An International Reference Reagent for the detection of anti‐human neutrophil antigen‐1a

Abstract

In October 2011, the WHO Expert Committee on Biological Standardization approved the material 09/284 as an International Reference Reagent for the detection of anti-HNA-1a.

Evaluation of HNA-expressing cell line-based antigen capture systems and a solid-phase system for detecting HNA-1a antibodies

“…Although the specificity was greater than 0.93 in all the systems, the sensitivity varied among the systems. The sensitivity (0.55) of MAIGA using 3G8 was much lower than that of the MAIGA systems using the other MoAbs (0.91), and only this system failed to detect HNA-1a Abs in the WHO sample, which is consistent with a previous study [21]. Although KY-INA detected the target Ab in the WHO sample, this assay had the lowest sensitivity (0.36) among the 5 systems.…”

“…To compare the KY-MAIGA with the conventional neutrophil-based MAIGA, we examined the maximum dilution in which HNA-1a Ab in the WHO sample could be detected in KY-MAIGA with LNK16, and compared with the maximum dilution reported using neutrophil-based MAIGA with LNK16 [21]. The maximum dilution in KY-MAIGA was 32-fold, while that in the neutrophil-based MAIGA had been reported to be 8-fold, suggesting that KY-MAIGA may be more sensitive than neutrophil-based MAIGA.…”

“…Three groups of serum samples were used as HNA-1a Ab-positive samples: (1) the International Reference Reagent for the detection of HNA-1a, approved by the World Health Organization (WHO) Expert Committee on Biological Standardization in October 2011 [21]; (2) two samples derived from blood products of non-haemolytic transfusion reaction cases and classified as HNA-1a Ab positive by the Committee of Control Survey for HLA Abs and HNA Abs of the Japanese Red Cross Society; both of the samples tested GIFT positive, 5 cell-lineage IFT positive and KY-IFT positive; (3) eight samples from cases of neutropenia tested for HNA Abs in response to requests by the hospitals and classified as HNA-1a Ab positive using the 5 celllineage IFT and KY-IFT, with a criterion in which samples were classified positive when they were positive in either of the two tests. All the eight samples were positive in the KY-IFT, while six were positive in the 5 cell-lineage IFT.…”

Production of recombinant humanized monoclonal anti‐human neutrophil antigen (HNA) antibodies with potential applicability as standard antibodies

“…To the best of our knowledge, the international standard HNA‐1a (NIBSC code 09/284), 21 and anti‐HNA‐3a (preparation 19/114) antibodies are the only publicly available reference reagents for granulocyte serology supplied by the WHO. However, these samples of human origin have limited availability, and new reference reagents with good specificity and without supply restrictions are required.…”

Prevalência, perfil clínico e evolutivo de neutropenias crônicas autoimunes em crianças e adolescentes acompanhados em ambulatórios terciários de hematologia, imunologia e reumatologia

“…A detecção de autoanticorpos que causam NAI é difícil devido a natureza lábil dos granulócitos (Cardoso et al, 2013;Lucas et al, 2013;Metcalfe et al, 2013).…”