An integrated process for the recovery of high added-value compounds from olive oil using solid support free liquid-liquid extraction and chromatography techniques

Assessment of the antioxidant activity of an olive oil total polyphenolic fraction and hydroxytyrosol from a Greek Olea europea variety in endothelial cells and myoblasts

“…EVOO was procured from Northern Greece (Mount Athos) which was freshly produced in January 2015). The extraction of TPF was achieved following a previously described procedure ( 35 ). Liquid-liquid extraction was carried out on a laboratory-scales Fast Centrifugal Partition Extractor FCPE300 ® (Rousselet-Robatel Kromaton, Anonay, France; column capacity of 300 ml) connected to a LabAlliance preparative pump.…”

“…The TPF was fractionated in a step-gradient elution mode following a previously described method ( 35 ). For this purpose, a series of 4 biphasic systems consisting of the solvents, n -hexane, ethyl acetate, etOH, and water in proportions 4/1/2/3 (S1), 3/2/2/3 (S2), 2/3/2/3 (S3) and 1/4/2/3 v/v/v/v (S4) were used.…”

NMR spectroscopy: a powerful tool for the analysis of polyphenols in extra virgin olive oil

“…A new quantitative method involves selective excitation with a double pulsed field gradient ‘perfect echo’ (SELDPFGPE) sequence, consisting of selective experiments with pulses that only excite the region containing the minor analytes of interest, while excluding the spectral regions containing strong lipid signals . An advantage of this method is the ultra‐rapid screening (3 min) of target compounds without any extraction, separation or derivatization . In a recent study, the quantification of OLC and ligstroside aglycone of EVOO samples from all major international brands available in supermarkets in California was achieved by the integration of the doublets at 9.23 and 9.49 ppm respectively, in CDCl 3 at 600 MHz .…”

“…28 This technique has also been used to predict the geographical origin of selected Italian and Greek olive oils 39 and to characterize Italian EVOO. 10,40 A new quantitative method involves selective excitation with a double pulsed field gradient 'perfect echo' (SELDPFGPE) sequence, 41 consisting of selective experiments with pulses that only excite the region containing the minor analytes of interest, while excluding the spectral regions containing strong lipid signals. 28 An advantage of this method is the ultra-rapid screening (3 min) of target compounds without any extraction, separation or derivatization.…”

“…41 In a recent study, the quantification of OLC and ligstroside aglycone of EVOO samples from all major international brands available in supermarkets in California was achieved by the integration of the doublets at 9.23 and 9.49 ppm respectively, in CDCl 3 at 600 MHz. 41 In another study, the enhanced detection of aldehydes in EVOO by means of band-selective NMR spectroscopy allowed the assignment of the two characteristic aldehydes of OLC by extending the DPFGSE technique to the 2D-HSQC experiments, in CDCl 3 at 500 MHz. The first aldehyde at 9.62 ppm was coupled with two aliphatic hydrogen atoms (at 2.97 and 2.73 ppm) and the second was in the region of 9.30-9.15 ppm, with aldehydic resonances showing very strong correlations with a single CH alkene signal (6.60 ppm).…”