An integrated micro-chip for rapid detection of magnetic particles

Gooneratne, Chinthaka P.; Cai, Liang; Giouroudi, Ioanna; Kosel, Jürgen

doi:10.1063/1.3678303

Cited by 20 publications

(15 citation statements)

References 19 publications

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“…Therefore, in this research, the emphasis is on the capacity of the MMC to isolate and perform selective treatments on cells tagged with SPBs on a simple and integrated platform. The approach we have taken in this paper differs from our previous work [26][27][28][29][30][31][32][33][34] and from other work in terms of simplicity of fabrication, operation of the device as well as using living cells and also by the integration of all components into a microfluidic chip. Moreover, compared to the commercially available products, the MMC is an integrated chip of small size (3.1 Â 2.3 cm 2 chip area) that enables both cell separation and experiments on-chip.…”

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confidence: 45%

“…F m , calculated for an SPB with a radius of 1.4 lm and a susceptibility of 1.2 46 in a solution with a viscosity of 1 Â 10 À3 PaÁs, has a maximum value of 2.2 pN at the edges of the disk, which is sufficient to trap SPBs. 8,9,13,15,[20][21][22][26][27][28][29][30][31][32][33][34][35]47 Fig . 2(c) shows the velocity of an SPB during the trapping process.…”

Section: -4mentioning

confidence: 99%

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Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

et al. 2014

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This study describes the development and testing of a magnetic microfluidic chip (MMC) for trapping and isolating cells tagged with superparamagnetic beads (SPBs) in a microfluidic environment for selective treatment and analysis. The trapping and isolation are done in two separate steps; first, the trapping of the tagged cells in a main channel is achieved by soft ferromagnetic disks and second, the transportation of the cells into side chambers for isolation is executed by tapered conductive paths made of Gold (Au). Numerical simulations were performed to analyze the magnetic flux and force distributions of the disks and conducting paths, for trapping and transporting SPBs. The MMC was fabricated using standard microfabrication processes. Experiments were performed with E. coli (K12 strand) tagged with 2.8 μm SPBs. The results showed that E. coli can be separated from a sample solution by trapping them at the disk sites, and then isolated into chambers by transporting them along the tapered conducting paths. Once the E. coli was trapped inside the side chambers, two selective treatments were performed. In one chamber, a solution with minimal nutrition content was added and, in another chamber, a solution with essential nutrition was added. The results showed that the growth of bacteria cultured in the second chamber containing nutrient was significantly higher, demonstrating that the E. coli was not affected by the magnetically driven transportation and the feasibility of performing different treatments on selectively isolated cells on a single microfluidic platform.

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confidence: 45%

Section: -4mentioning

confidence: 99%

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

et al. 2014

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“…It is a quantum mechanical effect based on spin dependent scattering in magnetic multilayers. Sensors based on this effect have received increasing interest for biomedical applications because they have been proven to exhibit higher sensitivity at low fields than other MR based sensors, thus being more suitable for the detection of biological target species by means of immunomagnetic assays in combination with MNPs [1,33,35,38–41]. GMR structures have an advantage in size, power consumption, cost and thermal stability with respect to search coil, fluxgate, SQUID, Hall and spin resonance sensors.…”

Section: Introductionmentioning

confidence: 99%

“…GMR structures have an advantage in size, power consumption, cost and thermal stability with respect to search coil, fluxgate, SQUID, Hall and spin resonance sensors. Furthermore, GMR sensors are also ideal for low cost applications since they are easily energized by applying a constant current and the output voltage is a measure of the magnetic field [33]. …”

Section: Introductionmentioning

confidence: 99%

Microfluidic Biosensing Systems Using Magnetic Nanoparticles

Giouroudi

Keplinger

2013

IJMS

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In recent years, there has been rapidly growing interest in developing hand held, sensitive and cost-effective on-chip biosensing systems that directly translate the presence of certain bioanalytes (e.g., biomolecules, cells and viruses) into an electronic signal. The impressive and rapid progress in micro- and nanotechnology as well as in biotechnology enables the integration of a variety of analytical functions in a single chip. All necessary sample handling and analysis steps are then performed within the chip. Microfluidic systems for biomedical analysis usually consist of a set of units, which guarantees the manipulation, detection and recognition of bioanalytes in a reliable and flexible manner. Additionally, the use of magnetic fields for performing the aforementioned tasks has been steadily gaining interest. This is because magnetic fields can be well tuned and applied either externally or from a directly integrated solution in the biosensing system. In combination with these applied magnetic fields, magnetic nanoparticles are utilized. Some of the merits of magnetic nanoparticles are the possibility of manipulating them inside microfluidic channels by utilizing high gradient magnetic fields, their detection by integrated magnetic microsensors, and their flexibility due to functionalization by means of surface modification and specific binding. Their multi-functionality is what makes them ideal candidates as the active component in miniaturized on-chip biosensing systems. In this review, focus will be given to the type of biosening systems that use microfluidics in combination with magnetoresistive sensors and detect the presence of bioanalyte tagged with magnetic nanoparticles.

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“…A long-range displacement of magnetic particles can be assured by arranging adjacent ring type conductors in an array with spatial overlap. [28][29][30][31] A 3D model was necessary for simulating the magnetophoretic capability of this type of circular conductors. As illustrated in Fig.…”

Section: A Simulationsmentioning

confidence: 99%

On-chip microfluidic biosensor using superparamagnetic microparticles

Keplinger

Giouroudi

2013

Biomicrofluidics

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In this paper, an integrated solution towards an on-chip microfluidic biosensor using the magnetically induced motion of functionalized superparamagnetic microparticles (SMPs) is presented. The concept of the proposed method is that the induced velocity on SMPs in suspension, while imposed to a magnetic field gradient, is inversely proportional to their volume. Specifically, a velocity variation of suspended functionalized SMPs inside a detection microchannel with respect to a reference velocity, specified in a parallel reference microchannel, indicates an increase in their non-magnetic volume. This volumetric increase of the SMPs is caused by the binding of organic compounds (e.g., biomolecules) to their functionalized surface. The new compounds with the increased non-magnetic volume are called loaded SMPs (LSMPs). The magnetic force required for the manipulation of the SMPs and LSMPs is produced by current currying conducting microstructures, driven by a programmable microcontroller. Experiments were carried out as a proof of concept. A promising decrease in the velocity of the LSMPs in comparison to that of the SMPs was measured. Thus, it is the velocity variation which determines the presence of the organic compounds in the sample fluid. V C 2013 AIP Publishing LLC. [http://dx

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An integrated micro-chip for rapid detection of magnetic particles

Cited by 20 publications

References 19 publications

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

Microfluidic Biosensing Systems Using Magnetic Nanoparticles

On-chip microfluidic biosensor using superparamagnetic microparticles

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