Decreased stand uniformity together with reduced aesthetics and playability caused by annual bluegrass (Poa annua) intrusion in creeping bentgrass (Agrostis stolonifera) putting greens is one of the major problems that golf course superintendents face with managing newer playing surfaces. Few herbicides are registered for selective control of annual bluegrass in creeping bentgrass greens, and the risk of herbicide resistance remains an issue, thus use of plant growth regulators (PGRs) is still the primary method of annual bluegrass suppression. This study was conducted to evaluate eight PGR treatments, employed as a series of 15 consecutive, biweekly applications to suppress annual bluegrass encroachment in ‘Pure Distinction’ creeping bentgrass maintained as a golf course putting green in Los Angeles, CA. Best annual bluegrass suppression was observed with products containing flurprimidol (FP) at 0.256 lb/acre, paclobutrazol (PB) at 0.119 lb/acre, or three-way mixture of FP, trinexapac-ethyl (TE), and PB (FP+PB+TE) at 0.055, 0.014, and 0.055 lb/acre, respectively. Although all treatments caused some significant creeping bentgrass injury, which increased over time, PB at 0.119 lb/acre and FP+PB+TE at 0.055, 0.014, and 0.055 lb/acre, respectively, appeared to be safest among effective treatments. Additionally, those treatments caused significantly darker green turf, which may be desirable on putting greens. This research confirms the potential of PGR use to limit annual bluegrass infestation on creeping bentgrass greens in a Mediterranean climate and reveals the most effective treatments that could be used in a putting green maintenance program.