An integrated microfluidic platform for detection of ovarian clear cell carcinoma mRNA biomarker FXYD2

Chung, Yi Da; Liu, Ting Hang; Liang, Yu; Lin, Chang Ching; Hsu, Keng Fu; Lee, Gwo‐Bin

doi:10.1039/d1lc00177a

Cited by 5 publications

(3 citation statements)

References 42 publications

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“…Using microfluidic chip technology to screen the specific binding of oligopeptides to ovarian cancer cells is also an effective strategy for early diagnosis of ovarian cancer [ 270 ]. Additionally, high expression of the FXYD2 gene is closely related to ovarian clear cell carcinoma, which is a highly lethal ovarian cancer subtype, and the use microfluidic chip can achieve rapid, sensitive, specific and quantitative detection of the FXYD2 gene [ 271 ]. Such systems have shown great promise in enabling the simulation and mechanism analysis of ovarian cancer microenvironments and facilitating early detection and improved management of this devastating disease.…”

Section: Microfluidic Chips For Gynecological Diseasesmentioning

confidence: 99%

Revolutionizing the female reproductive system research using microfluidic chip platform

Yan,

Wu,

Zhang

et al. 2023

J Nanobiotechnol

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Comprehensively understanding the female reproductive system is crucial for safeguarding fertility and preventing diseases concerning women's health. With the capacity to simulate the intricate physio- and patho-conditions, and provide diagnostic platforms, microfluidic chips have fundamentally transformed the knowledge and management of female reproductive health, which will ultimately promote the development of more effective assisted reproductive technologies, treatments, and drug screening approaches. This review elucidates diverse microfluidic systems in mimicking the ovary, fallopian tube, uterus, placenta and cervix, and we delve into the culture of follicles and oocytes, gametes’ manipulation, cryopreservation, and permeability especially. We investigate the role of microfluidics in endometriosis and hysteromyoma, and explore their applications in ovarian cancer, endometrial cancer and cervical cancer. At last, the current status of assisted reproductive technology and integrated microfluidic devices are introduced briefly. Through delineating the multifarious advantages and challenges of the microfluidic technology, we chart a definitive course for future research in the woman health field. As the microfluidic technology continues to evolve and advance, it holds great promise for revolutionizing the diagnosis and treatment of female reproductive health issues, thus propelling us into a future where we can ultimately optimize the overall wellbeing and health of women everywhere. Graphical Abstract

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Section: Microfluidic Chips For Gynecological Diseasesmentioning

confidence: 99%

Revolutionizing the female reproductive system research using microfluidic chip platform

Yan,

Wu,

Zhang

et al. 2023

J Nanobiotechnol

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“…There are usually two solutions: first, develop a one-pot CRISPR/Cas-assisted RPA detection method, but few previously one-pot CRISPR-RAA assays only generated weak signals for the low-concentration sample test even if it takes a long time. , The main reason is that the Cas12a nuclease consumes amplicons and reduces amplification efficiency. , Thus, the second strategy, separating the amplification from the CRISPR/Cas readout, might be more valuable than the one-pot strategy . However, developing a completely automated POC test (POCT) platform that includes both steps is still quite a challenge. − …”

Section: Introductionmentioning

confidence: 99%

Dual-CRISPR/Cas12a-Assisted RT-RAA for Ultrasensitive SARS-CoV-2 Detection on Automated Centrifugal Microfluidics

Chen

Zong

et al. 2022

Anal. Chem.

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The clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid detection can be combined with recombinase-aided amplification (RAA) to enable rapid, accurate, and early detection of SARS-CoV-2. Current CRISPR-based approaches to detecting viral nucleic acid typically require immense manual operations to transfer RPA amplicons for CRISPR detection or suffer from compromised sensitivity by mixing the competing RPA amplification and CRISPR detection. Here, we develop dual-CRISPR/Cas12a-assisted RT-RAA assay and a ″sample-to-answer″ centrifugal microfluidic platform that can automatically detect 1 copy/μL of the SARS-CoV-2 within 30 min. This chip separates the amplification (RAA) from detection (CRISPR), such that sensitivity is maximized and the time consumption is decreased by a factor of 3. For the 26 positive and 8 negative clinical SARS-CoV-2 samples, this automated centrifugal microfluidics achieved 100% accuracy compared to the gold-standard RT-PCR technique. This point-of-care test, with the advantages of being one-step, automated, rapid, and sensitive, will have a significant potential for clinical diagnosis and disease prevention.

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“…Today, many papers have reported the integration of microfluidics with electronic sensors. Sensor-integrated microfluidic devices were reported for applications such as DNA hybridization [ 28 , 29 ], polymerase chain reaction (PCR) on-chip [ 30 , 31 ], virus detection [ 32 , 33 ], cancer detection [ 34 , 35 ], circulating tumor cells (CTC) analysis [ 36 , 37 ], exosomes analysis [ 38 , 39 ], nucleic acids analysis [ 40 , 41 ], liquid biopsy-based assays for cancer detection [ 42 ]. CMOS-based sensors integration with microfluidic is put forward for cell analysis [ 43 , 44 ], chemical sensing [ 45 ], optical biosensing [ 46 ], ultraprecise micro pumping [ 47 ], 2D nanomaterial detection [ 48 ], optofluidics biomedical devices [ 49 ].…”

Section: Introductionmentioning

confidence: 99%

A Hybrid Microfluidic Electronic Sensing Platform for Life Science Applications

Panahi

Ghafar-Zadeh

2022

Micromachines

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This paper presents a novel hybrid microfluidic electronic sensing platform, featuring an electronic sensor incorporated with a microfluidic structure for life science applications. This sensor with a large sensing area of 0.7 mm2 is implemented through a foundry process called Open-Gate Junction FET (OG-JFET). The proposed OG-JFET sensor with a back gate enables the charge by directly introducing the biological and chemical samples on the top of the device. This paper puts forward the design and implementation of a PDMS microfluidic structure integrated with an OG-JFET chip to direct the samples toward the sensing site. At the same time, the sensor’s gain is controlled with a back gate electrical voltage. Herein, we demonstrate and discuss the functionality and applicability of the proposed sensing platform using a chemical solution with different pH values. Additionally, we introduce a mathematical model to describe the charge sensitivity of the OG-JFET sensor. Based on the results, the maximum value of transconductance gain of the sensor is ~1 mA/V at Vgs = 0, which is decreased to ~0.42 mA/V at Vgs = 1, all in Vds = 5. Furthermore, the variation of the back-gate voltage from 1.0 V to 0.0 V increases the sensitivity from ~40 mV/pH to ~55 mV/pH. As per the experimental and simulation results and discussions in this paper, the proposed hybrid microfluidic OG-JFET sensor is a reliable and high-precision measurement platform for various life science and industrial applications.

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An integrated microfluidic platform for detection of ovarian clear cell carcinoma mRNA biomarker FXYD2

Abstract: In this work we developed an integrated microfluidic system for automatically detecting the ovarian clear cell carcinoma (OCCC) biomarker FXYD2. Capture of cancer cells, isolation of messenger RNA, and quantitative...

Cited by 5 publications

References 42 publications

Revolutionizing the female reproductive system research using microfluidic chip platform

Revolutionizing the female reproductive system research using microfluidic chip platform

Dual-CRISPR/Cas12a-Assisted RT-RAA for Ultrasensitive SARS-CoV-2 Detection on Automated Centrifugal Microfluidics

A Hybrid Microfluidic Electronic Sensing Platform for Life Science Applications

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