2018
DOI: 10.1016/j.scitotenv.2018.04.223
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An integrated cell absorption process and quantitative PCR assay for the detection of the infectious virus in water

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Cited by 12 publications
(10 citation statements)
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“…To find a more feasible strategy to detect infectious viruses only, this review focuses on methods involving pretreatment of the virus before qPCR, such as ICC-qPCR, that make it applicable to virus inactivation validation studies (Balkin & Margolin, 2010;Blackmer, Reynolds, Gerba, & Pepper, 2000;Chapron et al, 2000;Gerrity, Ryu, Crittenden, & Abbaszadegan, 2008;Greening, Hewitt, & Lewis, 2010;Grimm, Cashdollar, Williams, & Fout, 2004;Guo, Wang, Zhao, Li, & Zhong, 2018;Jiang et al, 2010;Ko, Cromeans, & Sobsey, 2003;Ko et al, 2005;S. H. Lee, Lee, Lee, Cho, & Kim, 2005;D.…”
Section: Icc-qpcr Methodsmentioning
confidence: 99%
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“…To find a more feasible strategy to detect infectious viruses only, this review focuses on methods involving pretreatment of the virus before qPCR, such as ICC-qPCR, that make it applicable to virus inactivation validation studies (Balkin & Margolin, 2010;Blackmer, Reynolds, Gerba, & Pepper, 2000;Chapron et al, 2000;Gerrity, Ryu, Crittenden, & Abbaszadegan, 2008;Greening, Hewitt, & Lewis, 2010;Grimm, Cashdollar, Williams, & Fout, 2004;Guo, Wang, Zhao, Li, & Zhong, 2018;Jiang et al, 2010;Ko, Cromeans, & Sobsey, 2003;Ko et al, 2005;S. H. Lee, Lee, Lee, Cho, & Kim, 2005;D.…”
Section: Icc-qpcr Methodsmentioning
confidence: 99%
“…Subsequently, the cells are incubated for an optimized period to amplify the intracellular viruses, decrease the lower limit of quantitation (LLOQ) and improve the sensitivity of detection. Finally, the nucleic acids in the cells and viruses are extracted and then used for qPCR with virus-specific primers to quantify the infectious virus after virus inactivation (Gerrity et al, 2008;Guo et al, 2018;F. Li et al, 2009;Ryu et al, 2015).…”
Section: Icc-qpcr Methodsmentioning
confidence: 99%
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