An integrated approach to uncover quality markers of Traditional Chinese medicine underlying chemical profiling, network target selection and metabolomics approach: Guan-Xin-Jing capsule as a model

Zhang, Guanhua; Wang, Qi; Liu, Xin; Wang, Xu; Xie, Wenyu; Liu, Kun; Wujun, Jiang; Mu, Chenglin; Qi, Huanhuan; Wang, Qiao

doi:10.1016/j.jpba.2020.113413

Cited by 7 publications

(2 citation statements)

References 23 publications

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“…Currently, underlying component content and profiling, many useful methods have been developed to identify proteins and peptides at the transcriptomic, peptidomic, or genomic level to analyze the active substances, reveal the quality markers in medicinal materials, and perform an overall quality evaluation through bioinformatics and validation [33][34][35][36]. Here, transcriptomics and tandem mass tag (TMT) proteomics were used to analyze differences and identify quality markers in protein components of toad venom from different regions.…”

Section: Introductionmentioning

confidence: 99%

Identification of Protein Quality Markers in Toad Venom from Bufo gargarizans

et al. 2023

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Toad venom is a traditional Chinese medicine with high medicinal value. The existing quality evaluation standards of toad venom have obvious limitations because of the lack of research on proteins. Thus, it is necessary to screen suitable quality markers and establish appropriate quality evaluation methods for toad venom proteins to guarantee their safety and efficacy in clinical applications. SDS-PAGE, HPLC, and cytotoxicity assays were used to analyze differences in protein components of toad venom from different areas. Functional proteins were screened as potential quality markers by proteomic and bioinformatic analyses. The protein components and small molecular components of toad venom were not correlated in content. Additionally, the protein component had strong cytotoxicity. Proteomics analysis showed that 13 antimicrobial proteins, four anti-inflammatory and analgesic proteins, and 20 antitumor proteins were differentially expressed extracellular proteins. A candidate list of functional proteins was coded as potential quality markers. Moreover, Lysozyme C-1, which has antimicrobial activity, and Neuropeptide B (NPB), which has anti-inflammatory and analgesic activity, were identified as potential quality markers for toad venom proteins. Quality markers can be used as the basis of quality studies of toad venom proteins and help to construct and improve safe, scientific, and comprehensive quality evaluation methods.

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Section: Introductionmentioning

confidence: 99%

Identification of Protein Quality Markers in Toad Venom from Bufo gargarizans

et al. 2023

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“…The strategy of identification or tentatively characterization of chemical components by conventional identification method from the literature [12, 13] was as follows: Firstly, an in‐house database about the chemical components from five herbs was established by searching the literature and database including PubChem (https://www.ncbi.nlm.nih.gov/) and ChemsSpider (http://www.chemspider.com/). Next, compounds in the database were inputted into the MasslynxV4.1 software, and the peaks with the same formula were screened out.…”

Section: Methodsmentioning

confidence: 99%

Dissection of the potential immunomodulatory activity materials and mechanism of Wu Shen Wan (a classical traditional Chinese medicine prescription) based on in vivo substances profiling and network pharmacology

Zhang,

Han,

et al. 2024

Separation Science Plus

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In the present study, a comprehensive strategy based on substances profiling in vivo and network pharmacology method was applied to screen out the potential immunomodulatory activity substances and mechanism of Wu Shen Wan. As a result, a total of 121 constituents were identified or tentatively characterized, including 26 flavonoids, 16 alkaloids, 10 tanshinones, four diterpenes, seven iridoid glycosides, eight coumarin, 18 phenolic acids, 29 ginsenosides, and three others. Moreover, 57 prototypes were clearly screened out in rat plasma and urine after ingestion of Wu Shen Wan. The Wu Shen Wan exhibited immunomodulatory effects via significantly increasing the phagocytic activity of macrophages and inducing the release of nitric oxide, tumor necrosis factor‐α, and interleukin 6 in RAW264.7 cells. In addition, the network pharmacology results showed that the top 20 Kyoto Encyclopedia of Genes and Genomes clusters were enriched, such as mitogen‐activated protein kinases pathway. This is the first systematic study on the chemical constituents of Wu Shen Wan. The analytical method features a quick and comprehensive dissection of the chemical composition and provides a basis for exploring its immunomodulatory effects.

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