An Integrated Nitric Oxide Sensor Based on Carbon Fiber Coated with Selective Membranes

Zhang, Xueji; Cardosa, Levis; Broderick, Mark; Fein, Harry; Lin, Jun

doi:10.1002/1521-4109(200010)12:14<1113::aid-elan1113>3.0.co;2-u

Cited by 70 publications

(44 citation statements)

References 23 publications

(8 reference statements)

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“…Therefore, further surface modification with permselective membranes is required to achieve the desired selectivity for NO via size exclusion or electrostatic repulsion [4]. Indeed, several polymeric materials have been evaluated as gaspermeable or permselective membranes including nafion, collodion, polycarbazole, o-and m-phenylenediamine, poly(tetrafluoroethylene), cellulose acetate, and multilayer hybrids of these polymers [27,[30][31][32][33][34][35][36]. In particular, Shin et al reported that sol-gel-derived electrochemical sensors showed good sensitivity and selectivity for NO detection [17,27].…”

Section: Direct Methods (Nitric Oxide Sensor)mentioning

confidence: 99%

Real-Time Monitoring of Nitric Oxide Dynamics in the Myocardium: Biomedical Application of Nitric Oxide Sensor

Lee¹,

Lee²,

Park³

2017

Nitric Oxide Synthase - Simple Enzyme-Complex Roles

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Nitric oxide (NO) is an important physiological mediator that regulates a wide range of cellular processes in many tissues. Therefore, the accurate and reliable measurement of physiological NO concentration is essential to the understanding of NO signaling and its biological role. Most methods used for NO detection are indirect including spectroscopic approaches such as the Griess assay for nitrite and detection of methemoglobin after NO reaction with oxyhemoglobin. These methods cannot accurately reflect the changes in NO concentration in vivo and in real time. Therefore, direct methods are necessary for investigating biological process and diseases related to NO in biological conditions. There is a growing interest in the development of electrochemically based sensors for direct, in vivo, and real-time monitoring of NO. Electrochemical methods offer simplicity, good sensitivity, high selectivity, fast response times, and long-term calibration stability compared to other techniques including electron paramagnetic resonance, chemiluminescence, and fluorescence. In this article, we present real-time NO dynamics in the myocardium during myocardial ischemia-reperfusion (IR) utilizing electrochemical NO microsensor. And applications of electrochemical NO sensor for the evaluation of cardioprotective effects of therapeutic treatments such as drug administration and ischemic preconditioning are reviewed.

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Section: Direct Methods (Nitric Oxide Sensor)mentioning

confidence: 99%

Real-Time Monitoring of Nitric Oxide Dynamics in the Myocardium: Biomedical Application of Nitric Oxide Sensor

Lee¹,

Lee²,

Park³

2017

Nitric Oxide Synthase - Simple Enzyme-Complex Roles

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“…The basic electrode design, and details of its performance have already been published [1,9]. Electrodes, specially modified for our in vivo use, were constructed ( Figure 1), with glass insulation to absolutely preclude NO reactivity.…”

Section: Structure Of the No Electrode: (World Precision Instruments mentioning

confidence: 99%

“…Accordingly, we modified the WPI amperometric NO electrode (WPI P/N ISO-NOP007), with an integrated Ag/AgCl reference system, so that its reactive length is approximately 5-15 µm and could be completely inserted into a tubule (Figure 1). The basics of this technique, and the specificity, sensitivity, and other electrochemical characteristics have already been described by Zhang et al [9]. The purpose of the present review is to describe, for the non-kidney investigator, the technique of real time in vivo measurements, with emphasis on calibration issues, and precautions related to detecting integrity of the electrode membrane during its traumatic in vivo use.…”

Section: Introductionmentioning

confidence: 96%

Real Time Microelectrode Measurement of Nitric Oxide in Kidney Tubular Fluid in vivo

Levine

Iacovitti

2003

Sensors

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Abstract:In this review we summarize our experience using a microelectrode to measure nitric oxide concentrations [NO] in living rat kidney tubules. In the anaesthetized living rat, the abdomen can be opened, and the kidney can be placed in a cup such that one can puncture a surface single tubular segment, 1-2 mm long, connected to one of 30,000 filtering glomeruli. The tubular segment can be viewed with a stereo microscope and punctured using sophisticated micromanipulators. The segment, ranging in diameter from about 15 -35 um contains freely flowing RBC-free fluid, electrolytes, O 2 , pCO 2 and NO gas concentrations, and a host of other known and unknown substances. After a "pre" puncture with a 7-10 um beveled glass pipette, intratubular [NO] can be directly determined by inserting, into the tubular lumen, the tip of a specially modified amperometric integrated electrode (WPI P/N ISO-NOP007). We review our in vivo experience with this electrode, emphasizing optimal practice to ensure appropriate calibration, stability, and selectivity for in vivo use. The electrode is highly selective for NO, and, despite fragility, with appropriate precautions, it can provide reproducible and highly sensitive NO measurements in the 40-1000 nM range.

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“…The first method involves the preparation of NO standard solutions from pure NO gas obtained by buying a commercial cylinder with a stainless-steel regulator. However, although this is the most commonly used method for NO sensor calibration found in the literature [16][17][18][19][20][21][22], it suffers from a number of drawbacks including expense and shelf-life.…”

Section: Introductionmentioning

confidence: 99%

“…This method of NO calibration is generally accepted as being suitable for most NO sensors [25][26][27][28][29]. However, SNAP does have drawbacks relating to its stability and purity, because it is extremely sensitive to light and temperature [21,25,30,31].…”

Section: Introductionmentioning

confidence: 99%

Calibration of NO sensors for in-vivo voltammetry: laboratory synthesis of NO and the use of UV?visible spectroscopy for determining stock concentrations

et al. 2005

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The increasing scientific interest in nitric oxide (NO) necessitates the development of novel and simple methods of synthesising NO on a laboratory scale. In this study we have refined and developed a method of NO synthesis, using the neutral Griess reagent, which is inexpensive, simple to perform, and provides a reliable method of generating NO gas for in-vivo sensor calibration. The concentration of the generated NO stock solution was determined using UV-visible spectroscopy to be 0.28±0.01 mmol L À1 . The level of NO 2 À contaminant, also determined using spectroscopy, was found to be 0.67±0.21 mmol L À1 . However, this is not sufficient to cause any considerable increase in oxidation current when the NO stock solution is used for electrochemical sensor calibration over physiologically relevant concentrations; the NO sensitivity of bare Pt-disk electrodes operating at +900 mV (vs. SCE) was 1.08 nA lmol À1 L, while that for NO 2 À was 5.9·10 À3 nA lmol À1 L. The stability of the NO stock solution was also monitored for up to 2 h after synthesis and 30 min was found to be the time limit within which calibrations should be performed.

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An Integrated Nitric Oxide Sensor Based on Carbon Fiber Coated with Selective Membranes

Cited by 70 publications

References 23 publications

Real-Time Monitoring of Nitric Oxide Dynamics in the Myocardium: Biomedical Application of Nitric Oxide Sensor

Real-Time Monitoring of Nitric Oxide Dynamics in the Myocardium: Biomedical Application of Nitric Oxide Sensor

Real Time Microelectrode Measurement of Nitric Oxide in Kidney Tubular Fluid in vivo

Calibration of NO sensors for in-vivo voltammetry: laboratory synthesis of NO and the use of UV?visible spectroscopy for determining stock concentrations

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