An integrated microfluidic concentration gradient generator for mechanical stimulation and drug delivery

Shourabi, Arash Yahyazadeh; Kashaninejad, Navid; Saidi, Mohammad R.

doi:10.1016/j.jsamd.2021.02.009

Cited by 39 publications

(36 citation statements)

References 47 publications

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“…The selection of the geometry for gradient generation must be considered carefully to produce an appropriate shear environment. These selections may be made by proper numerical simulation studies [107]. The simulation is helpful to determine the gradient and shear rate for individual sections with different geometry.…”

Section: High-throughput Challengesmentioning

confidence: 99%

Multi-Organs-on-Chips for Testing Small-Molecule Drugs: Challenges and Perspectives

et al. 2021

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Organ-on-a-chip technology has been used in testing small-molecule drugs for screening potential therapeutics and regulatory protocols. The technology is expected to boost the development of novel therapies and accelerate the discovery of drug combinations in the coming years. This has led to the development of multi-organ-on-a-chip (MOC) for recapitulating various organs involved in the drug–body interactions. In this review, we discuss the current MOCs used in screening small-molecule drugs and then focus on the dynamic process of drug absorption, distribution, metabolism, and excretion. We also address appropriate materials used for MOCs at low cost and scale-up capacity suitable for high-performance analysis of drugs and commercial high-throughput screening platforms.

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Section: High-throughput Challengesmentioning

confidence: 99%

Multi-Organs-on-Chips for Testing Small-Molecule Drugs: Challenges and Perspectives

et al. 2021

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“…Polymer-based devices are mainly built from polydimethylsiloxane (PDMS) using soft lithography techniques. Some PDMS-based microchips take advantage of concentration gradient generators (CGGs) [23,29,30], while others set drug dosage manually on a microwell platform or automatically by diffusivity of the reagents. Moreover, they can culture cells 2D or 3D.…”

Section: Monodrug Screening On a Microscale Samplementioning

confidence: 99%

“…A microarray spotter dispensed the drug solutions onto the microwell chip. Figure 1c is a two-layer PDMS-based microfluidic device with an integrated cell culture membrane that can be used for both drug delivery and mechanotransduction [23]. It should be noted that the size of the membrane can be tailored based on the sample size.…”

Section: Monodrug Screening On a Microscale Samplementioning

confidence: 99%

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Anti-Cancer Drug Screening with Microfluidic Technology

Monjezi¹,

Rismanian²,

Jamaati³

et al. 2021

Preprint

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The up-and-coming microfluidic technology is the most promising platform for designing anti-cancer drugs and new point-of-care diagnostics. Compared to conventional drug screening methods based on Petri dishes and animal studies, drug delivery in microfluidic systems has many advantages. For instance, these platforms offer high throughput drug screening, require a small amount of samples, provide an in vivo-like microenvironment for cells, and eliminate ethical issues associated with animal studies. Multiple cell cultures in microfluidic chips could better mimic the 3D tumor environment using low reagents consumption. The clinical experiments have shown that combinatorial drug treatments have a better therapeutic effect than monodrug therapy. So many attempts were performed in this field in the last decade. This review highlights the applications of microfluidic chips in anti-cancer drug screening and systematically categorizes these systems as a function of sample size and combination of drug screening. Finally, it provides a perspective on the future of the clinical applications of microfluidic systems for anti-cancer drug development.

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“…Such systems are increasingly being used to mimic the different key phenomena of the tumor micro-environment, such as aggregation, formation, growth, angiogenesis, and metastasis of cancer tumor cells [ 15 , 16 ]. In addition, hydro-dynamically trapping the cells in these devices leads to highly uniform size distributions of spheroids [ 17 ], since the fluidic conditions in microchannels are precisely adjustable and feasibly controllable [ 18 ]. One of the most common techniques used to generate and culture these spheroids on microfluidic chips is the integration of microchannels and microwells [ 15 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

A Proof-of-Concept Study Using Numerical Simulations of an Acoustic Spheroid-on-a-Chip Platform for Improving 3D Cell Culture

Shourabi

Salajeghe

Barisam

et al. 2021

Sensors

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Microfluidic lab-on-chip devices are widely being developed for chemical and biological studies. One of the most commonly used types of these chips is perfusion microwells for culturing multicellular spheroids. The main challenge in such systems is the formation of substantial necrotic and quiescent zones within the cultured spheroids. Herein, we propose a novel acoustofluidic integrated platform to tackle this bottleneck problem. It will be shown numerically that such an approach is a potential candidate to be implemented to enhance cell viability and shrinks necrotic and quiescent zones without the need to increase the flow rate, leading to a significant reduction in costly reagents’ consumption in conventional spheroid-on-a-chip platforms. Proof-of-concept, designing procedures and numerical simulation are discussed in detail. Additionally, the effects of acoustic and hydrodynamic parameters on the cultured cells are investigated. The results show that by increasing acoustic boundary displacement amplitude (d0), the spheroid’s proliferating zone enlarges greatly. Moreover, it is shown that by implementing d0= 0.5 nm, the required flow rate to maintain the necrotic zone below 13% will be decreased 12 times compared to non-acoustic chips.

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An integrated microfluidic concentration gradient generator for mechanical stimulation and drug delivery

Cited by 39 publications

References 47 publications

Multi-Organs-on-Chips for Testing Small-Molecule Drugs: Challenges and Perspectives

Multi-Organs-on-Chips for Testing Small-Molecule Drugs: Challenges and Perspectives

Anti-Cancer Drug Screening with Microfluidic Technology

A Proof-of-Concept Study Using Numerical Simulations of an Acoustic Spheroid-on-a-Chip Platform for Improving 3D Cell Culture

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