An integrated process: Ester synthesis in an enzymatic membrane reactor and water sorption

Trusek-Hołownia, A.; Noworyta, A.

doi:10.1016/j.jbiotec.2007.03.006

Cited by 41 publications

(20 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the increase of geraniol concentration, the response geraniol conversion rate almost unchanged, which represented that geraniol had no inhibitory effect on the enzyme esterification activity, proving the high efficiency of the CALB-displaying yeast. This phenomenon had been reported by Trusek-Holownia et al who worked with CALB on geranyl esters synthesis reaction kinetics [16], and some articles about other lipase [17,18].…”

Section: Mutual Effect Of Parameterssupporting

confidence: 57%

Geranyl Butyrate Production by Candida antarctica Lipase B-Displaying Pichia pastoris

Jin

Ntwali

Lin

et al. 2015

Lecture Notes in Electrical Engineering

View full text Add to dashboard Cite

Enzyme-displaying whole-cell yeast is becoming an alternative to immobilized enzyme. Here, a recombinant Pichia pastoris displaying Candida antarctica lipase B (CALB) was used to catalyze the synthesis of geranyl butyrate with geraniol and butanoic acid as substrates in n-heptane. Response surface methodology (RSM) with a four-variable five-level central composite design (CCD) was applied when biocatalyst concentration, geraniol concentration, butanoic acid/ geraniol molar ratio, and water activity were taken as influential factors to improve the production of geranyl butyrate. The results indicated that both substrates molar ratio and water activity affected significantly the geranyl butyrate yield (p < 0.05). Biocatalyst concentration 20.9 g/L, geraniol concentration 0.57 mol/L, butanoic acid/geraniol molar ratio 1.21:1, and water activity 0.53 were the optimum of the ester synthesis. Under the above conditions, the geraniol conversion rate was 98.5 % in 10 mL reaction after 9 h reaction, which was equivalent to 126 g/L geranyl butyrate. When the esterification reaction was scaled up in batch stirred reactors, 97.87 % of geraniol conversion rate in 200 mL as well as 97.56 % in 500 mL could be obtained.

show abstract

Section: Mutual Effect Of Parameterssupporting

confidence: 57%

Geranyl Butyrate Production by Candida antarctica Lipase B-Displaying Pichia pastoris

Jin

Ntwali

Lin

et al. 2015

Lecture Notes in Electrical Engineering

View full text Add to dashboard Cite

show abstract

“…As for lipase-immobilized membrane bioreactors, they are usually used in the field of non-aqueous phase catalysis such as synthesis (74)(75)(76)(77)(78)(79), hydrolysis (80)(81)(82) and kinetic resolution of compounds (83)(84)(85)(86)(87)(88)(89). For example, Tan et al fabricated a lipase-immobilized membrane bioreactor for synthesis of 2-ethylhexyl palmitate, obtaining an average esterification degree of 95% and a product purity of 98% (77).…”

Section: Potential Applications In Bioreactorsmentioning

confidence: 99%

Polymer materials for enzyme immobilization and their application in bioreactors

Fang¹,

Huang²,

Chen³

et al. 2011

BMB Reports

View full text Add to dashboard Cite

Enzymatic catalysis has been pursued extensively in a wide range of important chemical processes for their unparalleled selectivity and mild reaction conditions. However, enzymes are usually costly and easy to inactivate in their free forms. Immobilization is the key to optimizing the in-service performance of an enzyme in industrial processes, particularly in the field of non-aqueous phase catalysis. Since the immobilization process for enzymes will inevitably result in some loss of activity, improving the activity retention of the immobilized enzyme is critical. To some extent, the performance of an immobilized enzyme is mainly governed by the supports used for immobilization, thus it is important to fully understand the properties of supporting materials and immobilization processes. In recent years, there has been growing concern in using polymeric materials as supports for their good mechanical and easily adjustable properties. Furthermore, a great many work has been done in order to improve the activity retention and stabilities of immobilized enzymes. Some introduce a spacer arm onto the support surface to improve the enzyme mobility. The support surface is also modified towards biocompatibility to reduce non-biospecific interactions between the enzyme and support. Besides, natural materials can be used directly as supporting materials owning to their inert and biocompatible properties. This review is focused on recent advances in using polymeric materials as hosts for lipase immobilization by two different methods, surface attachment and encapsulation. Polymeric materials of different forms, such as particles, membranes and nanofibers, are discussed in detail. The prospective applications of immobilized enzymes, especially the enzyme-immobilized membrane bioreactors (EMBR) are also discussed. [BMB reports 2011; 44(2): 87-95]

show abstract

“…Due to the requirements for enzyme activity and microorganism functioning, the reaction phase in biotechnological processes consists of the water phase, except for the systems that involve lipases (Dabrowska et al, 2012;Trusek-Holownia and Noworyta, 2007). The delivery of the product to the organic phase is dependent on separating it from other reactants, including mixtures of enantiomers (Huang et al, 2004;Jin et al, 2003;Ma et al, 2006;Nomoto et al, 2003), shifting the reaction equilibrium to favour the products (Hossain et al, 2008, Vu et al, 2013Wasewar et al, 2004), and preventing the precipitation of a hydrophobic product in the reaction phase after the saturation concentration is exceeded (Jakubke et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic synthesis of hydrophobic compounds integrated with membrane separation

Noworyta

Trusek-Hołownia

2016

Chemical and Process Engineering

View full text Add to dashboard Cite

The enzymatic synthesis of a highly hydrophobic product (dipeptide precursor) in which the reaction is accompanied by the mass transfer of the reaction product to the organic phase and the substrates to the water phase is considered. Equations describing both continuous and batch processes are formulated. The range of variability in the operating parameters of such a bioreactor is specified, and the correlations reported in the literature to describe mass transfer in the membrane contactor are validated. The proposed process was verified experimentally, and good agreement between the determined and calculated concentrations was obtained in both phases.

show abstract

An integrated process: Ester synthesis in an enzymatic membrane reactor and water sorption

Cited by 41 publications

References 38 publications

Geranyl Butyrate Production by Candida antarctica Lipase B-Displaying Pichia pastoris

Geranyl Butyrate Production by Candida antarctica Lipase B-Displaying Pichia pastoris

Polymer materials for enzyme immobilization and their application in bioreactors

Enzymatic synthesis of hydrophobic compounds integrated with membrane separation

Contact Info

Product

Resources

About