An integrated microfluidic system for live bacteria detection from human joint fluid samples by using ethidium monoazide and loop-mediated isothermal amplification

Chen, Shu Ling; Chang, Wen Hsin; Wang, Chih‐Hung; You, Huey Ling; Wu, Jiunn Jong; Liu, Ting Hang; Lee, Mel S.; Lee, Gwo‐Bin

doi:10.1007/s10404-017-1913-8

Cited by 15 publications

(8 citation statements)

References 32 publications

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“…While DNA and immunoassays are sensitive, selective and faster than classic culture-based methods, they presume costly instrumentation, significant cost per assay, possibly suffer from interferences from real samples, and/or cannot differentiate live/dead bacteria, all of which prevent their application on a wider scale. There was a significant progress in development of microfluidic devices with complex capabilities for bacteria isolation, cell lysis and DNA amplification for identifying live bacteria [ 87 ]. Polymerase chain reaction (PCR), [ 88 ] dielectrophoresis [ 89 , 90 ], and Surface Enhanced Raman Spectroscopy (SERS) [ 91 ] are among the other modern techniques that have been integrated with microchips or biosensors.…”

Section: Evaluation Of Antibacterial Activity With Methods Using Smentioning

confidence: 99%

Detection of Antibiotics and Evaluation of Antibacterial Activity with Screen-Printed Electrodes

Munteanu

Titoiu

Marty

et al. 2018

Sensors

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This review provides a brief overview of the fabrication and properties of screen-printed electrodes and details the different opportunities to apply them for the detection of antibiotics, detection of bacteria and antibiotic susceptibility. Among the alternative approaches to costly chromatographic or ELISA methods for antibiotics detection and to lengthy culture methods for bacteria detection, electrochemical biosensors based on screen-printed electrodes present some distinctive advantages. Chemical and (bio)sensors for the detection of antibiotics and assays coupling detection with screen-printed electrodes with immunomagnetic separation are described. With regards to detection of bacteria, the emphasis is placed on applications targeting viable bacterial cells. While the electrochemical sensors and biosensors face many challenges before replacing standard analysis methods, the potential of screen-printed electrodes is increasingly exploited and more applications are anticipated to advance towards commercial analytical tools.

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Section: Evaluation Of Antibacterial Activity With Methods Using Smentioning

confidence: 99%

Detection of Antibiotics and Evaluation of Antibacterial Activity with Screen-Printed Electrodes

Munteanu

Titoiu

Marty

et al. 2018

Sensors

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“…For the next advance, microfluidic-based techniques have emerged in recent years as an approach to detect biological matter in fluidic samples [58,59]. These techniques allow for miniaturized solutions with a lab-on-chip approach with reduced costs and high resolution.…”

Section: Water Sample Analysesmentioning

confidence: 99%

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

et al. 2021

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The main goal of this study was to apply magnetic bead surface functionalization in the form of immunomagnetic separation (IMS) combined with real-time polymerase chain reaction (qPCR) (IMS-qPCR) to detect Human mastadenovirus species C (HAdV-C) and F (HAdV-F) in water samples. The technique efficiency was compared to a nonfunctionalized method (ultracentrifugation) followed by laboratory detection. Tests were carried out to standardize IMS parameters followed by tests on 15 water samples concentrated by IMS and ultracentrifugation. Microscopic analyses detected a successful beads–antibody attachment. HAdV was detected up to dilutions of 10−6 by IMS-qPCR, and samples concentrated by IMS were able to infect cell cultures. In water samples, HAdV-C was detected in 60% (monoclonal) and 47% (polyclonal) by IMS-qPCR, while 13% of samples concentrated by ultracentrifugation gave a positive result. HAdV-F was positive in 27% of samples by IMS-qPCR (polyclonal) and ultracentrifugation and 20% by IMS-qPCR (monoclonal). The rate of detection varied from 4.55 × 102 to 5.83 × 106 genomic copies/L for IMS-qPCR and from 2.00 × 102 to 2.11 × 103 GC/L for ultracentrifugation. IMS showed to be a more effective concentration technique for HAdV than ultracentrifugation, improving the assessment of infectious HAdV in water resources.

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“…Since the EMA cannot enter live cellular membranes, it can be used for labelling the DNA of dead cells within a population of viable and dead cells [216]. This marker has been used in various integrated amplification microfluidics to detect bacteria such as methicillin-resistant S. aureus (MRSA) [216], E. coli , S. aureus , P. syringae , Enterococcus sp., methicillin-resistant S. aureus , and coagulase-negative staphylococci [217], and live bacteria for periprosthetic joint infection [218].…”

Section: Microfluidic Sample Preparation For Amplificationmentioning

confidence: 99%

Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology

2019

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Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. Novel and innovative strategies for detection and identification of bacterial nucleic acids are important for practical applications. Microfluidics is an emerging technology that only requires small amounts of liquid samples. Microfluidic devices allow for rapid advances in microbiology, enabling access to methods of amplifying nucleic acid molecules and overcoming difficulties faced by conventional. In this review, we summarize the recent progress in microfluidics-based polymerase chain reaction devices for the detection of nucleic acid biomarkers. The paper also discusses the recent development of isothermal nucleic acid amplification and droplet-based microfluidics devices. We discuss recent microfluidic techniques for sample preparation prior to the amplification process.

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An integrated microfluidic system for live bacteria detection from human joint fluid samples by using ethidium monoazide and loop-mediated isothermal amplification

Cited by 15 publications

References 32 publications

Detection of Antibiotics and Evaluation of Antibacterial Activity with Screen-Printed Electrodes

Detection of Antibiotics and Evaluation of Antibacterial Activity with Screen-Printed Electrodes

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology

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