An intramolecular catalytic hairpin assembly on a DNA tetrahedron for mRNA imaging in living cells: improving reaction kinetics and signal stability

Abstract

An intramolecular catalytic hairpin assembly is implemented on a DNA tetrahedron for mRNA imaging in living cells. The spatial confinement effect enables the acceleration of target-triggered signal generation, with excellent cell permeability and FRET signal stability.

Triggered Dimerization and Trimerization of DNA Tetrahedra for Multiplexed miRNA Detection and Imaging of Cancer Cells

“…[ 40–42 ] The unique features of DNA tetrahedra include their structural rigidity, the ability to control their sizes, [ 43,44 ] and the feasibility to dictate the spatial tethering of nucleic acids to the edges or corners of the tetrahedra. [ 45–47 ] Different applications of DNA tetrahedra were reported, including their use as functional units for sensing, [ 48,49 ] logic gate operations, [ 50 ] intracellular imaging, [ 51–54 ] delivery of therapeutic agents, [ 55–57 ] and the assembly of chiral building blocks for nanofabrication and chiroplasmonic. [ 58 ]…”

An Activatable Nanoenzyme Reactor for Coenhanced Chemodynamic and Starving Therapy Against Tumor Hypoxia and Antioxidant Defense System

“…GSH is depleted by MDN to form manganese ion (Mn 2+ ) and glutathione disulfide (GSSG), 7,26,29 in which the FRs are released with a corresponding fluorescence recovery, thus, enabling the detection of the activatable NER. 30,31 26 and its sheet structure was verified by TEM (Figure 1c, left). It was also characterized by UV-Vis absorption and energy dispersive spectrometer (EDS) spectrum (Supporting Information Figure S1); its size and zeta potential were about 125 nm (Supporting Information Figure S2, black curve) and −27.9 mV (Figure 1d, black curve), as measured by DLS.…”

Pt–S Bond‐Mediated Nanoflares for High‐Fidelity Intracellular Applications by Avoiding Thiol Cleavage

“…The AuNP@Pt‐S nanoflare was then used to detect the overexpressed miRNA‐21 in cancer cells . Selective lighting up of the nanoflare in two overexpressed cancer cells was achieved by miRNA‐21‐triggered imaging (Supporting Information, Figure S26).…”