An Integrated Multifunctional Lab-on-a-Chip Platform for High Throughput Optical Mapping of DNA

Kwok, Lisa W.; Yi, Zili; Crane, Bryan; Knaian, Linda; Vyavahare, Kedar; Meltzer, Robert H.; Griffis, Joshua W.; Edmonson, Amanda; Zhong, Qun; Allen, R. E.; Mesadieu, Wolf; Larson, Jonathan W.; Krogmeier, Jeffrey R.; Gilmanshin, Rudolf

doi:10.1016/j.bpj.2008.12.142

Cited by 1 publication

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“…In this experiment, a 20 ml reaction was processed using an integrated microfluidic chip that combines DNA stretching, cleanup with a DNA prism, and concentration on acrylamide gel surfaces. 28 Details of this device will be published elsewhere. The prism consists of a hexagonal array of posts that interact with long DNA molecules as they are pulled through the device with an alternating pair of electric fields (Fig.…”

Section: Assay Sensitivitymentioning

confidence: 99%

A microfluidic chip-compatible bioassay based on single-molecule detection with high sensitivity and multiplexing

et al. 2010

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Many applications in pharmaceutical development, clinical diagnostics, and biological research demand rapid detection of multiple analytes (multiplexed detection) in a minimal volume. This need has led to the development of several novel array-based sensors. The most successful of these so far have been suspension arrays based on polystyrene beads. However, the 5 microm beads used for these assays are incompatible with most microfluidic chip technologies, mostly due to clogging problems. The challenge, then, is to design a detection particle that has high information content (for multiplexed detection), is compatible with miniaturization, and can be manufactured easily at low cost. DNA is a solid molecular wire that is easily produced and manipulated, which makes it a useful material for nanoparticles. DNA molecules are very information-rich, readily deformable, and easily propagated. We exploit these attributes in a suspension array sensor built from specialized recombinant DNA, Digital DNA, that carries both specific analyte-recognition units, and a geometrically encoded identification pattern. Here we show that this sensor combines high multiplexing with high sensitivity, is biocompatible, and has sufficiently small particle size to be used within microfluidic chips that are only 1 microm deep. We expect this technology will be the foundation of a broadly applicable technique to identify and quantitate proteins, nucleic acids, viruses, and toxins simultaneously in a minimal volume.

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Section: Assay Sensitivitymentioning

confidence: 99%