An investigation into blood microbiota and its potential association with Bacterial Chondronecrosis with Osteomyelitis (BCO) in Broilers

Mandal, Rabindra K.; Jiang, Tieshan; Alrubaye, Adnan A.K.; Rhoads, David B.; Wideman, Robert F.; Zhao, Jiangchao; Pevzner, I. Y.; Kwon, Young Min

doi:10.1038/srep25882

Cited by 59 publications

(70 citation statements)

References 44 publications

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“…Most of the blood bacterial DNA was found located in the BC (93.74%), while RBCs contain more bacterial DNA (6.23%) than the plasma (0.03%) [14]. A significant number of bacterial species were also detected in the blood of healthy chickens [16] …”

Section: Tedeschi Et Al Reported On Incorporation Of Nucleosides In mentioning

confidence: 83%

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Cultural Isolation and Characteristics of the Blood Microbiome of Healthy Individuals

Panaiotov¹,

Filevski²,

Equestre³

et al. 2018

AiM

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Background: On the analogy of the non-pathogenic microbiota found in oral cavity, skin and gastrointestinal tract, existence of blood microbiota was confirmed by DNA sequencing, but never deeply characterized. Hypothesis for the existence of dormant blood microbiota in healthy humans have been arisen and single species have been isolated. The aim of our study was to resuscitate and investigate the biodiversity of bacterial and fungal dormant blood microbiota in healthy individuals by blood culturing and NGS DNA sequencing. Results: Twenty eight blood samples of healthy individuals, seven for each blood type, were studied. Several culture media were tested. Blood microbiota resuscitation was performed in BHI broth supplemented with vitamin K 1 mg/ml, 2% sucrose, 0.25% sodium citrate and 0.2% yeastolate at 43˚C for 72 h. All tested blood samples were culture positive, as confirmed by Gram staining and TEM. TEM images demonstrated well defined cell structures. Analysis for bacterial and eukaryotic species was performed by 16S rRNA and ITS2 targeted sequencing. The obtained sequences were clustered (≥97% identity) in Operational Taxonomic Units (OTUs). Among cultured and uncultured samples we identified OTUs similarity with 47 bacterial orders belonging to 15 phyla and 39 fungi orders blonging to 2 phyla. For the first time we demonstrated isolation and sequencing identification of fungal blood microbiota in healthy individuals. Blood-group differences were identified among the bacterial microbiome compositions. Conclusion: The dormant blood microbiome is innate of the healthy individuals. Interventional strategies to bind the host blood microbiome with the states of health and disease remain an unmet research goal.

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Section: Tedeschi Et Al Reported On Incorporation Of Nucleosides In mentioning

confidence: 83%

“…We note, that some phyla are enriched by the applied resuscitation strategy according to the OTUs combined abundancy (Table 1). Other studies also identified Proteobacteria as predominant in the blood of healthy individuals [14], healthy broilers [16] and cats [17].…”

Section: Resultsmentioning

confidence: 99%

Cultural Isolation and Characteristics of the Blood Microbiome of Healthy Individuals

Panaiotov¹,

Filevski²,

Equestre³

et al. 2018

AiM

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“…The possible contamination of the DNA might be the reason for getting Rhodococcus, Pseudomonas or microbacterium, Bacillus and Sphin- gomonas in bird blood where we were expecting rickettsial reads (Weiss et al, 2014). We were not ruling out blood as sterile as there were reports of microbiota detected in bird blood (Mandal et al, 2016). Although, our results rule out possibility of migratory birds being reservoir host, birds can be rickettsiamic with Rickettsia helvetica (Hornok et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

The microbiome of neotropical ticks parasitizing on passerine migratory birds

Budachetri

Williams

Mukherjee

et al. 2017

Ticks and Tick-borne Diseases

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Seasonal migration of passerine birds between temperate North America and tropical Central and South America is an ecological phenomenon. Migration of birds has been associated with the introduction of ectoparasites like ticks or tick-borne pathogens across the avian migration routes. In this study, the microbial diversity was determined in the ticks and bird DNA samples using 454 pyrosequencing of bacterial 16S rRNA gene. Tick DNA samples showed the dominance of genera Lactococcus, Francisella, Raoultella, Wolbachia and Rickettsia across all the ticks, but birds DNA did not share common microbial diversity with ticks. Furthermore, “Candidatus Rickettsia amblyommii” infection in the 91 ticks collected off the songbirds was also quantified by qPCR assay. Interestingly, “Candidatus R. amblyommii” was tested positive in 24 ticks (26% infection), and infection varied from as low as three copies to thousands of copies, but bird blood samples showed no amplification. Our results provide evidence that songbirds serve as transport carrier for immature ticks, and less likely to be a reservoir for “Candidatus R. amblyommii”.

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“…General pipelines for data analysis was previously described in details (Mandal et al, 2016). Forward and reverse ends sequences were joined together by using join_paired_ends.py command followed by formatting barcodes using customized Perl script, before extracting barcodes using extract_barcodes.py option.…”

Section: Methodsmentioning

confidence: 99%

Characterization of the Culturable Subpopulations of Lactobacillus in the Chicken Intestinal Tract as a Resource for Probiotic Development

Adhikari

Kwon

2017

Front. Microbiol.

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To gain better understanding of the distributions of the culturable Lactobacillus species in the chicken intestinal tract, we collected ceca, and distal ileum from 10 3-weeks-old broiler chickens. Lactobacillus strains from cecal lumen contents (M-CL), and those associated with mucosa of ceca (M-CM) and ileum (M-IM) were recovered on de Man, Rogosa and Sharpe (MRS) agar plates, and used for microbiota analysis. The total cecal content (T-CL) was also used directly for microbiota analysis. We purposefully focused on MRS-recovered populations to gain understanding of the culturable subpopulations of Lactobacillus, since the culturability is an important phenotype in order to exploit the chicken gut microbiota as a resource for development of probiotics. The V1–V3 regions of 16S rRNA gene was amplified from genomic DNA samples, and the pooled amplicons were analyzed by MiSeq sequencing with paired-end read 300 cycle option. Among MRS groups, Firmicutes were significantly higher in M-IM and M-CL as compared to M-CM, whereas Proteobacteria were significantly higher in M-CM as compared to M-IM and M-CL at p < 0.05. Among Lactobacillus, L. salivarius (36%) and L. johnsonii (21%) were higher in M-IM as compared to M-CL (L. salivarius, 28%; L. johnsonii, 15%), and M-CM (L. salivarius, 20%; L. johnsonii, 11%). L. crispatus was found significantly higher in M-CL as compared to M-IM (p < 0.01) whereas L. gasseri was found significantly higher in M-IM as compared to M-CM (p < 0.05). L. aviarius, and L. fornicalis were only observed in T-CL. In summary, Lactobacillus populations recovered on MRS vary with different regions and locations in chicken GIT, which might indicate their distinct functional roles in different gastrointestinal tract (GIT) niches, and some species of Lactobacillus are not culturable on MRS agar media. This study is the first attempt to define culturable Lactobacillus subpopulations in the chicken intestinal tract comprehensively using 16S rRNA gene profiling, and the findings of this study will be used as a platform to develop a new strategy for isolation of effective Lactobacillus probiotic candidates based on comparative analyses of chicken gut microbiota.

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An investigation into blood microbiota and its potential association with Bacterial Chondronecrosis with Osteomyelitis (BCO) in Broilers

Cited by 59 publications

References 44 publications

Cultural Isolation and Characteristics of the Blood Microbiome of Healthy Individuals

Cultural Isolation and Characteristics of the Blood Microbiome of Healthy Individuals

The microbiome of neotropical ticks parasitizing on passerine migratory birds

Characterization of the Culturable Subpopulations of Lactobacillus in the Chicken Intestinal Tract as a Resource for Probiotic Development

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