This study presents the first record of Enoplognatha mandibularis (Lucas, 1846) to the autonomous community of Galicia, Spain. Two adult males were studied, one was accidentally captured in a mosquitoes ovitrap in the municipality of Redondela, Pontevedra province, and the second was found dead inside the Faculty of Biology at the University Campus As Lagoas-Marcosende, Pontevedra province. These findings contribute to the understanding of the distribution of E. mandibularis in the Iberian Peninsula.