An intraoperative study with <i>ex vivo</i> fluorescence confocal microscopy: diagnostic accuracy of the three visualization modalities

Reggiani, Camilla; Pellacani, Giovanni; Bonetti, Luca Reggiani; Zanelli, G.; Azzoni, Paola; Chester, Johanna; Kaleci, Shaniko; Ferrari, Béatrice; Bellini, Pierantonio; Longo, Caterina; Bertoni, Laura; Magnoni, Cristina

doi:10.1111/jdv.16831

Cited by 8 publications

(4 citation statements)

References 10 publications

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“…Schüürmann et al have recently described in detail the appearance of the different cytological structures of healthy skin in the DS mode [26]. A present‐day publication by Reggiani et al has even already statistically proved the superiority of DS over the other diagnostic modalities for the evaluation of BCCs by both dermatologists and pathologists by reaching impressive 94% overall correct diagnoses with a sensitivity of 83.3% and a specificity of 96.1% [33]. Although DS alone is a breakthrough in ex vivo CLSM, we strongly recommend further research on new staining methods to further increase the diagnostic accuracy of ex vivo CLSM and extend the spectrum of indications.…”

Section: Discussionmentioning

confidence: 99%

Ex vivo confocal laser scanning microscopy: A diagnostic technique for easy real‐time evaluation of benign and malignant skin tumours

Vladimirova

Ruini

Kapp

et al. 2022

Journal of Biophotonics

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Ex vivo confocal laser scanning microscopy (ex vivo CLSM) is a novel diagnostic tool for a quick bedside evaluation of freshly excised tissue, comparable to histology. We aimed to assess the sensitivity and specificity of ex vivo CLSM in detecting malignant features, to validate its reliability in identifying various skin tumours based on a combination of confocal features and to evaluate the digital staining mode (DS). One‐hundred twenty freshly excised skin samples from 91 patients were evaluated. Each lesion was screened for the presence of 23 predefined confocal criteria with ex vivo CLSM, followed by a histopathological examination. The diagnostic agreement between ex vivo CLSM and histology was 89.2%. The diagnostic accuracy of ex vivo CLSM in detecting malignancy reached a sensitivity of 98% and a specificity of 76%. Ex vivo CLSM enabled a rapid identification of the most common skin tumours, the tumour dignity and cytological features. The DS demonstrated a close resemblance to conventional histopathology.

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Section: Discussionmentioning

confidence: 99%

Ex vivo confocal laser scanning microscopy: A diagnostic technique for easy real‐time evaluation of benign and malignant skin tumours

Vladimirova

Ruini

Kapp

et al. 2022

Journal of Biophotonics

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“…Vivascope has previously been used in other areas of medical diagnosis, with high sensitivity and specificity in detecting neoplastic cells [ 16 ]. Its usefulness has been evaluated in diagnosing non-melanoma skin tumors and on other types of surgical samples, including liver biopsy and prostatic tissue, showing how this technique can distinguish between neoplastic and non-neoplastic tissues [ 17 , 18 , 19 ]. The Vivascope 2500M-G4 offers several benefits.…”

Section: Discussionmentioning

confidence: 99%

Potential Use of Vivascope for Real-Time Histological Evaluation in Endoscopic Laryngeal Surgery

Benedetto

Moffa

Baptista

et al. 2023

JPM

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We aimed to assess the feasibility of using confocal laser scanning microscopy (CLSM) for the real-time ex vivo examination of histological samples of laryngeal lesions and to evaluate the correlation between CLSM and definitive histological results. This preliminary study included eight consecutive patients with “suspected” laryngeal lesions who were candidates for endoscopic laryngeal surgery. The obtained samples were evaluated using CLSM and classified as “inadequate” or “adequate” (high- and low-grade dysplasia, in situ and invasive carcinoma, positive surgical margin, and inflammatory outbreaks). CLSM showed the macro image in all cases and generated a digital version. All the samples were defined as adequate during CLSM and confirmed at histopathology: low-grade dysplasia (n = 5), low- and high-grade dysplasia (n = 2), and high-grade dysplasia (n = 1). Four samples had an involved resection margin, and three samples revealed the presence of inflammatory outbreaks. CLSM can be applied to larynx pathology with excellent agreement with final histological results.

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“… 8 FCM is rapidly emerging for real-time microscopic assessment of resection margins in various surgical fields including for skin tumors, urological neoplasms, and prostate cancer 9 , 10 ; and for immediate diagnosis in different organs such as colon, prostate, and thyroid. 11 , 12 , 13 , 14 FCM appears particularly appealing for rapid bedside evaluation of small specimens, such as image-guided fine-needle aspiration biopsy, core needle biopsy, and endoscopic biopsy. 15 , 16 Recently, a method to subject pancreatic EUS-FNA/FNB samples to FCM evaluation was reported based on a polymeric scaffold (Cytomatrix, UCS Diagnostic, Morlupo, Italy) as a holder to keep specimens under the FCM objective and maintain cellular material and fragments during subsequent FFPE preparation.…”

Section: Introductionmentioning

confidence: 99%

New digital confocal laser microscopy may boost real-time evaluation of endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) from solid pancreatic lesions: Data from an international multicenter study

Amendoeira¹,

Arcidiacono²,

Barizzi³

et al. 2022

eBioMedicine

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An intraoperative study with ex vivo fluorescence confocal microscopy: diagnostic accuracy of the three visualization modalities

Cited by 8 publications

References 10 publications

Ex vivo confocal laser scanning microscopy: A diagnostic technique for easy real‐time evaluation of benign and malignant skin tumours

Ex vivo confocal laser scanning microscopy: A diagnostic technique for easy real‐time evaluation of benign and malignant skin tumours

Potential Use of Vivascope for Real-Time Histological Evaluation in Endoscopic Laryngeal Surgery

New digital confocal laser microscopy may boost real-time evaluation of endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) from solid pancreatic lesions: Data from an international multicenter study

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