An intensive multilocation temporal dataset of fungal communities in the root and rhizosphere of Brassica napus

Bazghaleh, Navid; Mamet, Steven D.; Bell, Jennifer K.; Moreira, Zayda Piedad Morales; Taye, Zelalem M.; Williams, Shanay; Lamb, Eric G.; Shirtliffe, Steven J.; Vail, Sally; Siciliano, Steven D.; Helgason, Bobbi L.

doi:10.1016/j.dib.2020.105467

Cited by 4 publications

(3 citation statements)

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“…Differences in these traits will result in differences in oil quality and are related to phenological development. Bazghaleh et al (2020) have described the experimental design extensively, but briefly, the site consisted of three replicate blocks with each B. napus line seeded randomly within each block, but not repeated within a block. All lines were planted on May 28, 2017.…”

Section: Methodsmentioning

confidence: 99%

Brassica napus phyllosphere bacterial composition changes with growth stage

2021

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Aims Phyllosphere bacteria play critical roles in plant growth promotion, disease suppression and global nutrient cycling but remain understudied. Methods In this project, we examined the bacterial community on the phyllosphere of eight diverse lines of Brassica napus for ten weeks in Saskatoon, Saskatchewan Canada. Results The bacterial community was shaped largely by plant growth stage with distinct communities present before and after flowering. Bacterial diversity before flowering had 111 core members with high functional potential, with the peak of diversity being reached during flowering. After flowering, bacterial diversity dropped quickly and sharply to 16 members of the core community, suggesting that the plant did not support the same functional potential anymore. B. napus line had little effect on the larger community, but appeared to have more of an effect on the rare bacteria. Conclusions Our work suggests that the dominant bacterial community is driven by plant growth stage, whereas differences in plant line seemed to affect rare bacteria. The role of these rare bacteria in plant health remains unresolved.

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Section: Methodsmentioning

confidence: 99%

Brassica napus phyllosphere bacterial composition changes with growth stage

2021

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“…Sixteen diverse lines of B. napus that represented diverse phenotypes and genotypes were grown at Saskatchewan, Canada in 2016 and 2017 (Table S1). Full details on the data can be found in a previously published data paper (Bazghaleh et al 2020). Brie y, in 2016, sixteen lines were grown at the Agriculture and Agri-Food Canada (AAFC) Llewellyn Research Farm.…”

Section: Experimental Design and Data Collectionmentioning

confidence: 99%

From plant genetics to environment of selection: exploring the drivers of fungal communities in the roots and rhizosphere of Brassica napus

Bazghaleh¹,

Li²,

Vail³

et al. 2022

Preprint

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PurposeOur aim was to characterize the fungal root and rhizosphere microbiomes of genetically diverse Brassica napus lines in a temporally-intensive, multi-site field study to assess the relative contributions of plant genetics, growth stage and environmental conditions to microbiome composition and to identify fungi that were associated with yield performance. MethodsSixteen B. napus lines were grown across three sites in the Canadian Prairies. Sixteen lines were sampled weekly for ten weeks at one site in 2016, as well as a subset of eight lines at the same site in 2017; the sixteen lines were sampled three times at three sites in 2017. The root and rhizosphere fungal microbiomes were assessed using amplicon sequencing of the fungal ITS region with the Illumina MiSeq. ResultsOverall, B. napus line was associated with only 2% of the variation in community structure. Constrained to within a single site-year, this association increased to between 4 and 16% and to between 25 and 37% in a single week within individual sites. The fungal core microbiome consisted of 38 ASVs; Olpidium, Fusicola, Fusarium, Gibberella, Mortierella, and Cutaneotrichosporon were the most abundant taxa with varied abundance during different B. napus growth stages. Thirteen ASVs across three growth stages were highly associated with B. napus yield. ConclusionOur results point to the potential to exploit the B. napus microbiome for improving plant performance by targeting the core taxa identified here as well as those that lead to greater fitness under more specific conditions.

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“…This dataset is a valuable resource that can be used to systematically extract information on the temporal dynamics of microbial communities in the root endosphere and rhizosphere soil of diverse B. napus lines in different soils and across years. The purpose of this report is to provide a publicly available microbiome dataset, its associated metadata and the context by which others explore how bacterial and fungal microbiomes relate to varieties better suited to sustainable crop production, and improve food security worldwide [7] , [8] .…”

Section: Data Descriptionmentioning

confidence: 99%

An intensive multilocation temporal dataset of fungal and bacterial communities in the root and rhizosphere of Brassica napus

et al. 2020

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The plant microbiome has been recently recognized as a plant phenotype to help in the food security of the future population. However, global plant microbiome datasets are insufficient to be used effectively for breeding this new generation of crop plants. We surveyed the diversity and temporal composition of bacterial and fungal communities in the root and rhizosphere of Brassica napus , the world's second largest oilseed crop, weekly in eight diverse lines at one site and every three weeks in sixteen lines, at three sites in 2016 and 2017 in the Canadian Prairies. We sequenced the bacterial 16S ribosomal RNA gene generating a total of 127.7 million reads and the fungal internal transcribed spacer (ITS) region generating 113.4 million reads. 14,944 unique fungal amplicon sequence variants (ASV) were detected, with an average of 43 ASVs per root and 105 ASVs per rhizosphere sample. We detected 10,882 unique bacterial ASVs with an average of 249 ASVs per sample. Temporal, site-to-site, and line-driven variability were key determinants of microbial community structure. This dataset is a valuable resource to systematically extract information on the belowground microbiome of diverse B. napus lines in different environments, at different times in the growing season, in order to adapt effective varieties for sustainable crop production systems.

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An intensive multilocation temporal dataset of fungal communities in the root and rhizosphere of Brassica napus

Cited by 4 publications

References 12 publications

Brassica napus phyllosphere bacterial composition changes with growth stage

Brassica napus phyllosphere bacterial composition changes with growth stage

From plant genetics to environment of selection: exploring the drivers of fungal communities in the roots and rhizosphere of Brassica napus

An intensive multilocation temporal dataset of fungal and bacterial communities in the root and rhizosphere of Brassica napus

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