An interspecies approach to the investigation of the red cell membrane glucose transporter

Wagner, Reiner; Zimmer, Guido; Lacko, L.

doi:10.1016/0005-2736(84)90115-9

Cited by 24 publications

(9 citation statements)

References 18 publications

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“…Our studies confirmed that the amounts of 18 F-FDG in the whole-blood samples were very different from those in the plasma samples after 18 F-FDG injection (Fig. 4A) (12). These results suggested that the 18 F-FDG activities in the whole-blood samples could not be used reliably as an input function for 18 F-FDG kinetic analysis.…”

Section: Discussionsupporting

confidence: 65%

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In Vivo Quantitation of Glucose Metabolism in Mice Using Small-Animal PET and a Microfluidic Device

Wu¹,

Sui²,

Lee³

et al. 2007

Journal of Nuclear Medicine

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The challenge of sampling blood from small animals has hampered the realization of quantitative small-animal PET. Difficulties associated with the conventional blood-sampling procedure need to be overcome to facilitate the full use of this technique in mice. Methods: We developed an automated blood-sampling device on an integrated microfluidic platform to withdraw small blood samples from mice. We demonstrate the feasibility of performing quantitative small-animal PET studies using 18 F-FDG and input functions derived from the blood samples taken by the new device. 18 F-FDG kinetics in the mouse brain and myocardial tissues were analyzed. Results: The studies showed that small (;220 nL) blood samples can be taken accurately in volume and precisely in time from the mouse without direct user intervention. The total blood loss in the animal was ,0.5% of the body weight, and radiation exposure to the investigators was minimized. Good model fittings to the brain and the myocardial tissue time-activity curves were obtained when the input functions were derived from the 18 serial blood samples. The R 2 values of the curve fittings are .0.90 using a 18 F-FDG 3-compartment model and .0.99 for Patlak analysis. The 18 F-FDG rate constants K, and k Ã 4 , obtained for the 4 mouse brains, were comparable. The cerebral glucose metabolic rates obtained from 4 normoglycemic mice were 21.5 6 4.3 mmol/min/100 g (mean 6 SD) under the influence of 1.5% isoflurane. By generating the whole-body parametric images of K Ã FDG (mL/min/g), the uptake constant of 18 F-FDG, we obtained similar pixel values as those obtained from the conventional regional analysis using tissue time-activity curves. Conclusion: With an automated microfluidic blood-sampling device, our studies showed that quantitative small-animal PET can be performed in mice routinely, reliably, and safely in a small-animal PET facility.

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Section: Discussionsupporting

confidence: 65%

“…In addition, we performed a set of mouse studies to obtain the 18 F-FDG ratio curve of the whole blood to plasma. This procedure was necessary because of the relatively slow transport of 18 F-FDG across the membrane of the red blood cells in mice (12,13). We performed 18 F-FDG kinetic analysis using the 3-compartment 18 F-FDG model and the Patlak analysis.…”

mentioning

confidence: 99%

In Vivo Quantitation of Glucose Metabolism in Mice Using Small-Animal PET and a Microfluidic Device

Wu¹,

Sui²,

Lee³

et al. 2007

Journal of Nuclear Medicine

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“…However, for accurate quantification of MGU, it is critical that plasma glucose time-activity curves be used rather than wholeblood time-activity curves. As discussed previously in this journal (2,3), whereas glucose equilibrates extremely rapidly across the erythrocyte plasma membrane in primates, this is not true in adult nonprimates (4,5). Transport of glucose into human erythrocytes was too fast to measure at 37°C, whereas in rat erythrocytes transport was more than 3 orders of magnitude slower, even when compared with human erythrocytes at 4°C (5).…”

mentioning

confidence: 71%

“…As discussed previously in this journal (2,3), whereas glucose equilibrates extremely rapidly across the erythrocyte plasma membrane in primates, this is not true in adult nonprimates (4,5). Transport of glucose into human erythrocytes was too fast to measure at 37°C, whereas in rat erythrocytes transport was more than 3 orders of magnitude slower, even when compared with human erythrocytes at 4°C (5). Slower glucose transport rates result in lower erythrocyte-to-plasma glucose distribution ratios in nonhuman primates; ratios ranged from 0 in pigs to 0.45 in calves (4).…”

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confidence: 80%

Noninvasive Measurement of Mouse Myocardial Glucose Uptake with ¹⁸F-FDG

Buxton

2014

J Nucl Med

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“…Although image-derived input function appears to be an attractive alternative to arterial sampling, imagederived input function is reliable only in selected situations and selected tracers (2). In the case of 18 F-FDG quantitative smallanimal PET, one of the major concerns was the different 18 F-FDG concentrations in plasma and in whole blood (WB) due to a slow erythrocyte 18 F-FDG transport rate in rodent blood (3,4). Therefore, arterial plasma samples remain the gold standard for input function and CMR glc quantification in rats (5).…”

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confidence: 99%

Performing Repeated Quantitative Small-Animal PET with an Arterial Input Function Is Routinely Feasible in Rats

Huang

et al. 2016

J Nucl Med

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Performing quantitative small-animal PET with an arterial input function has been considered technically challenging. Here, we introduce a catheterization procedure that keeps a rat physiologically stable for 1.5 mo. We demonstrated the feasibility of quantitative small-animal 18 F-FDG PET in rats by performing it repeatedly to monitor the time course of variations in the cerebral metabolic rate of glucose (CMR glc ). Methods: Aseptic surgery was performed on 2 rats. Each rat underwent catheterization of the right femoral artery and left femoral vein. The catheters were sealed with microinjection ports and then implanted subcutaneously. Over the next 3 wk, each rat underwent 18 F-FDG quantitative small-animal PET 6 times. The CMR glc of each brain region was calculated using a 3-compartment model and an operational equation that included a k* 4 . Results: On 6 mornings, we completed 12 18 F-FDG quantitative small-animal PET studies on 2 rats. The rats grew steadily before and after the 6 quantitative small-animal PET studies. The CMR glc of the conscious brain (e.g., right parietal region, 99.6 6 10.2 mmol/100 g/min; n 5 6) was comparable to that for 14 C-deoxyglucose autoradiographic methods. Conclusion: Maintaining good blood patency in catheterized rats is not difficult. Longitudinal quantitative small-animal PET imaging with an arterial input function can be performed routinely. Sever al small-animal models have been developed that simulate many of the important hallmarks of human brain diseases. Although the first report of quantitative small-animal PET to monitor the CMR glc changes in a rat model of brain injury appeared in 2000 (1), there has been rare use of quantitative small-animal PET with experimental models over the intervening years. Cannulation of a rat is not difficult. The challenges associated with repeated surgeries to obtain arterial blood samples have most likely impeded routine use of the method. Although image-derived input function appears to be an attractive alternative to arterial sampling, imagederived input function is reliable only in selected situations and selected tracers (2). In the case of 18 F-FDG quantitative smallanimal PET, one of the major concerns was the different 18 F-FDG concentrations in plasma and in whole blood (WB) due to a slow erythrocyte 18 F-FDG transport rate in rodent blood (3,4). Therefore, arterial plasma samples remain the gold standard for input function and CMR glc quantification in rats (5).To ease blood sampling and reduce blood loss in small animals, we developed an automated microfluidic device that allows us to take discrete, serial blood samples (,1 mL per sample) with little human effort (6). However, blood coagulation took place frequently in the catheter when the interval between 2 serial blood samples was long (e.g., .10 min). In this work, we developed a surgical procedure that eases radiotracer injection and arterial blood sampling. We conducted a proof-of-principle study to demonstrate the feasibility of performing multiple-time-point quanti...

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An interspecies approach to the investigation of the red cell membrane glucose transporter

Cited by 24 publications

References 18 publications

In Vivo Quantitation of Glucose Metabolism in Mice Using Small-Animal PET and a Microfluidic Device

In Vivo Quantitation of Glucose Metabolism in Mice Using Small-Animal PET and a Microfluidic Device

Noninvasive Measurement of Mouse Myocardial Glucose Uptake with ¹⁸F-FDG

Performing Repeated Quantitative Small-Animal PET with an Arterial Input Function Is Routinely Feasible in Rats

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An interspecies approach to the investigation of the red cell membrane glucose transporter

Cited by 24 publications

References 18 publications

In Vivo Quantitation of Glucose Metabolism in Mice Using Small-Animal PET and a Microfluidic Device

In Vivo Quantitation of Glucose Metabolism in Mice Using Small-Animal PET and a Microfluidic Device

Noninvasive Measurement of Mouse Myocardial Glucose Uptake with 18F-FDG

Performing Repeated Quantitative Small-Animal PET with an Arterial Input Function Is Routinely Feasible in Rats

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Product

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Noninvasive Measurement of Mouse Myocardial Glucose Uptake with ¹⁸F-FDG