An Integrative Pharmacology-Based Approach for Evaluating the Potential Effects of Purslane Seed in Diabetes Mellitus Treatment Using UHPLC-LTQ-Orbitrap and TCMIP V2.0

Abstract

Portulaca oleracea L., known as the “vegetable for long life,” is an annual succulent herb that is widely distributed worldwide. Many clinical and experimental studies have demonstrated that purslane seed (MCXZ) can be used as an adjunctive and alternative therapy for the treatment of diabetes mellitus (DM). However, the underlying active constituents and pharmacological mechanisms through which MCXZ exerts effects in DM remain unclear. In the present study, we confirmed that MCXZ treatment resulted in hypoglycemic activity, lowering the fasting blood glucose and glycated hemoglobin levels in streptozotocin-induced diabetic mice. Then, ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry was used to systematically analyze the chemical profile of MCXZ, resulting in the identification of 84 constituents, including 31 organic acids and nine flavonoids. Finally, the Integrative Pharmacology-based Research Platform of Traditional Chinese Medicine was employed to analyze the key active components of MCXZ and the molecular mechanisms through which these components acted in DM. Ten key active compounds were identified based on the topological importance of their corresponding putative targets within the known DM-associated therapeutic target network of known MCXZ putative targets. Functionally, these candidate targets play critical anti-hyperlipidemia, anti-hyperglycemia, immunity regulation, and inflammatory roles involving DM-related pathways, such as the vascular endothelial growth factor (VEGF) signaling pathway and Fc gamma R-mediated phagocytosis, which indicated that MCXZ exhibited anti-diabetic activity through multi-faced actions.

Identification of the Main Chemical constituents and mechanism of Renshen Guben oral liquid against Renal Fibrosis

“…The potential targets of the compounds identified in RSGB were predicted using the TCM target prediction and functional analysis module of TCMIP v2.0 ( http://www.tcmip.cn/ )[ 12 – 14 ]. The prediction accuracy was set to 0.70 (medium and high similarity).…”

Angong niuhuang wan attenuates LPS-induced acute lung injury by inhibiting PIK3CG/p65/MMP9 signaling in mice based on proteomics

“…The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP, http://tcmspw.com/tcmsp.php ) [ 18 ] was utilized to identify the chemical constituents of 11 Traditional Chinese Medicines screening process for active components in AGNHW relied on an oral drug concentration (OB) ≥ 30% and drug-like drugs (DL) ≥ 0.18, yielding a total of 60 effective chemical components after elimination of duplicates ( Supplementary Table S1 ). Based on the AGNHW component collection results, a research platform grounded on the Integrative Pharmacology-based Research Platform of Traditional Chinese Medicine (TCMIP V2.0, http://www.tcmip.cn/TCMIP/index.php ) [ 19 , 20 ] was implemented. The fundamental principle of target prediction is to utilize MedChem Studio (version 3.0) software to detect the structural similarity between the two-dimensional structure of chemical components in DrugBank and approved certified drugs, and use the Tanimoto coefficient to score their similarities.…”

Nutritional values, bioactive compounds and health benefits of purslane ( Portulaca oleracea L.): a comprehensive review

“…Currently, research about purslane seeds is arising and mostly focus on its potential anti-hyperglycemic effects [117][118][119]. A study in 2021 showed that purslane seeds can lower blood glucose levels, potentially affect phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1), TNF, PPAR-γ, and other targets associated with insulin resistance and inflammation through the actions of various chemical components including genistein, levodopa, and daidzein etc [117].…”