“…To find a more feasible strategy to detect infectious viruses only, this review focuses on methods involving pretreatment of the virus before qPCR, such as ICC-qPCR, that make it applicable to virus inactivation validation studies (Balkin & Margolin, 2010;Blackmer, Reynolds, Gerba, & Pepper, 2000;Chapron et al, 2000;Gerrity, Ryu, Crittenden, & Abbaszadegan, 2008;Greening, Hewitt, & Lewis, 2010;Grimm, Cashdollar, Williams, & Fout, 2004;Guo, Wang, Zhao, Li, & Zhong, 2018;Jiang et al, 2010;Ko, Cromeans, & Sobsey, 2003;Ko et al, 2005;S. H. Lee, Lee, Lee, Cho, & Kim, 2005;D.…”