The need for organizations to adapt to constant change means the challenges of implementing an agile strategy. Therefore, the purpose of the study is to analyze the role of agile women leadership and team effectiveness by looking into the mediating effect of interpersonal trust based on a cross-sectional quantitative study with a sample of 269 employees from Poland and Turkey. Questionnaires were distributed to individuals in companies having women leaders or managers. The three questionnaires required the respondents to answer questions regarding the perception of agile leadership, trust and team effectiveness. By using SPSS, demographics, descriptive statistics and tests of normality were determined. Smart PLS version 3.0 was used for confirmatory factor analysis, internal accuracy and validity estimates, hypothesis checking and mediation testing. Results of PLS-SEM indicated interpersonal trust has a full mediation role between agile women leadership in shaping team effectiveness. The population of this study are working for organizations of just two countries; hence, the generalizability of the findings to other settings is unknown. Our findings contribute to the literature on women agile leadership and team effectiveness by demonstrating how the growth in trust to managers contributes to the emergence of team effectiveness and the agile leadership trend over time. This study will therefore contribute to the understanding of organized teams’ effectiveness in the perspective of agile women leadership and trust of supervisors.