An integrated hypothesis for miR-126 in vascular disease

Yu, Bo; Jiang, Yinghua; Wang, Xiaoying; Wang, Shusheng

doi:10.18103/mra.v8i5.2133

Cited by 19 publications

(11 citation statements)

References 22 publications

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“… 26 These features highlight the functional complexity of this miRNA. As was comprehensively reviewed by Yu et al, 28 miR-126-3p is associated with vascular or related diseases. Endothelial cells exhibit high levels of miR-126, which helps to preserve vascular integrity and promote angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

Alteration of circulating miRNAs during myocardial infarction and association with lipid levels

Ozuynuk-Ertugrul,

Ekici,

Erkan

et al. 2023

Laboratory Medicine

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Background Increasing mortality and morbidity of coronary artery disease (CAD) highlight the emerging need for novel noninvasive markers such as circulating microRNAs (miRNAs). Objective To evaluate the circulating levels of miR-126-3p, miR-210-3p, let-7g-5p, and miR-326, and their associations with known contributors to CAD, in CAD subgroups. Methods We divided the cohort into 4 groups: non-CAD controls (≤30% stenosis; n = 55), and patients with stable angina pectoris (SAP; n = 48), unstable AP (UAP; n = 46), and myocardial infarction (MI; n = 36). The circulating levels of miR-126-3p, miR-210-3p, let-7g-5p, and miR-326 were determined using TaqMan Advanced miRNA Assays in serum specimens. Results Circulating miR-126-3p levels were lower in the MI and UAP groups, compared with the non-CAD group, whereas miR-210-3p circulating levels were lower in the MI group than others. The levels of circulating let-7g-5p were shown to be useful for distinguishing UAP from MI, and there were substantial differences in circulating let-7g-5p levels between the UAP and MI groups. Moreover, lipid levels and ratios were lower in individuals with high circulating miR-126-3p and miR-210-3p levels. Conclusions The study results suggest that circulating miR-126-3p, miR-210-3p, and let-7g-5p are differentiated between different clinical presentations of CAD and associated with lipid levels, which are important risk factors and determinants of CAD.

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Section: Discussionmentioning

confidence: 99%

Alteration of circulating miRNAs during myocardial infarction and association with lipid levels

Ozuynuk-Ertugrul,

Ekici,

Erkan

et al. 2023

Laboratory Medicine

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“…As mentioned above, miR-126 is among the microRNAs implicated in angiogenesis and vascular integrity 11 , 12 . In endothelial cells it inhibits the SDF-1 expression and its silencing elevates SDF-1 levels 13 , 14 .…”

Section: Introductionmentioning

confidence: 95%

“…Furthermore, in endothelial cells cultured in vitro under the hypoxic conditions miR-126 level decreases 15 . In the absence of miR-126 neovascularization associated with myocardial infraction or retinal vascular development and remodeling is distrupted 11 , 12 . The miR-126 is encoded in chromosome 2 (mouse) or 9 (human) within the epidermal growth factor like domain multiple 7 (EGFL7) gene intron.…”

Section: Introductionmentioning

confidence: 99%

miRNA-126a plays important role in myoblast and endothelial cell interaction

Mierzejewski,

Ciemerych,

Streminska

et al. 2023

Sci Rep

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Muscle satellite cells (SCs) are stem cells and the main players in skeletal muscle reconstruction. Since satellite cells are located near or in direct contact with blood vessels their niche is formed, inter alia, by endothelial cells. The cross-talk between satellite cells and endothelial cells determines quiescence or proliferation of these cells. However, little is known about the role of miRNA in these interactions. In the present study we identified miRNA that were up-regulated in SC-derived myoblasts treated with stromal derived factor-1 (SDF-1) and/or down-regulated in cells in which the expression of CXCR4 or CXCR7, that is, SDF-1 receptors, was silenced. SDF-1 is one of the important regulators of cell migration, mobilization, skeletal muscle regeneration, and angiogenesis. We hypothesized that selected miRNAs affect SC-derived myoblast fate and interactions with endothelial cells. We showed that miR-126a-3p inhibited both, myoblast migration and fusion. Moreover, the levels of Cxcl12, encoding SDF-1 and Ackr3, encoding CXCR7, were reduced by miR-126a-3p mimic. Interestingly, the miR-126a-3p mimic significantly decreased the level of numerous factors involved in myogenesis and the miR-126a-5p mimic increased the level of Vefga. Importantly, the treatment of endothelial cells with medium conditioned by miR-126-5p mimic transfected SC-derived myoblasts promoted tubulogenesis.

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“…Another subtype of ncRNAs is the microRNAs (miR-NAs), which have a role in regulating gene expression on post-transcription or post-translation levels [23] and were also found to be altered in CAD patients and implicated in atherosclerosis development [24]. MiRNA-126-5p was among the early discovered miRNAs expressed specifically in the vasculature and have critical functions in vascular development [25]. Recent studies have unveiled the potentially important function of miRNA-126-5p in vascular diseases, including atherosclerosis, coronary artery disease, stroke, and diabetic vasculopathy.…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have unveiled the potentially important function of miRNA-126-5p in vascular diseases, including atherosclerosis, coronary artery disease, stroke, and diabetic vasculopathy. The action of miRNA-126-5p reflects its function in angiogenesis and inflammation [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Introducing Circulating Vasculature-Related Transcripts as Biomarkers in Coronary Artery Disease

et al. 2022

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Background Atherosclerotic plaque is considered the hallmark of atherosclerotic lesions in coronary atherosclerosis (CAS), the primary pathogenesis in coronary artery disease (CAD), which develops and progresses through a complex interplay between immune cells, vascular cells, and endothelial shear stresses. Early diagnosis of CAS is critical for avoiding plaque rupture and sudden death. Therefore, identifying new CAD biomarkers linked to vessel wall functions, such as RNA molecules with their distinct signature, is a promising development for these patients. With this rationale, the present study investigated the expression level of the vascular-related RNA transcripts (lncRNA ANRIL, miRNA-126-5p, CDK4, CDK6, TGF-β, E-cadherin, and TNF-α) implicated in the cellular vascular function, proliferation, and inflammatory processes. Methods A case-control study design with a total of 180 subjects classified participants into two groups; CAD and control groups. The relative expression levels of the seven transcripts under study-selected using online bioinformatics tools and current literature-were assessed in the plasma of all study participants using RT-qPCR. Their predictive significance testing, scoring of disease prioritization, enrichment analysis, and the miRNA-mRNA regulatory network was investigated. ResultsThe relative expression levels of all seven of the circulating vascular-related transcripts under study were statistically significant between CAD patients and controls. Receiver operating characteristic (ROC) analysis results indicated the statistical significance of all the transcripts under study with CDK4 showing the highest area under the curve (AUC) equivalent to 0.91, followed by E-cadherin (0.90), miRNA-126-5p (0.83), ANRIL (0.82), TNF-α (0.63), TGF-β (0.62), and CDK6 (0.59), in descending order. A strong association was detected between most of the transcripts studied in CAD patients with a significant Spearman's correlation coefficient with a two-tailed significance of p < 0.001. Network analysis revealed a strong relationship between the five circulating vasculature transcripts studied and their target miRNAs and miR-126-5p, but not for ANRIL. Conclusion The seven circulating vascular-related RNA transcripts under study could serve as potential CAD biomarkers, reflecting the cellular vascular function, proliferation, and inflammatory processes in CAD patients. Therefore, blood transcriptome analysis opens new frontiers for the non-invasive diagnosis of CAD.

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An integrated hypothesis for miR-126 in vascular disease

Cited by 19 publications

References 22 publications

Alteration of circulating miRNAs during myocardial infarction and association with lipid levels

Alteration of circulating miRNAs during myocardial infarction and association with lipid levels

miRNA-126a plays important role in myoblast and endothelial cell interaction

Introducing Circulating Vasculature-Related Transcripts as Biomarkers in Coronary Artery Disease

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