An integrated approach to the study of Hypericum occurring in Sicily

Giovino, Antonio; Carrubba, Alessandra; Lazzara, Silvia; Napoli, Edoardo; Domina, Gianniantonio

doi:10.3906/bot-1912-34

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…The majority of the components in the extracts from H. perforatum flowers resulted phloroglucinols (hyperforins) and flavonols (myricetin derivates, rutin, myricitrin, hyperoside, isoquercitrin, quercitrin, and quercetin), adding up from 68% to 84% of total identified phenols. This feature is typical of H. perforatum, and allows a rather precise separation of this species from many others, even if taxonomically close [25,30].…”

Section: Phytochemical Compositionmentioning

confidence: 76%

Cultivating for the Industry: Cropping Experiences with Hypericum perforatum L. in a Mediterranean Environment

2021

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Hypericum perforatum is an intensively studied medicinal plant, and much experimental activity has been addressed to evaluate its bio-agronomical and phytochemical features as far. In most cases, plant material used for experimental purposes is obtained from wild populations or, alternatively, from individuals grown in vases and/or pots. When Hypericum is addressed to industrial purposes, the most convenient option for achieving satisfactory amounts of plant biomass is field cultivation. Pot cultivation and open field condition, however, are likely to induce different responses on plant’s metabolism, and the obtained yield and composition are not necessarily the same. To compare these management techniques, a 4-year cultivation trial (2013–2016) was performed, using three Hypericum biotypes obtained from different areas in Italy: PFR-TN, from Trento province, Trentino; PFR-SI, from Siena, Tuscany; PFR-AG, from Agrigento province, Sicily. Both managements gave scarce biomass and flower yields at the first year, whereas higher yields were measured at the second year (in open field), and at the third year (in pots). Plant ageing induced significant differences in phytochemical composition, and the total amount of phenolic substances was much higher in 2015 than in 2014. A different performance of genotypes was observed; the local genotype was generally more suitable for field cultivation, whereas the two non-native biotypes performed better in pots. Phytochemical profile of in-pots plants was not always reflecting the actual situation of open field. Consequently, when cultivation is intended for industrial purposes, accurate quality checks of the harvested material are advised.

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Section: Phytochemical Compositionmentioning

confidence: 76%

Cultivating for the Industry: Cropping Experiences with Hypericum perforatum L. in a Mediterranean Environment

2021

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“…This is due to the new and different species delimitation adopted in the updated checklist of Italy (Bartolucci et al 2018;Galasso et al 2018) and to the increased knowledge of plant taxonomy in recent years (e.g. Astuti et al 2017;Domina et al 2017;Giovino et al 2020).…”

Section: An Updated Cwr/whp Checklist For Italymentioning

confidence: 99%

A new list and prioritization of wild plants of socioeconomic interest in Italy: toward a conservation strategy

Ciancaleoni

Raggi

Barone

et al. 2021

Agroecology and Sustainable Food Systems

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Wild harvested plants and crop wild relatives, part of the segment of natural diversity that is collectively known as "Plant Genetic Resources", have great socio-economic importance for humans because they are used either directly or in crop breeding. In order to lay down a solid base for constructing conservation strategies for Italy, an updated annotated list of CWR and WHP was produced for the country including information on known uses. Taxa included in the list were then prioritised using a pragmatic approach based on their value, native status and need of protection or monitoring.

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“…Reliable molecular identification depends on the resolution of molecular markers (or DNA barcodes) and the species coverage of the reference library. Considerable efforts have been made to find the ideal DNA barcodes for plants (CBOL Plant Working Group, 2009;Dong et al, 2014Dong et al, , 2015Kress & Erickson, 2007;Li et al, 2011) as well as to develop new technical improvements (Giovino et al, 2020a;Hollingsworth et al, 2011;Xu et al, 2015;Yu et al, 2011). Unlike animals which COI (mitochondrial cytochrome oxidase I) is a nearly sole DNA barcode, plant DNA barcoding is much more complicated and no ideal DNA barcodes for plants have yet been discovered, or perhaps they do not exist at all (Giovino et al, 2020b).…”

Section: Introductionmentioning

confidence: 99%

Method for quick DNA barcode reference library construction

Liu

Sun

et al. 2021

Ecology and Evolution

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DNA barcoding has become one of the most important techniques in plant species identification. Successful application of this technology is dependent on the availability of reference database of high species coverage. Unfortunately, there are experimental and data processing challenges to construct such a library within a short time. Here, we present our solutions to these challenges. We sequenced six conventional DNA barcode fragments (ITS1, ITS2, matK1, matK2, rbcL1, and rbcL2) of 380 flowering plants on next‐generation sequencing (NGS) platforms (Illumina Hiseq 2500 and Ion Torrent S5) and the Sanger sequencing platform. After comparing the sequencing depths, read lengths, base qualities, and base accuracies, we conclude that Illumina Hiseq2500 PE250 run is suitable for conventional DNA barcoding. We developed a new “Cotu” method to create consensus sequences from NGS reads for longer output sequences and more reliable bases than the other three methods. Step‐by‐step instructions to our method are provided. By using high‐throughput machines (PCR and NGS), labeling PCR, and the Cotu method, it is possible to significantly reduce the cost and labor investments for DNA barcoding. A regional or even global DNA barcoding reference library with high species coverage is likely to be constructed in a few years.

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An integrated approach to the study of Hypericum occurring in Sicily

Cited by 4 publications

References 24 publications

Cultivating for the Industry: Cropping Experiences with Hypericum perforatum L. in a Mediterranean Environment

Cultivating for the Industry: Cropping Experiences with Hypericum perforatum L. in a Mediterranean Environment

A new list and prioritization of wild plants of socioeconomic interest in Italy: toward a conservation strategy

Method for quick DNA barcode reference library construction

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