An investigation into the possible changes in the microbial population of soils stored at ?15�C

Abstract

the effect of storage of solls at sub-freezing t e m p e r a t u r e s in connection w i t h their work on the s t a t u s of mineral nitrogen and mineralizability of nitrogen

A research note on the assessment of optimum conditions for preparing soils for bioremediation feasibility testing

“…Therefore, the composition of heterotrophic bacterial species growing on TSA at each time period may change, with specific groups increasing while others decrease. For example, in soils stored at −15°C, cold sensitive‐microorganisms may be killed, but the rate of development of cold resistant bacteria may increase leading to an increased total count (Grossbard & Hall, ).…”

“…Two naphthalene contaminated soils from two additional contaminated sites were also used in the study: One from South Extraction techniques References 5-10 g soil + 100 ml sterile water; shake uniformly for 5 min; serial dilutions (sterile water) Waksman (1922) 5 g soil + 50 ml sterile water; sonic vibration for 5 min Stevenson (1958) 50 g soil + 450 ml saline (0.75%); shake-mechanical end-over-end shaker (ca. 17 rpm) 30 min; serial dilutions (sterile water) Grossbard and Hall (1964) 50 g soil + 450 ml 0.001 M Tris buffer; waring blender (16-18,000 rpm) for 1-5 min. Kanazawa, Takeshima, and Ohta (1986) 1 g soil + 24 ml filtered, reverse osmosis water; shake by hand for 1 min, stand 2 min; upper 10 ml removed and serial dilutions were prepared Bogardt and Hemmingsen (1992) 10 g soil + 100 ml sterile 0.2% tetrasodium pyrophosphate; shake 150 rpm for 30 min, stand 2-5 min.…”