An inverted repeat preceding the <i>Bacillus subtilis glpD</i> gene is a conditional terminator of transcription

Holmberg, Christina; Rutberg, Lars

doi:10.1111/j.1365-2958.1992.tb01752.x

Cited by 26 publications

(23 citation statements)

References 20 publications

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“…5). Most likely the inverted repeat is an important control element for the expression of glpF and glpK as has been demonstrated previously for glpD (Holmberg & Rutberg, 1991, 1992.…”

Section: Tc-mentioning

confidence: 86%

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The glpP and glpF genes of the glycerol regulon in Bacillus subtilis

Beijer

Nilsson

Holmberg

et al. 1993

Journal of General Microbiology

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The Bacillus subtilis glpPFKD region contains genes essential for growth on glycerol or glycerol 3-phosphate (G3P). The nucleotide sequence of glpP encoding a regulatory protein and the previously unidentified glpF encoding the glycerol uptake facilitator was determined. glpF is located immediately upstream of glpK and the two genes were shown to constitute one operon which is transcribed separately from glpP. A aA-type promoter and the transcriptional start point for glpFK were identified. In the 5' untranslated leader sequence (UTL) of glpFK mRNA a conserved inverted repeat is found. The repeat is believed to be involved in the control of expression of glpFK by termination/antitermination of transcription, a control mechanism previously suggested for the regulation of glpD encoding G3P dehydrogenase. Expression of glpFK and glpD requires the inducer G3P and the glpP gene product. A 2.9 kb B. subtilis chromosomal DNA fragment containing the glpP open reading frame was cloned to give plasmid pLUM7. pLUM7 contains a functional glpP gene as shown by its ability to complement various gZpP mutants. Immediately upstream of glpP an open reading ffame is found (Owl). Disrupting ORFl by plasmid integration in the B. subtilis chromosome does not affect the ability to grow on glycerol as sole carbon and energy source. With the present report all B. subtilis glp genes located at 75" on the chromosomal map have been identified.

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“…5). Most likely the inverted repeat is an important control element for the expression of glpF and glpK as has been demonstrated previously for glpD (Holmberg & Rutberg, 1991, 1992.…”

Section: Tc-mentioning

confidence: 86%

“…Production of a full length gZpD mRNA is thought to be controlled by termination/antitermination of a transcript initiated at the constitutive gZpD promoter. In the presence of G3P, the gZpP gene product is thought to enhance transcription across and beyond the inverted repeat (Holmberg & Rutberg, 1991, 1992.…”

Section: Introductionmentioning

confidence: 99%

The glpP and glpF genes of the glycerol regulon in Bacillus subtilis

Beijer

Nilsson

Holmberg

et al. 1993

Journal of General Microbiology

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“…aprEconst substitutes the aprE promoter with the glpD promoter (k40 to k1) (Holmberg & Rutberg, 1992). After cleavage with BamHI, the amplified fragment was ligated to BamHI-cleaved pMD432.…”

Section: Construction Of Strainsmentioning

confidence: 99%

“…From pLUS5a, the region containing the transcribed amyE part (j1 to j134) was amplified with PCR using primers amyEconst and amyEBam2. amyEconst substitutes the amyE promoter with the glpD promoter (k40 to k1) (Holmberg & Rutberg, 1992). After cleavage with BamHI, the amplified fragment was ligated to BamHI-cleaved pMD433.…”

Section: Construction Of Strainsmentioning

confidence: 99%

The aprE leader is a determinant of extreme mRNA stability in Bacillus subtilis

2000

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The Bacillus subtilis aprE gene encodes subtilisin, an extracellular proteolytic enzyme produced in stationary phase. The authors examined the stability of aprE mRNA and aprE leader-lacZ fusion mRNA. Both mRNAs were found to be unusually stable, with half-lives longer than 25 min, demonstrating that the aprE leader contains a determinant for extreme mRNA stability. The half-lives were the same in growing and stationary-phase cells. This contrasts with the findings of O. Resnekov et al. (1990) [Proc Natl Acad Sci U S A 87, 8355-8359], which suggested a growth-phase-dependent mechanism for decay of aprE mRNA. The discrepancy is explained by the techniques used. Substitution of two bases or deletion of 25 nucleotides in the aprE leader led to a major difference in its predicted secondary structure and resulted in a fivefold reduction of the half-life of aprE mRNA. The authors also determined the halflife of amyE mRNA, which encodes α-amylase, another stationary-phase, excreted enzyme and found it to be around 5 min. This shows that extreme stability is not a general property of stationary-phase mRNAs encoding excreted enzymes.

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“…glpT is located at 15' on the chromosomal map and is part of an operon that also contains theglpQ gene which encodes a glycerophosphoryl diester phosphodiesterase (Lindgren, 1978 ;Nilsson e t al., 1994). The main control of the glp regulon of B. subtih appears to be by termination/antitermination of transcription (Holmberg & Rutberg, 1991, 1992. In the presence of G3P, the GlpP protein is thought to act as an antiterminator.…”

Section: Introductionmentioning

confidence: 99%

Mutations in the glycerol kinase gene restore the ability of a ptsGHI mutant of Bacillus subtilis to grow on glycerol

et al. 1995

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Although glycerol is not taken up via the phosphotransferase system (PTS) in Bacillus subtilis, some mutations that affect the general components of the PTS impair the ability of cells to grow on glycerol. Five revertants of a pb deletion mutant that grow on glycerol were analysed. They were shown to carry mutations in the glycerol kinase gene. These are missense mutations located in parts of the g/pK gene that could encode regions important for the activity of glycerol kinase. The results strongly suggest that the main effect of the PTS on glycerol utilization in B. subtilss is mediated via glycerol kinase.

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An inverted repeat preceding the Bacillus subtilis glpD gene is a conditional terminator of transcription

Cited by 26 publications

References 20 publications

The glpP and glpF genes of the glycerol regulon in Bacillus subtilis

The glpP and glpF genes of the glycerol regulon in Bacillus subtilis

The aprE leader is a determinant of extreme mRNA stability in Bacillus subtilis

Mutations in the glycerol kinase gene restore the ability of a ptsGHI mutant of Bacillus subtilis to grow on glycerol

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