An integrated microfluidic system with field-effect-transistor sensor arrays for detecting multiple cardiovascular biomarkers from clinical samples

Sinha, Anirban; Tai, Tse Yu; Li, Kuang Hsien; Gopinathan, Priya; Chung, Yi Da; Sarangadharan, Indu; Pin, Hsi; Huang, Po Chiun; Shiesh, Shu Chu; Wang, Yu Lin; Lee, Gwo‐Bin

doi:10.1016/j.bios.2019.01.001

Cited by 83 publications

(51 citation statements)

References 47 publications

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“…The latest assays achieve very high sensitivity in order to avoid false negatives as much as possible. However, they still have drawbacks such as high cost, long processing times [3,105], and a lack of standardization [164]. Researchers are thus working on solutions to provide better sensitivity, user-friendliness, and which can be competitive with previous solutions in terms of size and cost.…”

Section: Challenges and Emerging Trends In Cardiac Biomarkers Detectionmentioning

confidence: 99%

“…In order to improve the specificity of actual assays for AMI diagnosis, the first solution is the combination of multiple cardiac biomarkers analysis. Indeed, multiplexed assays provide more information than measurement of a single biomarker, and thus can substantially help for diagnosis [3,4,12,16,83,164]. For instance, a study conducted by Wang et al, shows that parallel measurements of high-sensitivity cTnI (hs-cTnI) and NT-proBNP are more reliable for AMI diagnosis than a hs-cTnI assay alone [28].…”

Section: Multiplexing Assays For a Better Specificitymentioning

confidence: 99%

“…This technology has several advantages including rapidity, high sensitivity, reliability, small size and low-energy consumption that make it idealfor POC diagnosis. This portable system, based on a microfluidic platform and a FET sensor, can process an automated detection of cTnI, NT-proBNP, CRP and fibrinogen in 4 µL of clinical samples in 5 min [3].…”

Section: Multiplexing Assays For a Better Specificitymentioning

confidence: 99%

“…Indeed, the blood concentration of several specific cardiac biomarkers evolves over time during a myocardial infarction. Hence, their measurements can be used to improve the diagnosis [3,7,12,20,26,31,40]. Concerning the biomarker assessment, one measurement value should be higher than the 99 th percentile of the reference population to confirm the diagnosis [34,38,41].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Recent advances in cardiac biomarkers detection: From commercial devices to emerging technologies

Savonnet

Rolland

Cubizolles

et al. 2021

Journal of Pharmaceutical and Biomedical Analysis

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Section: Challenges and Emerging Trends In Cardiac Biomarkers Detectionmentioning

confidence: 99%

Section: Multiplexing Assays For a Better Specificitymentioning

confidence: 99%

Section: Multiplexing Assays For a Better Specificitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Recent advances in cardiac biomarkers detection: From commercial devices to emerging technologies

Savonnet

Rolland

Cubizolles

et al. 2021

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

“…However, this kind of driving manner cannot actively control the flow and the unidirectional flow also hinders its reaction efficiency. Hu et al [49] described a self-contained and portable microfluidic chemiluminescence immune sensor for quantitative detection of biomarkers. The microfluidic device contains different reagents in individual reservoirs, which are isolated by on-chip mechanical valves.…”

Section: Introductionmentioning

confidence: 99%

A Portable Microfluidic System for Point-of-Care Detection of Multiple Protein Biomarkers

Mengyu

2021

Micromachines

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Protein biomarkers are indicators of many diseases and are commonly used for disease diagnosis and prognosis prediction in the clinic. The urgent need for point-of-care (POC) detection of protein biomarkers has promoted the development of automated and fully sealed immunoassay platforms. In this study, a portable microfluidic system was established for the POC detection of multiple protein biomarkers by combining a protein microarray for a multiplex immunoassay and a microfluidic cassette for reagent storage and liquid manipulation. The entire procedure for the immunoassay was automatically conducted, which included the antibody–antigen reaction, washing and detection. Alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carcinoma antigen 125 (CA125) were simultaneously detected in this system within 40 min with limits of detection of 0.303 ng/mL, 1.870 ng/mL, and 18.617 U/mL, respectively. Five clinical samples were collected and tested, and the results show good correlations compared to those measured by the commercial instrument in the hospital. The immunoassay cassette system can function as a versatile platform for the rapid and sensitive multiplexed detection of biomarkers; therefore, it has great potential for POC diagnostics.

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Integrated Electrochemical and Optical Biosensing in Organs‐on‐Chip

Tawade,

Mastrangeli

2023

ChemBioChem

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Demand for biocompatible, non‐invasive, and continuous real‐time monitoring of organs‐on‐chip has driven the development of a variety of novel sensors. However, highest accuracy and sensitivity can arguably be achieved by integrated biosensing, which enables in situ monitoring of the in vitro microenvironment and dynamic responses of tissues and miniature organs recapitulated in organs‐on‐chip. This paper reviews integrated electrical, electrochemical, and optical sensing methods within organ‐on‐chip devices and platforms. By affording precise detection of analytes and biochemical reactions, these methods expand and advance the monitoring capabilities and reproducibility of organ‐on‐chip technology. The integration of these sensing techniques allows a deeper understanding of organ functions, and paves the way for important applications such as drug testing, disease modeling, and personalized medicine. By consolidating recent advancements and highlighting challenges in the field, this review aims to foster further research and innovation in the integration of biosensing in organs‐on‐chip.

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An integrated microfluidic system with field-effect-transistor sensor arrays for detecting multiple cardiovascular biomarkers from clinical samples

Cited by 83 publications

References 47 publications

Recent advances in cardiac biomarkers detection: From commercial devices to emerging technologies

Recent advances in cardiac biomarkers detection: From commercial devices to emerging technologies

A Portable Microfluidic System for Point-of-Care Detection of Multiple Protein Biomarkers

Integrated Electrochemical and Optical Biosensing in Organs‐on‐Chip

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