An Intravital Model to Monitor Steps of Metastatic Tumor Cell Adhesion Within the Hepatic Microcirculation

Haier, Jörg; Korb, Timo; Hotz, Birgit; Spiegel, Hans‐Ullrich; Senninger, N.

doi:10.1016/s1091-255x(03)00023-4

Cited by 74 publications

(106 citation statements)

References 27 publications

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“…Previously, we have shown that the route of cell application (left heart, right heart, portal vein) did not influence the adhesive or migratory behavior within the liver sinusoids. 18 This technique did not interfere with cardiocirculatory or pulmonary functions of the animals. In case of the lung microcirculation, injection time was 120 seconds.…”

Section: In Vivo Observation Of Metastatic Tumor Cell Adhesionmentioning

confidence: 92%

“…These specific adhesive interactions were independent from the route of cell application, and tumor cells showed similar behavior within the liver sinusoids, if they were injected intravenously, intra-arterially, or intraportally. 18 Furthermore, using this model we previously showed that specific cell adhesions mediated by different integrins and selectin ligands at the tumor cell surfaces appear to be required for successful tumor cell arrest within the hepatic microcirculation, 19,20 with tumor cell adhesion modulated by the integrity of cytoskeletal components. 21 This observation of specific adhesive interactions within the hepatic microcirculation suggested an involvement of this step in the organ-specificity of metastasis formation.…”

mentioning

confidence: 97%

“…[15][16][17] Intravital microscopy technologies have been recently used to investigate metastatic tumor cell adhesion within host organ microcirculation, such as in liver and lung. 10 -14 In these studies contradictory results were reported regarding the type of entrapment (mechanical entrapment 10 -12 versus active cell adhesion 14,18 ) and the requirement of invasion into host organ parenchyma (invasion 10,11,13,18 versus intravascular proliferation 14 ). Using colon carcinoma cells, we recently reported a lack of mechanical entrapment in rat liver sinusoids and a rapid extravasation into the liver parenchyma.…”

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confidence: 99%

See 2 more Smart Citations

Organ-Specific Metastatic Tumor Cell Adhesion and Extravasation of Colon Carcinoma Cells with Different Metastatic Potential

Schlüter

Gaßmann

Enns

et al. 2006

The American Journal of Pathology

127

112

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Adhesive and invasive characteristics appear to be crucial for organ-specific metastasis formation. Using intravital microscopy we investigated the relation between the metastatic potential of colon carcinoma cells and their adhesive and invasive behavior during early steps of metastasis within microvasculatures of rat liver, lung, intestine, skin, muscle, spleen, and kidney in vivo. Colorectal carcinomas will affect ϳ6% of the population in the Western countries during their lifetime. These carcinomas are the third leading cause of cancer-related deaths among women and men and are the most important malignancies of the gut.1 Approximately 50% of the cancer patients die within 5 years because of cancerrelated problems, mostly attributable to metastatic lesions caused by the spread of cancers to near and distant sites. Even after potentially curative surgery, more than 30% of the patients with colorectal carcinomas subsequently develop metastases demonstrating that the spread from primary tumors to distant organs is usually the life-limiting aspect in colorectal carcinoma patients. 2Similar to other cancer entities, colorectal carcinomas often show organ preference of metastasis formation. The liver is the most common organ in which colorectal carcinomas establish distant metastases, and the liver is involved in more than 70% of patients with colorectal metastases. However, in 40 to 50% of these patients with liver metastases, other organs are also involved in metastatic colonization. The second most important organ for colorectal cancer metastasis is the lung, and 20 to 30% of all distant colorectal carcinoma metastases are found primarily in the lung. Isolated lung metastases with no evidence of other organ involvement are found in 5 to 10% of colorectal cancer patients. Other organs, such as the central nervous system, adrenal glands, spleen, skeleton, or skin together account for less than 10% of all colorectal metastases. 2,3The metastatic process consists of a number of sequential, interrelated steps that can all be rate limiting. 4,5 An important and early step during formation of distant metastasis appears to be the arrest of circulating tumor cells within the host organ.6,7 Approximately a century ago, two major hypotheses on the mechanisms of tumor cell arrest in metastatic host organs were proposed. Ewing 8 assumed that the random mechanical lodgment of Supported by the Innovative Medizinische Forschung Fund (University Hospital Mü nster) (Ha 1 2 01 01 to J.H.).K.S. and P.G. contributed equally to this study.

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Section: In Vivo Observation Of Metastatic Tumor Cell Adhesionmentioning

confidence: 92%

mentioning

confidence: 97%

mentioning

confidence: 99%

See 1 more Smart Citation

Organ-Specific Metastatic Tumor Cell Adhesion and Extravasation of Colon Carcinoma Cells with Different Metastatic Potential

Schlüter

Gaßmann

Enns

et al. 2006

The American Journal of Pathology

127

112

View full text Add to dashboard Cite

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“…Microcirculation has been successfully studied using optical microscopy in various animal models (e.g., rabbit or mouse ear, hamster or mouse dorsal skin-flap window or skin-fold chamber, or open cremaster muscle) [52][53][54][55]59,63,[70][71][72][73] . The use of these models for high-resolution imaging of individual flowing or static cells may be somewhat limited because of significant light scattering from surrounding tissue (e.g., skin or muscles) and/or the relatively deep location of vessels below the skin.…”

Section: Animal Modelsmentioning

confidence: 99%

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Galanzha¹

2007

WJG

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Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo . Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including in vivo label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cell's functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo-and radio-sensitivity tests, and drug screening, are also discussed. TOPIC HIGHLIGHT

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“…In fact, 15-25% of CRC patients present with synchronous hepatic metastases at the time of diagnosis, and a further 30% will later develop liver metastasis (4,5). The complex network of vessels and microcapillaries of the hepatic microcirculation makes the liver a target for circulating cells (6). Indeed, cancer cells released from a primary lesion follow a natural blood flow directly to the liver, through the specialized microvessel network known as the liver sinusoids.…”

Section: Introductionmentioning

confidence: 99%

Role of liver ICAM-1 in metastasis

2017

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Abstract. Intercellular adhesion molecule (ICAM)-1, is a transmembrane glycoprotein of the immunoglobulin (Ig)-like superfamily, consisting of five extracellular Ig-like domains, a transmembrane domain and a short cytoplasmic tail. ICAM-1 is expressed in various cell types, including endothelial cells and leukocytes, and is involved in several physiological processes. Furthermore, it has additionally been reported to be expressed in various cancer cells, including melanoma, colorectal cancer and lymphoma. The majority of studies to date have focused on the expression of the ICAM-1 on the surface of tumor cells, without research into ICAM-1 expression at sites of metastasis. Cancer cells frequently metastasize to the liver, due to its unique physiology and specialized liver sinusoid capillary network. Liver sinusoidal endothelial cells constitutively express ICAM-1, which is upregulated under inflammatory conditions. Furthermore, liver ICAM-1 may be important during the development of liver metastasis. Therefore, it is necessary to improve the understanding of the mechanisms mediated by this adhesion molecule in order to develop host-directed anticancer therapies.

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An Intravital Model to Monitor Steps of Metastatic Tumor Cell Adhesion Within the Hepatic Microcirculation

Cited by 74 publications

References 27 publications

Organ-Specific Metastatic Tumor Cell Adhesion and Extravasation of Colon Carcinoma Cells with Different Metastatic Potential

Organ-Specific Metastatic Tumor Cell Adhesion and Extravasation of Colon Carcinoma Cells with Different Metastatic Potential

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Role of liver ICAM-1 in metastasis

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