An Integrated Genetic Map for Brassica napus Derived from Double Haploid and Recombinant Inbred Populations

Abstract

A hybrid developed from a cross between two diverse Brassica napus cultivars ("Polo" and "Topas") was used to produce a microspore derived double haploid (DH) population and a single seed descent derived recombinant inbred (RI) population for genetic mapping. Each of the two populations consisting of 190 DH lines and 94 RI lines was characterized for various types (SSR, SRAP, ISSR, SCAR) of polymorphic molecular markers. The DH population was scored for 620 molecular markers while the RI population was scored for 349 molecular markers to construct two independent genetic maps. In both genetic maps, all of the molecular markers were found to cluster in 19 linkage groups (LGs) covered a total genome length of 2244.1 cM and 1649.1 cM for the DH and RI maps, respectively. The data from the two genetic maps was used to construct a consensus integrated genetic map covering a total genome length of 2464.9 cM. Previously published Brassica reference genetic maps were used to assign each of the nineteenLGs to corresponding Brassica napus chromosomes named N01 to N19. To our knowledge, this is the first integrated genetic map based on DH and RI populations developed from the same cross in Brassica napus.

Synteny analysis of genes and distribution of loci controlling oil content and fatty acid profile based on QTL alignment map in Brassica napus

“…It is a very good approach for detecting hot spot genomic regions associated with the traits, but not always accurate for validating exact QTL. These nine populations were produced from hybridization of different varieties of B. napus : DY is a hybrid of European and Korean [ 37 ], SG and TN are hybrids of European and Chinese [ 35 , 67 ], Z5, KN and M201XM202 are Chinese [ 42 , 48 , 68 ], SO is European [ 69 ] and PT is Canadian [ 70 ]. Genesis of variety within a species initiates in natural selection which enables species to settle into specific environmental pressures.…”

Human Embryos Genetically Modified: A Review

“…As per the discussion related to the ethical and religious beliefs, Ronald Cole-Turner who teaches theology and ethics at Pittsburgh Theological Seminary shares his views saying it is needed to have a combined discussion with the different religious believes [114][115][116][117][118][119][120]. He suggests in Christianity and Judaism is surprisingly positive in their assessment of this possibility.…”

Identification of Brassica genotypes and molecular markers for increased seed oil content

“…Polo )cv. Topas cross (Geng et al 2012). The DH line population derived genetic map consisted of 620 polymorphic loci, with mostly SSR markers, and a few ISSR, SRAP and SCAR markers, which were grouped into 19 linkage groups and then assigned to B. napus chromosomes using common SSR markers that were previously published.…”

“…FINDINGS TO SHORT-AND LONG-TERM GREENHOUSE GAS MANAGEMENT The genetic maps developed as part of the Green Crop Network sponsored research at the University of Manitoba (Geng et al 2012) are saturated with the most SSR markers of any available genetic maps in B. napus and will be excellent maps to use to identify QTL for oil content and component fatty acids in canola in the shortterm. They will also provide a solid foundation for the development of genetic maps saturated with even more SSR markers in the long-term.…”