellopentaose production process are rarely described in the literature, this study estimated the cellopentaose production cost and performed a lifecycle assessment of the cellopentaose production process previously developed using sugarcane straw as a substrate. The results demonstrated that, through the six scenarios studied, it was possible to obtain a cellopentaose unit production cost varying between USD 0.40 and 1.15/mg, and that optimization in the upstream sector can reduce the total plant direct cost and the overall life-cycle impact. The results also

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An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale

Cited by 59 publications

References 43 publications

Operational and biochemical aspects of co-digestion (co-AD) from sugarcane vinasse, filter cake and deacetylation liquor

Operational and biochemical aspects of co-digestion (co-AD) from sugarcane vinasse, filter cake and deacetylation liquor

Use of lignocellulosic residue from second-generation ethanol production to enhance methane production through co-digestion

Production of cello‐oligosaccharides through the biorefinery concept: A technical‐economic and life‐cycle assessment

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