An integrated droplet-digital microfluidic system for on-demand droplet creation, mixing, incubation, and sorting

Ahmadi, Fatemeh; Samlali, Kenza; Vo, Philippe Q. N.; Shih, Steve C. C.

doi:10.1039/c8lc01170b

Cited by 72 publications

(72 citation statements)

References 73 publications

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“…By using the electric field, there is no special requirement for the design of microchannel structure. Consequently, the microfluidic chip is simple and easy to manufacture . Recently, Deng and his colleagues reported an electric‐field‐dependent droplet selector for the manipulation of droplets on a superhydrophobic surface.…”

Section: Microfluidics For the Fabrication And Manipulation Of Dropletsmentioning

confidence: 99%

“…[77] In this work, they used a device which consists of two T-shaped junctions and a Y-junction. These two parts were used to form droplets and to mix the Small 2020, 16,1903940 Creation, mixing, incubation, sorting An integrated droplet-digital microfluidic system [103] Sorting Superhydrophobic interdigitated array chips [104] Floating Movable electrode biased with a high voltage [105] Mixing Open and closed digital microfluidic platforms [106] Coalescence, migrating Polypyrrole whelk-like arrays [107] Generation, transportation, splitting Ionic-surfactant-mediated electro-dewetting digital microfluidics [108] Magnetic Sorting Magnetophoretic sorting system [101] Splitting, dispensing, exchange, trapping, release, demulsification An integrated magnetic repulsion-actuated microfluidic system [111] Mixing, migrating, transport, release, coalescence Ferrofluid-containing liquid-infused porous surfaces [113] Transport, sorting, mixing A magnetic digital microfluidics platform [114] Shape evolution, splitting A device with hydrophobic surface under the magnetic field [115] Transport, splitting, motion Magnetic tubular microactuators [116] Motion, transport, fusion A deformable paramagnetic liquid substrate [117] Acoustic Coalescence A straight channel with a narrow beam surface acoustic wave [119] Splitting, steering A disposable parallel-type SAW-based acoustofluidic device [120] Gravity Transport, collision, fusion, mixing, stopping A gravity-actuated droplet microfluidics device [67] Movement, mixing Polydopamine microfluidic system [121] Trapping, exchange, transfer, fusion A microstructures device possesses microwell arrays and straight microchannel [122] Optical Motion A device with a single focused laser [124] Motion, patterned writing A device with photoelectric cooperative-responsive slippery surface [125] Sorting, dispensing Printed droplet microfluidics [126] Transportation, merging, splitting Light-driven flexible opto-electrowetting devices [127] Mixing, motion, coalescence A pyroelectrotrapping superhydrophobic surface platform [128] Mechanical Mixing, movement, Mechanical-activated digital microfluidics…”

Section: T-junction Devices For the Fabrication Of Dropletsmentioning

confidence: 99%

“…Consequently, the microfluidic chip is simple and easy to manufacture. [102,103] Recently, Deng and his colleagues reported an electric-field-dependent droplet selector for the manipulation of droplets on a superhydrophobic surface. As shown in Figure 5A, the interaction between the drop and the superhydrophobic surfaces can be controlled by the liquid dielectrophoresis force which was generated by the electric-field.…”

Section: Microfluidics For the Manipulation Of Dropletsmentioning

confidence: 99%

See 2 more Smart Citations

Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

Xie

Xiong

et al. 2019

Small

121

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Fabrication of artificial biomimetic materials has attracted abundant attention. As one of the subcategories of biomimetic materials, artificial cells are highly significant for multiple disciplines and their synthesis has been intensively pursued. In order to manufacture robust “alive” artificial cells with high throughput, easy operation, and precise control, flexible microfluidic techniques are widely utilized. Herein, recent advances in microfluidic‐based methods for the synthesis of droplets, vesicles, and artificial cells are summarized. First, the advances of droplet fabrication and manipulation on the T‐junction, flow‐focusing, and coflowing microfluidic devices are discussed. Then, the formation of unicompartmental and multicompartmental vesicles based on microfluidics are summarized. Furthermore, the engineering of droplet‐based and vesicle‐based artificial cells by microfluidics is also reviewed. Moreover, the artificial cells applied for imitating cell behavior and acting as bioreactors for synthetic biology are highlighted. Finally, the current challenges and future trends in microfluidic‐based artificial cells are discussed. This review should be helpful for researchers in the fields of microfluidics, biomaterial fabrication, and synthetic biology.

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Section: Microfluidics For the Fabrication And Manipulation Of Dropletsmentioning

confidence: 99%

Section: T-junction Devices For the Fabrication Of Dropletsmentioning

confidence: 99%

Section: Microfluidics For the Manipulation Of Dropletsmentioning

confidence: 99%

See 1 more Smart Citation

Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

Xie

Xiong

et al. 2019

Small

121

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“…electrode and dielectric) along with a top patterned channel layer in which droplets are manipulated in an oil phase. [45] The device is divided into two sections: 1) an ondemand droplet generator and 2) a single-cell droplet array. As shown in Figure 1B, the ondemand droplet generation consists of co-planar electrodes that will actuate the aqueous flow (using electric potentials) to the orthogonal continuous oil flow that will break the continuous aqueous flow into discretized droplets under the control of the user.…”

Section: 1mentioning

confidence: 99%

“…ground and activated electrodes on the same plate) under microfluidic channels to have individual control of the droplets in channels. [45] Given the increased control of the droplets that hybrid microfluidic technologies have shown [46][47][48][49][50][51] , there is an opportunity to use this technology as a method to control the isolation of mammalian isoclones.…”

Section: Introductionmentioning

confidence: 99%

One cell, one drop, one click: hybrid microfluidic mammalian single-cell isolation

Samlali

Ahmadi

Quach

et al. 2020

Preprint

Self Cite

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The process of generating a stable knockout cell line is a complex process that can take several months to complete. In this work, we introduce a microfluidic method that is capable of isolating single cells, selecting successful edited clones, and expansion of these isoclones. Using a hybrid microfluidics method, droplets in channels can be individually addressed using a co-planar electrode system. In our hybrid microfluidic device, we show that we can trap single cells and subsequently encapsulate them on demand into pL-sized droplets. Furthermore, individual cells inside the droplet can be released from the traps or merged with other droplets by simply applying an electric potential to the electrodes that is actuated through a user interface. We use this high precision control to sort and to recover single isoclones to establish monoclonal cell lines, which is demonstrated with a heterozygous NCI-H1299 lung squamous cell population resulting from loss-of-function eGFP and RAF1 gene knock-out transfections.

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A Digital‐to‐Channel Microfluidic Interface via Inkjet Printing of Silver and UV Curing of Thiol–Enes

Sathyanarayanan

Haapala

Dixon

et al. 2020

Adv Materials Technologies

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mixed, and split in parallel to perform sequential sample manipulation protocols, such as rinsing, preconcentration, reaction, and extraction. Interfacing of DMF with a range of optical, [3,4] electrochemical, [5-7] and mass spectrometric detection [8-11] methods is well established and the fabrication of the devices has already been pushed toward low-cost cleanroom-free techniques. [12,13] As a result, DMF is a robust and cost-effective technology for miniaturization of various biochemical assays including but not limited to immunological, [14] enzymatic, [15] cell-based, [16] PCR, [17] drug, [18] and biopsy [19] screening assays. However, in some instances, the specificity of direct in situ detection may not be sufficient to distinguish multiple sample components from each other, and in these cases, the droplet must be transferred for further analysis into on-chip or off-chip chemical separation systems. To date, much of the prior work has exploited off-chip analysis of DMF-manipulated samples, which is however relatively complicated and prone to sample losses during the droplet transfer from micro-to macro scale. Although a variety of miniaturized electrophoretic and chromatographic separation chips have been reported in general, [20-22] their integration with DMF has not been comprehensively established, likely because of the marked differences in the applicable microfabrication materials and methods. DMF driving electrodes are typically defined on a glass substrate by photolithography. Recently, increasing effort has been put into

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An integrated droplet-digital microfluidic system for on-demand droplet creation, mixing, incubation, and sorting

Abstract: A new microfluidic platform that integrates droplet and digital microfluidics to automate a variety of fluidic operations. The platform was applied to culturing and to selecting yeast mutant cells in ionic liquid.

Cited by 72 publications

References 73 publications

Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

One cell, one drop, one click: hybrid microfluidic mammalian single-cell isolation

A Digital‐to‐Channel Microfluidic Interface via Inkjet Printing of Silver and UV Curing of Thiol–Enes

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