An investigation into the relationship between the metabolic profile of follicular fluid, oocyte developmental potential, and implantation outcome

Wallace, Martina; Cottell, Evelyn; Gibney, Michael J.; McAuliffe, Fionnuala M.; Wingfield, Mary; Brennan, Lorraine

doi:10.1016/j.fertnstert.2012.01.122

Cited by 127 publications

(109 citation statements)

References 51 publications

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“…Similarly, high ApoB in human follicular fluid is associated with better quality embryos and higher pregnancy rates (Gautier et al 2010). However, two recent studies have shown that increased HDL and ApoAI levels were associated with failure of oocytes to cleave and decreased the numbers of good quality embryos (Valckx et al 2012, Wallace et al 2012. Furthermore, it is unclear whether lipoproteins found in follicular fluid influence oocyte quality via their ability to deliver lipid substrates such as triacylglycerol or whether other components of these particles, namely the surrounding apolipoproteins, which are known to have scavenging properties, protect cells from oxidative stress.…”

Section: Fatty Acid Supply To the Cocmentioning

confidence: 99%

Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation

2014

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Metabolism and ATP levels within the oocyte and adjacent cumulus cells are associated with quality of oocyte and optimal development of a healthy embryo. Lipid metabolism provides a potent source of energy and its importance during oocyte maturation is being increasingly recognised. The triglyceride and fatty acid composition of ovarian follicular fluid has been characterised for many species and is influenced by nutritional status (i.e. dietary fat, fasting, obesity and season) as well as lactation in cows. Lipid in oocytes is a primarily triglyceride of specific fatty acids which differ by species, stored in distinct droplet organelles that re-localise during oocyte maturation. The presence of lipids, particularly saturated vs unsaturated fatty acids, in in vitro maturation systems affects oocyte lipid content as well as developmental competence. Triglycerides are metabolised by lipases that have been localised to cumulus cells as well as oocytes. Fatty acids generated by lipolysis are further metabolised by b-oxidation in mitochondria for the production of ATP. b-oxidation is induced in cumulus-oocyte complexes (COCs) by the LH surge, and pharmacological inhibition of b-oxidation impairs oocyte maturation and embryo development. Promoting b-oxidation with L-carnitine improves embryo development in many species. Thus, fatty acid metabolism in the mammalian COC is regulated by maternal physiological and in vitro environmental conditions; and is important for oocyte developmental competence.

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Section: Fatty Acid Supply To the Cocmentioning

confidence: 99%

Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation

2014

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“…Several other studies have examined FF composition using NMR technology and found associations between lipoproteins and oocyte/embryo outcomes [61][62][63][64]. However, these studies were limited by the absence of a Bone-follicle, oneembryo^design and most were not conducted in humans.…”

Section: Geometric Mean Concentration (µMol/l) Hdl-c Subfractions (Nm)mentioning

confidence: 99%

Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients

Kim

Bloom

Browne

et al. 2016

J Assist Reprod Genet

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Purpose Follicular redox balance is likely to be important for embryo quality during in vitro fertilization (IVF), and the anti-oxidative high desity lipoprotein (HDL) particle is the sole lipoprotein measured in follicular fluid (FF). Therefore, we investigated FF HDL particle components as predictors of embryo quality during IVF. Methods Two research follicles collected from each participant were individually tracked, and 103 women having at least one developed embryo were included in the analysis. Concentrations of 15 non-cholesterol HDL particle components and 26 HDL-cholesterol (HDL-C) particle size subfractions were determined. Embryo quality was assessed for embryo cell number, embryo fragmentation, and embryo symmetry. Multivariable Poisson regression with a sandwich variance estimator was used to evaluate associations between HDL particle components and embryo quality, adjusted for covariates. Results Higher γ-tocopherol concentration was associated with less embryo fragmentation (relative risk [RR] = 4.43; 95 % confidence interval [CI] 1.78, 11.06), and higher apolipoprotein A-1 concentration was associated with full embryo symmetry (RR = 3.92; 95 % CI 1.56, 9.90). Higher concentrations of HDL-C subfractions in the large and medium particle size ranges were associated with poorer embryo quality. Conclusions FF HDL lipophilic micronutrients and protein components, as well as HDL-C particle size, may be important predictors of embryo quality during IVF.

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“…Investigation of follicular fluid composition as a possible predictor of both oocyte developmental competence and embryo viability has increased in recent years (Wu et al 2007, Berker et al 2009, Revelli et al 2009, Piñ ero-Sagredo et al 2010, Wallace et al 2012. Follicular fluid contains important metabolites that can be critical for oocyte growth and development and provides important information about the growth and differentiation of the follicle (Edwards 1974).…”

Section: Introductionmentioning

confidence: 99%

Metabolic profiling of human follicular fluid identifies potential biomarkers of oocyte developmental competence

O’Gorman¹,

Wallace²,

Cottell³

et al. 2013

Reproduction

109

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The use of metabolomic based techniques to aid oocyte and embryo selection has gained attention in recent years. Previous work from our laboratory has demonstrated that the 1 H NMR-based metabolic profile of follicular fluid correlates with oocyte developmental potential. Patients undergoing IVF at the Merrion Fertility Clinic had follicular fluid collected at the time of oocyte retrieval. The fatty acid composition of follicular fluid from follicles where oocytes fertilised and developed into multi-cell embryos (nZ15) and from oocytes that fertilised normally but failed to cleave (nZ9) (cleaved vs non-cleaved) was compared. Statistical analysis was performed on the data using univariate and multivariate techniques. Analysis of the fatty acid composition revealed that there were nine fatty acids significantly different between follicular fluid from the cleaved and the non-cleaved sample groups.Of particular interest were the higher concentration of total saturated (PZ0.03) and the lower concentration of total polyunsaturated fatty acids in the non-cleaved sample group (PZ0.001). Random forest classification models were used to predict successful cleavage in follicular fluid samples producing models with errors rates of !10%. Receiver operating characteristic analysis demonstrated that the model had good predictability with an area under the curve of 0.96. The panel of fatty acid biomarkers identified in this study indicates that the fatty acid composition of follicular fluid may be more predictive in comparison to other previously identified biomarkers. Following validation in a larger cohort, these biomarkers may have the potential to be used in fertility clinics to aid the selection of oocytes in the future.

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An investigation into the relationship between the metabolic profile of follicular fluid, oocyte developmental potential, and implantation outcome

Cited by 127 publications

References 51 publications

Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation

Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation

Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients

Metabolic profiling of human follicular fluid identifies potential biomarkers of oocyte developmental competence

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