2022
DOI: 10.1016/j.scitotenv.2021.149858
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An integrated systems-level model of the toxicity of brevetoxin based on high-resolution magic-angle spinning nuclear magnetic resonance (HRMAS NMR) metabolic profiling of zebrafish embryos

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Cited by 15 publications
(19 citation statements)
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References 136 publications
(182 reference statements)
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“…In actuality, NMR and MS instruments are capable of separating, detecting, and characterizing hundreds to thousands of chemicals in complex chemical mixtures, such as biofluids or tissue extracts [62]. We found four studies that used NMR and three that used MS to study the impact of marine neurotoxins (BTX and BMAA) on the metabolome changes in various toxicological models such as O. melastigma, zebrafish Danio rerio, mouse neuronal cell lines, and rats [63][64][65][66]. Interestingly, these results showed that BTX exposure led to a dysregulation of metabolites involved in neuronal excitotoxicity, whereas BMAA seemed to alter energetic metabolic pathways (Table 3).…”
Section: Metabolomicsmentioning
confidence: 99%
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“…In actuality, NMR and MS instruments are capable of separating, detecting, and characterizing hundreds to thousands of chemicals in complex chemical mixtures, such as biofluids or tissue extracts [62]. We found four studies that used NMR and three that used MS to study the impact of marine neurotoxins (BTX and BMAA) on the metabolome changes in various toxicological models such as O. melastigma, zebrafish Danio rerio, mouse neuronal cell lines, and rats [63][64][65][66]. Interestingly, these results showed that BTX exposure led to a dysregulation of metabolites involved in neuronal excitotoxicity, whereas BMAA seemed to alter energetic metabolic pathways (Table 3).…”
Section: Metabolomicsmentioning
confidence: 99%
“…This leads to NMDA receptor activation, neuronal excitotoxicity, and also the metabolism of proteins involved in NMDA receptors [105]. Second, Annunziato et al [64] used HRMAS-NMR analysis on zebrafish embryos after BTX-2 exposure. Similarly, the authors demonstrated neuronal excitotoxicity in response to the mechanism.…”
Section: In Vivo Studiesmentioning
confidence: 99%
“…A comprehensive system level tracking of the toxicity pathways affected by PET NPs is necessary to understand the toxicity mechanism of PET NPs.Zebrafish (Danio rerio) is used as a model organism in a wide range of developmental toxicological studies [42][43][44][45][46][47] . Performing developmental toxicity studies using the zebrafish embryo model is advantageous for multiple reasons; (1) to generate a robust sample for downstream applications (transcriptomics, proteomics, and metabolomics) a large number of zebrafish embryos can be exposed in small volumes (e.g., ~ 10 embryos per mL); (2) the pore size of the chorion (0.5-0.7 µm) allows to study a wide range of small molecules 48 ; (3) various developmental stages, from fertilized egg to free-swimming larvae can be studied in short time duration (from 1 h up to few days); (4) a growing range of biochemical and molecular technologies including "omics" approaches have been adapted to zebrafish embryos and larvae [42][43][44][45] . Among these, several mass spectrometric (MS) and nuclear magnetic resonance (NMR) spectroscopic tools are available for extracts obtained from zebrafish embryos [49][50][51] .…”
mentioning
confidence: 99%
“…Among these, several mass spectrometric (MS) and nuclear magnetic resonance (NMR) spectroscopic tools are available for extracts obtained from zebrafish embryos [49][50][51] . Compared to other techniques high-resolution magic-angle spinning (HRMAS) NMR is a non-destructive technique used to study metabolic profile in intact tissues or organism [43][44][45][46] . In earlier studies, HRMAS NMR has been successfully utilized to investigate alteration of metabolic profiles by various toxicants in early life stages, i.e., embryos and larvae, of the zebrafish [43][44][45][46] .The aim of the present study is to assess the potential toxicity of PET NPs on zebrafish embryos and to get comprehensive system level understanding of the metabolic pathways affected by PET NPs.…”
mentioning
confidence: 99%
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