An investigation into producing Botryococcus braunii in a tubular photobioreactor

Abstract

BACKGROUND Botryococcus braunii is a eukaryotic photosynthetic microorganism, requiring light, water, and inorganic nutrients for growth. It can convert CO2 into biomass and bioactive compounds with applications in food, pharmaceutical and cosmetic industries. Parameters such as inorganic carbon and nitrogen sources, vitamins, and salts are important factors regulating algal growth and biomass composition. RESULTS It was possible to observe the influence of salinity on growth and biomass composition of B. braunii. Chu medium was found to be the best medium for growth. In a tubular photobioreactor, biomass concentration of 4963 mg L−1 and productivity of 173.3 mg L−1 d−1 was achieved at 25 °C and 71 µmol of photons m−2 s−1, with the use of a nylon sphere to improve agitation, and with adjustment of nitrogen and phosphorus concentrations by daily addition of K2HPO4 and NaNO3 according to the cell growth. Concentration of lipids was found in a range from 32.6 to 36.9%, and oleic acid (29.4–34.7%) and linoleic acid (19.8–23.7%) were the dominant fatty acids. CONCLUSION This study showed the feasibility of using Chu medium for the growth of B. braunii, and the importance of employing a nylon sphere and adjusting nitrogen and phosphorus concentrations in the culture medium of a tubular photobioreactor. © 2016 Society of Chemical Industry

Tertiary treatment of dairy industry wastewater with production of Chlorella vulgaris biomass: evaluation of effluent dilution

“…Finally, the lipid fraction was recovered in petroleum ether. After the conversion of fatty acids into their corresponding methyl esters (Hartman and Lago, 1973), the analysis of fatty acid methyl esters was carried out in a gas chromatograph, model 7890 (Agillent Technologies, USA), equipped with a split/splitless injector and FID detector (flame ionization detector) in accordance with Pérez-Mora et al (2016). The identification of fatty acids in the samples was carried out by comparing the retention times with those obtained in standards present in "37 Component FAME Mix" (Supelco).…”

Effects of incident light intensity and light path length on cell growth and oil accumulation in Botryococcus braunii (Chlorophyta)

“…Numerous studies on the growth and hydrocarbon production of B. braunii have been conducted. The effects of various nutritional and environmental factors such as nitrate, phosphate, micronutrients, carbon source, temperature, salinity, and pH on the growth and metabolism of B. braunii have been investigated, as well as the optimization of photobioreactor types . When cultivated in photoautotrophic conditions, light is one of the most important factors as it is the essential energy source for the synthesis of algal biomass and metabolites.…”

Aplicação do extrato da microalga <i>Botryococcus braunii</i> em formulações cosméticas fotoprotetoras

“…A ação antioxidante das microalgas tem sido relacionada à presença de compostos fenólicos e carotenoides. No entanto, atribui-se a referida ação mais aos carotenoides, pois, apesar de alguns estudos já terem relatado classes de flavonoides, ainda há pouca informação sobre a presença de substâncias fenólicas em microalgas (CHOOCHOTE;SUKLAMPOO, OCHAIKUL, 2014;GOIRIS et al, 2012;MAADANE et al, 2015;MARTINS et al, 2014;SAFAFAR et al, 2015;SARANYA et al, 2014;WANG et al, 2015;WARD, 2016 (BANERJEE et al, 2002;DAYANANDA et al, 2007a;DEMURA et al, 2014;FANG et al, 2004;FURUHASHI et al, 2016a;HIROSE et al, 2013;METZGER, LARGEAU, 2005;PÉREZ-MORA et al, 2016). Esta microalga cresce formando colônias irregulares de 30 µm a 2 mm de tamanho, sendo constituídas por células elípticas de 6 a 20 µm de diâmetro e comprimento de 2,5 a 8 µm.…”

“…Esta microalga cresce formando colônias irregulares de 30 µm a 2 mm de tamanho, sendo constituídas por células elípticas de 6 a 20 µm de diâmetro e comprimento de 2,5 a 8 µm. Suas células são unidas por uma matriz mucilaginosa transparente, sendo possível existirem várias colônias ligadas umas às outras por meio de finos fios hialinos (ACHITOUV et al, 2004;LI, QIN, 2005;PÉREZ-MORA et al, 2016). -HOTHALY et al, 2016;BANERJEE et al, 2002;DAYANANDA et al, 2007aDAYANANDA et al, , 2007bFURUHASHI et al, 2016b;KOLLER et al, 2012;METZGER, LARGEAU, 2005;XIN et al, 2010).…”

“…Os dados foram avaliados pelo programa Minitab ® , versão 17, em triplicata, empregando ANOVA (modelo linear generalizado) ou delineamento experimental do tipo fatorial, compreendendo o nível de significância de 5% (p≤0,05) para a determinação de resultados estatisticamente significativos. tempo, a correção dos elementos fósforo e nitrogênio no meio, uma vez que fazem parte da composição elementar da biomassa de B. braunii (PÉREZ-MORA et al, 2016…”