An Integrated Quad-Modality Molecular Imaging System for Small Animals

BackgroundThe consumption of large amounts of dietary fats activates an inflammatory response in the hypothalamus, damaging key neurons involved in the regulation of caloric intake and energy expenditure. It is currently unknown why the mediobasal hypothalamus is the main target of diet-induced brain inflammation. We hypothesized that dietary fats can damage the median eminence blood/spinal fluid interface.MethodsSwiss mice were fed on a high-fat diet, and molecular and structural studies were performed employing real-time PCR, immunoblot, immunofluorescence, transmission electron microscopy, and metabolic measurements.ResultsThe consumption of a high fat diet was sufficient to increase the expression of inflammatory cytokines and brain-derived neurotrophic factor in the median eminence, preceding changes in other circumventricular regions. In addition, it led to an early loss of the structural organization of the median eminence β1-tanycytes. This was accompanied by an increase in the hypothalamic expression of brain-derived neurotrophic factor. The immunoneutralization of brain-derived neurotrophic factor worsened diet-induced functional damage of the median eminence blood/spinal fluid interface, increased diet-induced hypothalamic inflammation, and increased body mass gain.ConclusionsThe median eminence/spinal fluid interface is affected at the functional and structural levels early after introduction of a high-fat diet. Brain-derived neurotrophic factor provides an early protection against damage, which is lost upon a persisting consumption of large amounts of dietary fats.Electronic supplementary materialThe online version of this article (10.1186/s12974-017-1046-8) contains supplementary material, which is available to authorized users.

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“…Body composition was obtained by positron emission tomography/CT scan (Albira/Bruker, Billerica, MA, USA) as previously described [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Dietary fats promote functional and structural changes in the median eminence blood/spinal fluid interface—the protective role for BDNF

Ramalho

Bombassaro

Dragano

et al. 2018

J Neuroinflammation

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“…While other groups mainly focus on integrating nuclear imaging with three-dimensional (3D) fluorescence optical tomography (FOT) imaging [ 15 ]-[ 18 ] or fluorescence-mediated tomography (FMT) [ 19 ], we consider optical imaging, both bioluminescence and fluorescence, as intrinsically superficial technology, currently most suitable for planar imaging. Today, planar imaging is applied in the far majority of optical imaging studies in preclinical research since optical tomography is still highly challenging due to the ill-posed nature of optical data caused by strong light absorption and diffusion [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

U-SPECT-BioFluo: an integrated radionuclide, bioluminescence, and fluorescence imaging platform

et al. 2014

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BackgroundIn vivo bioluminescence, fluorescence, and single-photon emission computed tomography (SPECT) imaging provide complementary information about biological processes. However, to date these signatures are evaluated separately on individual preclinical systems. In this paper, we introduce a fully integrated bioluminescence-fluorescence-SPECT platform. Next to an optimization in logistics and image fusion, this integration can help improve understanding of the optical imaging (OI) results.MethodsAn OI module was developed for a preclinical SPECT system (U-SPECT, MILabs, Utrecht, the Netherlands). The applicability of the module for bioluminescence and fluorescence imaging was evaluated in both a phantom and in an in vivo setting using mice implanted with a 4 T1-luc + tumor. A combination of a fluorescent dye and radioactive moiety was used to directly relate the optical images of the module to the SPECT findings. Bioluminescence imaging (BLI) was compared to the localization of the fluorescence signal in the tumors.ResultsBoth the phantom and in vivo mouse studies showed that superficial fluorescence signals could be imaged accurately. The SPECT and bioluminescence images could be used to place the fluorescence findings in perspective, e.g. by showing tracer accumulation in non-target organs such as the liver and kidneys (SPECT) and giving a semi-quantitative read-out for tumor spread (bioluminescence).ConclusionsWe developed a fully integrated multimodal platform that provides complementary registered imaging of bioluminescent, fluorescent, and SPECT signatures in a single scanning session with a single dose of anesthesia. In our view, integration of these modalities helps to improve data interpretation of optical findings in relation to radionuclide images.

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“…57 metastases. 58 The holmium content in our 165 Ho-MS nanoparticles is higher than most reported holmium containing particles. This is an advantage since higher the amount of holmium, lower the dose of material is required for radiotherapy.…”

mentioning

confidence: 52%

Chemoradiotherapeutic wrinkled mesoporous silica nanoparticles for use in cancer therapy

et al. 2014

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Over the last decade, the development and application of nanotechnology in cancer detection, diagnosis, and therapy have been widely reported. Engineering of vehicles for the simultaneous delivery of chemo- and radiotherapeutics increases the effectiveness of the therapy and reduces the dosage of each individual drug required to produce an observable therapeutic response. We here developed a novel chemoradiotherapeutic 1,2-dioleoyl-sn-glycero-3-phosphocholine lipid coated/uncoated platinum drug loaded, holmium-containing, wrinkled mesoporous silica nanoparticle. The materials were characterized with TEM, FTIR, 1H NMR, energy dispersive x-ray, inductively coupled plasma-mass spectrometry, and zeta potential measurements. In vitro platinum drug release from both lipid coated and uncoated chemoradiotherapeutic wrinkled mesoporous silica are reported. Various kinetic models were used to analyze the release kinetics. The radioactivity of the chemoradiotherapeutic nanocarriers was measured after neutron-activation.

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An Integrated Quad-Modality Molecular Imaging System for Small Animals

Cited by 25 publications

References 25 publications

Dietary fats promote functional and structural changes in the median eminence blood/spinal fluid interface—the protective role for BDNF

Dietary fats promote functional and structural changes in the median eminence blood/spinal fluid interface—the protective role for BDNF

U-SPECT-BioFluo: an integrated radionuclide, bioluminescence, and fluorescence imaging platform

Chemoradiotherapeutic wrinkled mesoporous silica nanoparticles for use in cancer therapy

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