An Introduction To Tissue‐Biomaterial Interactions

Dee, Kay C; Puleo, David A.; Bizios, Rena

doi:10.1002/0471270598

Cited by 339 publications

(381 citation statements)

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“…Furthermore, the proper orientation of the peptide may result in enhanced activity [34]. Towards this goal, the effect of AMP chemical immobilization on antimicrobial activity has been studied by several investigators [3,4,6,[25][26][27]29,48,49,71] (Table 1). In the current review, different AMP surface covalent immobilization strategies are discussed, focusing on the importance of solid supports and chemical coupling strategies, spacer specificities (type, length and flexibility), surface density and exposure/orientation as determinants of immobilized peptide biocidal and cytotoxic activity.…”

Section: Covalent Immobilization Of An Antimicrobial Peptidementioning

confidence: 99%

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Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

et al. 2011

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a b s t r a c tBacterial adhesion to biomaterials remains a major problem in the medical devices field. Antimicrobial peptides (AMPs) are well-known components of the innate immune system that can be applied to overcome biofilm-associated infections. Their relevance has been increasing as a practical alternative to conventional antibiotics, which are declining in effectiveness. The recent interest focused on these peptides can be explained by a group of special features, including a wide spectrum of activity, high efficacy at very low concentrations, target specificity, anti-endotoxin activity, synergistic action with classical antibiotics, and low propensity for developing resistance. Therefore, the development of an antimicrobial coating with such properties would be worthwhile. The immobilization of AMPs onto a biomaterial surface has further advantages as it also helps to circumvent AMPs' potential limitations, such as short half-life and cytotoxicity associated with higher concentrations of soluble peptides. The studies discussed in the current review report on the impact of covalent immobilization of AMPs onto surfaces through different chemical coupling strategies, length of spacers, and peptide orientation and concentration. The overall results suggest that immobilized AMPs may be effective in the prevention of biofilm formation by reduction of microorganism survival post-contact with the coated biomaterial. Minimal cytotoxicity and longterm stability profiles were obtained by optimizing immobilization parameters, indicating a promising potential for the use of immobilized AMPs in clinical applications. On the other hand, the effects of tethering on mechanisms of action of AMPs have not yet been fully elucidated. Therefore, further studies are recommended to explore the real potential of immobilized AMPs in health applications as antimicrobial coatings of medical devices.

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Section: Covalent Immobilization Of An Antimicrobial Peptidementioning

confidence: 99%

“…non-sterile implantation procedures. Finally, deep late infections appear months or years after surgery and may be a delayed display of contamination that was seeded during surgery or resulted from bacteria that migrated from another anatomic site [6].…”

Section: Introductionmentioning

confidence: 99%

Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

et al. 2011

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“…Another graft option is synthetics, like metals and ceramics, which have been used primarily in hip implants [3]. Their limitations are that they do not provide optimal mechanical properties, they exhibit poor overall osseointegration [3], and they eventually fail due to infection or fatigue loading [4,5]. Together these findings underscore the major clinical need for new bone grafting materials.…”

Section: Introductionmentioning

confidence: 99%

Microporous bacterial cellulose as a potential scaffold for bone regeneration

et al. 2010

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a b s t r a c tNanoporous cellulose biosynthesized by bacteria is an attractive biomaterial scaffold for tissue engineering due to its biocompatibility and good mechanical properties. However, for bone applications a microscopic pore structure is needed to facilitate osteoblast ingrowth and formation of a mineralized tissue. Therefore, in this study microporous bacterial cellulose (BC) scaffolds were prepared by incorporating 300-500 lm paraffin wax microspheres into the fermentation process. The paraffin wax microspheres were subsequently removed, and scanning electron microscopy confirmed a microporous surface of the scaffolds while Fourier transform infrared spectroscopy verified the elimination of paraffin and tensile measurements showed a Young's modulus of approximately 1.6 MPa. Microporous BC and nanoporous (control) BC scaffolds were seeded with MC3T3-E1 osteoprogenitor cells, and examined by confocal microscopy and histology for cell distribution and mineral deposition. Cells clustered within the pores of microporous BC, and formed denser mineral deposits than cells grown on control BC surfaces. This work shows that microporous BC is a promising biomaterial for bone tissue engineering applications.

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“…Both mass transfer limitations and fibrosis associated with the foreign body response can contribute to implant necrosis. [9] Although certain features of the foreign body response to the implanted material could lead capillary ingrowth over time, this native angiogenic process is likely to be either too slow or result in a capillary density that is too low to provide sufficient nutrient transport for most cells in constructs approximately one mm thick and greater. [10][11][12] Stimulating the rapid formation of high density local vasculature is thus a topic of great interest in the tissue engineering community.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

Guan

Stankus

Wagner

2007

Journal of Controlled Release

149

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Scaffolds that better approximate the mechanical properties of cardiovascular and other soft tissues might provide a more appropriate mechanical environment for tissue development or healing in vivo. An ability to induce local angiogenesis by controlled release of an angiogenic factor, such as basic fibroblast growth factor (bFGF), from a biodegradable scaffold with mechanical properties more closely approximating soft tissue could find application in a variety of settings. Toward this end biodegradable poly(ester urethane)urea (PEUU) scaffolds loaded with bFGF were fabricated by thermally induced phase separation. Scaffold morphology, mechanical properties, release kinetics, hydrolytic degradation and bioactivity of the released bFGF were assessed. The scaffolds had interconnected pores with porosities of 90% or greater and pore sizes ranging from 34-173 μm. Scaffolds had tensile strengths of 0.25-2.8 MPa and elongations at break of 81-443%. Incorporation of heparin into the scaffold increased the initial burst release of bFGF, while the initial bFGF loading content did not change release kinetics significantly. The released bFGF remained bioactive over 21 days as assessed by smooth muscle mitogenicity. Scaffolds loaded with bFGF showed slightly higher degradation rates than unloaded control scaffolds. Smooth muscle cells seeded into the scaffolds with bFGF showed higher cell densities than for control scaffolds after 7 days of culture. The bFGFreleasing PEUU scaffolds thus exhibited a combination of mechanical properties and bioactivity that might be attractive for use in cardiovascular and other soft tissue applications.

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An Introduction To Tissue‐Biomaterial Interactions

Cited by 339 publications

References 0 publications

Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

Microporous bacterial cellulose as a potential scaffold for bone regeneration

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

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